Restriction map for
pFN21AE1768
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BsrI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII HinfI
HpaI KasI KpnI Ksp632I MaeI
MamI MboII MfeI MflI MluI
MseI MslI MunI NarI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp509I
TthHB8I XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 28 CGCG CG^CG
AcyI 805 GACGCC G[GA]^CG[CT]C
AfaI 711 GTAC GT^AC
AgeI 119 ACCGGT A^CCGGT
Alw44I 294 GTGCAC G^TGCAC
ApaLI 294 GTGCAC G^TGCAC
AvaI 628 CCCGGG C^[CT]CG[GA]G
BanI 334 GGCACC G^G[CT][GA]CC
BbrPI 252 CACGTG CAC^GTG
BglI 326 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 66 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 66 GCTGAGC GC^T[ACGT]AGC
BsaAI 252 CACGTG [CT]AC^GT[GA]
BsaHI 805 GACGCC G[GA]^CG[CT]C
BsaI 532 GAGACC GGTCTC(1/5)
BsiEI 117 CGACCG CG[GA][CT]^CG
BsmAI 532 GAGAC GTCTC(1/5)
BsoBI 628 CCCGGG C^[CT]CG[GA]G
BspMI 713 ACCTGC ACCTGC(4/8)
BssSI 130 CACGAG CACGAG(-5/-1)
BstUI 28 CGCG CG^CG
BstXI 695 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 66 GCTGAGC GC^T[ACGT]AGC
DpnII 828 GATC ^GATC
EcoNI 760 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 443 CCTTGG C^C[AT][AT]GG
Hin1I 805 GACGCC G[GA]^CG[CT]C
HincII 367 GTTGAC GT[CT]^[GA]AC
HindII 367 GTTGAC GT[CT]^[GA]AC
HphI 156 GGTGA GGTGA(8/7)
KspI 27 CCGCGG CCGC^GG
MboI 828 GATC ^GATC
MvnI 28 CGCG CG^CG
NaeI 468 GCCGGC GCC^GGC
NdeII 828 GATC ^GATC
NgoMIV 468 GCCGGC G^CCGGC
PflMI 339 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 119 ACCGGT A^CCGGT
PmaCI 252 CACGTG CAC^GTG
PmlI 252 CACGTG CAC^GTG
PvuII 289 CAGCTG CAG^CTG
RsaI 711 GTAC GT^AC
SacII 27 CCGCGG CCGC^GG
Sau3AI 828 GATC ^GATC
SfaNI 745 GATGC GCATC(5/9)
SmaI 628 CCCGGG CCC^GGG
StuI 416 AGGCCT AGG^CCT
StyI 443 CCTTGG C^C[AT][AT]GG
Tsp45I 76 GTGAC ^GT[GC]AC
Van91I 339 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 628 CCCGGG C^CCGGG
XmaI 628 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 719 TCCGGA T^CCGGA
776 TCCGGA
AlwNI 340 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
730 CAGAGGCTG
ApaI 540 GGGCCC GGGCC^C
684 GGGCCC
AspHI 69 GAGCAC G[AT]GC[AT]^C
294 GTGCAC
AspI 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
310 GACACAGTC
BalI 692 TGGCCA TGG^CCA
821 TGGCCA
BpmI 386 CTCCAG CTGGAG(16/14)
362 CTGGAG
BseAI 719 TCCGGA T^CCGGA
776 TCCGGA
BseRI 49 CTCCTC GAGGAG(10/8)
793 GAGGAG
BsiHKAI 69 GAGCAC G[AT]GC[AT]^C
294 GTGCAC
BspEI 719 TCCGGA T^CCGGA
776 TCCGGA
BsrBI 171 CCGCTC CCGCTC(-3/-3)
280 GAGCGG
BsrFI 119 ACCGGT [GA]^CCGG[CT]
468 GCCGGC
BstAPI 242 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
456 GCACACCCTGC
Cfr10I 119 ACCGGT [GA]^CCGG[CT]
468 GCCGGC
DdeI 67 CTGAG C^T[ACGT]AG
265 CTCAG
DraIII 183 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
252 CACGTGGTG
DsaI 27 CCGCGG C^C[GA][CT]GG
617 CCGTGG
HgaI 487 GACGC GACGC(5/10)
805 GACGC
MaeII 221 ACGT A^CGT
253 ACGT
MaeIII 76 GTGAC ^GT[ACGT]AC
260 GTAAC
MluNI 692 TGGCCA TGG^CCA
821 TGGCCA
MroI 719 TCCGGA T^CCGGA
776 TCCGGA
MscI 692 TGGCCA TGG^CCA
821 TGGCCA
PflFI 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
310 GACACAGTC
PstI 165 CTGCAG CTGCA^G
463 CTGCAG
SfcI 165 CTGCAG C^T[GA][CT]AG
463 CTGCAG
Tth111I 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
310 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsaWI BsgI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV PpuMI Sau96I ScrFI
TseI TspRI XcmI