(Circular) (Six-base) MAPSORT of: pFN21AE6692.seq Check: 1770 from: 1 to: 5692 pFN21AE6692 5692 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2766 3502 278 Size: 53 83 186 936 1230 736 2468 Fragments arranged by size: 2468 1230 936 736 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 1937 Size: 1098 4594 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3755 5139 5407 1632 Size: 2123 1384 268 1917 Fragments arranged by size: 2123 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 2271 2730 2934 4591 5568 1203 Size: 1068 459 204 1657 977 1327 Fragments arranged by size: 1657 1327 1068 977 459 204 AgeI A'CCGG_T Cuts at: 2802 4523 2802 Size: 1721 3971 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 2830 2851 3435 5007 2830 Size: 21 584 1572 3515 Fragments arranged by size: 3515 1572 584 21 ApaI G_GGCC'C Cuts at: 2263 2263 Size: 5692 ApaLI G'TGCA_C Cuts at: 2678 3749 4905 2678 Size: 1071 1156 3465 Fragments arranged by size: 3465 1156 1071 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AscI GG'CGCG_CC Cuts at: 2323 2323 Size: 5692 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2263 2563 3017 1870 Size: 73 168 152 300 454 4545 Fragments arranged by size: 4545 454 300 168 152 73 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2309 2741 2752 3013 4468 Size: 325 217 627 522 432 11 261 1455 Cuts at: 4468 618 Size: 1842 Fragments arranged by size: 1842 1455 627 522 432 325 261 217 11 BanII G_rGCy'C Cuts at: 1391 2112 2263 2529 3011 1391 Size: 721 151 266 482 4072 Fragments arranged by size: 4072 721 482 266 151 BbeI G_GCGC'C Cuts at: 2313 2745 2756 2313 Size: 432 11 5249 Fragments arranged by size: 5249 432 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4721 BbvCI CC'TCA_GC Cuts at: 2693 2693 Size: 5692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2180 2423 2590 Size: 107 122 71 631 605 508 243 167 Cuts at: 2590 2750 4303 136 Size: 160 1553 1525 Fragments arranged by size: 1553 1525 631 605 508 243 167 160 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 2057 2263 2297 2682 3753 4909 1790 Size: 267 206 34 385 1071 1156 2573 Fragments arranged by size: 2573 1156 1071 385 267 206 34 BmgBI CAC'GTC Cuts at: 1318 2272 2731 1318 Size: 954 459 4279 Fragments arranged by size: 4279 954 459 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2560 3423 4381 Size: 416 53 83 399 24 1143 863 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1143 958 863 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2873 2885 4352 1239 Size: 1112 522 12 1467 2579 Fragments arranged by size: 2579 1467 1112 522 12 Bpu10I CC'TnA_GC Cuts at: 2693 2693 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2447 3817 4682 4980 2447 Size: 1370 865 298 3159 Fragments arranged by size: 3159 1370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2241 2443 2659 2935 3483 493 Size: 711 1037 202 216 276 548 2702 Fragments arranged by size: 2702 1037 711 548 276 216 202 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2310 2385 2742 2753 Size: 53 83 186 936 777 75 357 11 Cuts at: 2753 2763 3499 3881 4516 275 Size: 10 736 382 635 1451 Fragments arranged by size: 1451 936 777 736 635 382 357 186 83 75 53 11 10 BsaWI w'CCGG_w Cuts at: 2802 4125 4523 4797 4944 5355 2802 Size: 1323 398 274 147 411 3139 Fragments arranged by size: 3139 1323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 1178 Size: 30 165 30 1059 30 4378 Fragments arranged by size: 4378 1059 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 1186 Size: 1144 4548 BseYI C'CCAG_C Cuts at: 2644 4895 5391 2644 Size: 2251 496 2945 Fragments arranged by size: 2945 2251 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2936 1163 Size: 573 1200 3919 Fragments arranged by size: 3919 1200 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 2301 2575 2670 3142 3903 Size: 275 186 105 195 274 95 472 761 Cuts at: 3903 4931 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 472 275 274 195 186 105 95 BsiHKAI G_wGCw'C Cuts at: 2442 2463 2664 2682 2778 3753 3838 4909 2442 Size: 21 201 18 96 975 85 1071 3225 Fragments arranged by size: 3225 1071 975 201 96 85 21 18 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2112 2263 2297 2442 2463 2529 Size: 399 267 55 151 34 145 21 66 Cuts at: 2529 2664 2682 2778 3011 3753 3838 4909 1391 Size: 135 18 96 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 267 233 151 145 135 96 85 66 55 34 21 18 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2367 2640 2694 2715 3047 844 Size: 1523 273 54 21 332 3489 Fragments arranged by size: 3489 1523 332 273 54 21 BsrBI CCG'CTC Cuts at: 1394 1823 2320 2335 3579 1394 Size: 429 497 15 1244 3507 Fragments arranged by size: 3507 1244 497 429 15 BsrDI GCAATG_nn' Cuts at: 65 1248 4187 4361 65 Size: 1183 2939 174 1396 Fragments arranged by size: 2939 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2300 2755 2802 4336 4523 1700 Size: 110 490 455 47 1534 187 2869 Fragments arranged by size: 2869 1534 490 455 187 110 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 2093 2162 2164 2199 2279 2323 2515 2531 Size: 168 69 2 35 80 44 192 16 Cuts at: 2531 2710 2885 1925 Size: 179 175 4732 Fragments arranged by size: 4732 192 179 175 168 80 69 44 35 16 2 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 2686 794 Size: 1892 3800 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstXI CCAn_nnnn'nTGG Cuts at: 2413 2413 Size: 5692 BstYI r'GATC_y Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687 Size: 1050 367 385 17 1452 86 12 346 Cuts at: 5687 1972 Size: 1977 Fragments arranged by size: 1977 1452 1050 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2289 2569 2809 513 Size: 552 1224 280 240 3396 Fragments arranged by size: 3396 1224 552 280 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2141 487 Size: 683 56 915 4038 Fragments arranged by size: 4038 915 683 56 BtsI GCAGTG_nn' Cuts at: 2190 2969 3208 4002 4022 2190 Size: 779 239 794 20 3860 Fragments arranged by size: 3860 794 779 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2193 2298 Size: 54 1425 50 275 18 273 90 105 Cuts at: 2298 2403 2415 2505 2572 2667 3139 4028 5657 Size: 105 12 90 67 95 472 889 1629 Cuts at: 5657 8 Size: 43 Fragments arranged by size: 1629 1425 889 472 275 273 105 105 95 90 90 67 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2103 2298 2572 2667 3139 1537 Size: 275 291 195 274 95 472 4090 Fragments arranged by size: 4090 472 291 275 274 195 95 EarI CTCTTCn'nnn_ Cuts at: 1353 3622 1353 Size: 2269 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2483 4279 4653 4799 387 Size: 1064 138 894 1796 374 146 1280 Fragments arranged by size: 1796 1280 1064 894 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2873 2885 3755 4352 5139 5407 Size: 393 719 522 12 870 597 787 268 Cuts at: 5407 1239 Size: 1524 Fragments arranged by size: 1524 870 787 719 597 522 393 268 12 EcoO109I rG'GnC_Cy Cuts at: 2116 2259 2260 2356 2116 Size: 143 1 96 5452 Fragments arranged by size: 5452 143 96 1 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FseI GG_CCGG'CC Cuts at: 2304 2304 Size: 5692 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 2045 2066 2081 2221 2313 2596 2745 2756 Size: 367 21 15 140 92 283 149 11 Cuts at: 2756 4839 1678 Size: 2083 2531 Fragments arranged by size: 2531 2083 367 283 149 140 92 21 15 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 2717 2749 4333 Size: 32 135 32 890 32 223 32 1584 Cuts at: 4333 4365 4407 4439 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1584 890 223 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KasI G'GCGC_C Cuts at: 2309 2741 2752 2309 Size: 432 11 5249 Fragments arranged by size: 5249 432 11 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2417 10 Size: 54 1425 343 363 222 3285 Fragments arranged by size: 3285 1425 363 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 3650 4009 4168 518 Size: 648 2484 359 159 2042 Fragments arranged by size: 2484 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2571 2770 2811 2920 3108 3785 4933 5178 Size: 965 199 41 109 188 677 1148 245 Cuts at: 5178 1606 Size: 2120 Fragments arranged by size: 2120 1148 965 677 245 199 188 109 41 NaeI GCC'GGC Cuts at: 2302 2302 Size: 5692 NarI GG'CG_CC Cuts at: 2310 2742 2753 2310 Size: 432 11 5249 Fragments arranged by size: 5249 432 11 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5140 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NgoMIV G'CCGG_C Cuts at: 2300 2300 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 2572 3139 2572 Size: 567 5125 NspI r_CATG'y Cuts at: 1132 1229 2863 3050 4595 1132 Size: 97 1634 187 1545 2229 Fragments arranged by size: 2229 1634 1545 187 97 PasI CC'CwG_GG Cuts at: 1348 2121 1348 Size: 773 4919 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 2830 1108 Size: 1722 3970 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PmlI CAC'GTG Cuts at: 2241 2443 2241 Size: 202 5490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2116 2356 2116 Size: 240 5452 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PspOMI G'GGCC_C Cuts at: 2259 2259 Size: 5692 PstI C_TGCA'G Cuts at: 830 2819 3044 830 Size: 1989 225 3478 Fragments arranged by size: 3478 1989 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2920 2920 Size: 5692 SacII CC_GC'GG Cuts at: 2572 2812 2572 Size: 240 5452 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SanDI GG'GwC_CC Cuts at: 2116 2116 Size: 5692 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SexAI A'CCwGG_T Cuts at: 2673 2829 2673 Size: 156 5536 SfcI C'TryA_G Cuts at: 826 1045 2815 3040 4175 4856 5047 826 Size: 219 1770 225 1135 681 191 1471 Fragments arranged by size: 1770 1471 1135 681 225 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2423 2423 Size: 5692 SfoI GGC'GCC Cuts at: 2311 2743 2754 2311 Size: 432 11 5249 Fragments arranged by size: 5249 432 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 2755 1700 Size: 1055 4637 SmaI CCC'GGG Cuts at: 1872 2113 2265 3019 1872 Size: 241 152 754 4545 Fragments arranged by size: 4545 754 241 152 SmlI C'TyrA_G Cuts at: 820 1017 1943 2462 3796 4697 4959 820 Size: 197 926 519 1334 901 262 1553 Fragments arranged by size: 1553 1334 926 901 519 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 2049 1517 Size: 532 5160 StyI C'CwwG_G Cuts at: 513 1065 2406 3131 513 Size: 552 1341 725 3074 Fragments arranged by size: 3074 1341 725 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3719 3736 3889 4074 4519 325 Size: 1255 2139 17 153 185 445 1498 Fragments arranged by size: 2139 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5652 5684 5652 Size: 32 5660 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 2111 2263 3017 1870 Size: 241 152 754 4545 Fragments arranged by size: 4545 754 241 152 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2764 3500 276 Size: 53 83 186 936 1230 736 2468 Fragments arranged by size: 2468 1230 936 736 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SrfI SwaI XcmI