Restriction map for
pFN21AE6692
(Linear) (Six-base) MAPSORT of: KIEE6692.seq Check: 3142 from: 1 to: 987
KIEE6692.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 761 987
Size: 761 226
AflIII A'CryG_T
Cuts at: 0 266 725 929 987
Size: 266 459 204 58
Fragments arranged by size:
459 266 204 58
AgeI A'CCGG_T
Cuts at: 0 797 987
Size: 797 190
AlwNI CAG_nnn'CTG
Cuts at: 0 825 846 987
Size: 825 21 141
Fragments arranged by size:
825 141 21
ApaI G_GGCC'C
Cuts at: 0 258 987
Size: 258 729
ApaLI G'TGCA_C
Cuts at: 0 673 987
Size: 673 314
AscI GG'CGCG_CC
Cuts at: 0 318 987
Size: 318 669
AvaI C'yCGr_G
Cuts at: 0 106 258 558 987
Size: 106 152 300 429
Fragments arranged by size:
429 300 152 106
BanI G'GyrC_C
Cuts at: 0 304 736 747 987
Size: 304 432 11 240
Fragments arranged by size:
432 304 240 11
BanII G_rGCy'C
Cuts at: 0 107 258 524 987
Size: 107 151 266 463
Fragments arranged by size:
463 266 151 107
BbeI G_GCGC'C
Cuts at: 0 308 740 751 987
Size: 308 432 11 236
Fragments arranged by size:
432 308 236 11
BbvCI CC'TCA_GC
Cuts at: 0 688 987
Size: 688 299
BglI GCCn_nnn'nGGC
Cuts at: 0 175 418 585 745 987
Size: 175 243 167 160 242
Fragments arranged by size:
243 242 175 167 160
Bme1580I G_kGCm'C
Cuts at: 0 52 258 292 677 987
Size: 52 206 34 385 310
Fragments arranged by size:
385 310 206 52 34
BmgBI CAC'GTC
Cuts at: 0 267 726 987
Size: 267 459 261
Fragments arranged by size:
459 267 261
BmrI ACTGGGnnnn_n'
Cuts at: 0 555 987
Size: 555 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 868 880 987
Size: 346 522 12 107
Fragments arranged by size:
522 346 107 12
Bpu10I CC'TnA_GC
Cuts at: 0 688 987
Size: 688 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 987
Size: 442 545
BsaAI yAC'GTr
Cuts at: 0 236 438 654 930 987
Size: 236 202 216 276 57
Fragments arranged by size:
276 236 216 202 57
BsaHI Gr'CG_yC
Cuts at: 0 305 380 737 748 758 987
Size: 305 75 357 11 10 229
Fragments arranged by size:
357 305 229 75 11 10
BsaWI w'CCGG_w
Cuts at: 0 797 987
Size: 797 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 987
Size: 457 30 500
Fragments arranged by size:
500 457 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 987
Size: 325 662
BseYI C'CCAG_C
Cuts at: 0 639 987
Size: 639 348
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 987
Size: 931 56
BsiEI CG_ry'CG
Cuts at: 0 101 296 570 665 987
Size: 101 195 274 95 322
Fragments arranged by size:
322 274 195 101 95
BsiHKAI G_wGCw'C
Cuts at: 0 437 458 659 677 773 987
Size: 437 21 201 18 96 214
Fragments arranged by size:
437 214 201 96 21 18
Bsp1286I G_dGCh'C
Cuts at: 0 52 107 258 292 437 458 524 659
Size: 52 55 151 34 145 21 66 135
Cuts at: 659 677 773 987
Size: 18 96 214
Fragments arranged by size:
214 151 145 135 96 66 55 52
34 21 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 635 689 710 987
Size: 362 273 54 21 277
Fragments arranged by size:
362 277 273 54 21
BsrBI CCG'CTC
Cuts at: 0 315 330 987
Size: 315 15 657
Fragments arranged by size:
657 315 15
BsrFI r'CCGG_y
Cuts at: 0 295 750 797 987
Size: 295 455 47 190
Fragments arranged by size:
455 295 190 47
BssHII G'CGCG_C
Cuts at: 0 88 157 159 194 274 318 510 526
Size: 88 69 2 35 80 44 192 16
Cuts at: 526 705 880 987
Size: 179 175 107
Fragments arranged by size:
192 179 175 107 88 80 69 44
35 16 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 681 987
Size: 681 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 408 987
Size: 408 579
BtgI C'CryG_G
Cuts at: 0 284 564 804 987
Size: 284 280 240 183
Fragments arranged by size:
284 280 240 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 136 987
Size: 136 851
BtsI GCAGTG_nn'
Cuts at: 0 185 964 987
Size: 185 779 23
Fragments arranged by size:
779 185 23
EaeI y'GGCC_r
Cuts at: 0 98 188 293 398 410 500 567 662
Size: 98 90 105 105 12 90 67 95
Cuts at: 662 987
Size: 325
Fragments arranged by size:
325 105 105 98 95 90 90 67
12
EagI C'GGCC_G
Cuts at: 0 98 293 567 662 987
Size: 98 195 274 95 325
Fragments arranged by size:
325 274 195 98 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 478 987
Size: 478 509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 868 880 987
Size: 346 522 12 107
Fragments arranged by size:
522 346 107 12
EcoO109I rG'GnC_Cy
Cuts at: 0 111 254 255 351 987
Size: 111 143 1 96 636
Fragments arranged by size:
636 143 111 96 1
FseI GG_CCGG'CC
Cuts at: 0 299 987
Size: 299 688
HaeII r_GCGC'y
Cuts at: 0 40 61 76 216 308 591 740 751
Size: 40 21 15 140 92 283 149 11
Cuts at: 751 987
Size: 236
Fragments arranged by size:
283 236 149 140 92 40 21 15
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 712 744 987
Size: 457 32 223 32 243
Fragments arranged by size:
457 243 223 32 32
KasI G'GCGC_C
Cuts at: 0 304 736 747 987
Size: 304 432 11 240
Fragments arranged by size:
432 304 240 11
MscI TGG'CCA
Cuts at: 0 190 412 987
Size: 190 222 575
Fragments arranged by size:
575 222 190
MspA1I CmG'CkG
Cuts at: 0 566 765 806 915 987
Size: 566 199 41 109 72
Fragments arranged by size:
566 199 109 72 41
NaeI GCC'GGC
Cuts at: 0 297 987
Size: 297 690
NarI GG'CG_CC
Cuts at: 0 305 737 748 987
Size: 305 432 11 239
Fragments arranged by size:
432 305 239 11
NgoMIV G'CCGG_C
Cuts at: 0 295 987
Size: 295 692
NotI GC'GGCC_GC
Cuts at: 0 567 987
Size: 567 420
NspI r_CATG'y
Cuts at: 0 858 987
Size: 858 129
PasI CC'CwG_GG
Cuts at: 0 116 987
Size: 116 871
PflMI CCAn_nnn'nTGG
Cuts at: 0 825 987
Size: 825 162
PmlI CAC'GTG
Cuts at: 0 236 438 987
Size: 236 202 549
Fragments arranged by size:
549 236 202
PpuMI rG'GwC_Cy
Cuts at: 0 111 351 987
Size: 111 240 636
Fragments arranged by size:
636 240 111
PspOMI G'GGCC_C
Cuts at: 0 254 987
Size: 254 733
PstI C_TGCA'G
Cuts at: 0 814 987
Size: 814 173
PvuII CAG'CTG
Cuts at: 0 915 987
Size: 915 72
SacII CC_GC'GG
Cuts at: 0 567 807 987
Size: 567 240 180
Fragments arranged by size:
567 240 180
SanDI GG'GwC_CC
Cuts at: 0 111 987
Size: 111 876
SexAI A'CCwGG_T
Cuts at: 0 668 824 987
Size: 668 156 163
Fragments arranged by size:
668 163 156
SfcI C'TryA_G
Cuts at: 0 810 987
Size: 810 177
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 418 987
Size: 418 569
SfoI GGC'GCC
Cuts at: 0 306 738 749 987
Size: 306 432 11 238
Fragments arranged by size:
432 306 238 11
SgrAI Cr'CCGG_yG
Cuts at: 0 750 987
Size: 750 237
SmaI CCC'GGG
Cuts at: 0 108 260 987
Size: 108 152 727
Fragments arranged by size:
727 152 108
SmlI C'TyrA_G
Cuts at: 0 457 987
Size: 457 530
StuI AGG'CCT
Cuts at: 0 44 987
Size: 44 943
StyI C'CwwG_G
Cuts at: 0 401 987
Size: 401 586
XmaI C'CCGG_G
Cuts at: 0 106 258 987
Size: 106 152 729
Fragments arranged by size:
729 152 106
ZraI GAC'GTC
Cuts at: 0 759 987
Size: 759 228
Enzymes that do cut:
AatII AflIII AgeI AlwNI ApaI ApaLI AscI AvaI
BanI BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstXI BtgI BtgZI BtsI EaeI EagI
EciI Eco57MI EcoO109I FseI HaeII Hin4I KasI MscI
MspA1I NaeI NarI NgoMIV NotI NspI PasI PflMI
PmlI PpuMI PspOMI PstI PvuII SacII SanDI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AlfI AloI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI BsaI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI
NheI NruI NsiI PacI PciI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI