Restriction map for
pFN21AE1768
(Circular) (Six-base) MAPSORT of: pFN21AE1768.seq Check: 8826 from: 1 to: 5536
pFN21AE1768 5536 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 1937
Size: 942 4594
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2601 3599 4983 5251 1632
Size: 969 998 1384 268 1917
Fragments arranged by size:
1917 1384 998 969 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 2124 4367 2124
Size: 2243 3293
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 2350 2740 3279 4851 2350
Size: 390 539 1572 3035
Fragments arranged by size:
3035 1572 539 390
ApaI G_GGCC'C
Cuts at: 2549 2693 2549
Size: 144 5392
ApaLI G'TGCA_C
Cuts at: 2299 3593 4749 2299
Size: 1294 1156 3086
Fragments arranged by size:
3086 1294 1156
ApoI r'AATT_y
Cuts at: 1493 2845 3071 3223 5332 1493
Size: 1352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 2861 1870
Size: 73 690 228 4545
Fragments arranged by size:
4545 690 228 73
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2339 2857 4312 618
Size: 325 217 627 552 518 1455 1842
Fragments arranged by size:
1842 1455 627 552 518 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2091 2549 2693 2855 1391
Size: 629 71 458 144 162 4072
Fragments arranged by size:
4072 629 458 162 144 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2337 4147 136
Size: 107 122 71 631 605 665 1810 1525
Fragments arranged by size:
1810 1525 665 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2072 2953 2072
Size: 881 4655
Bme1580I G_kGCm'C
Cuts at: 1790 2303 2342 2383 2400 2549 2693 3597 4753
Size: 513 39 41 17 149 144 904 1156
Cuts at: 4753 1790
Size: 2573
Fragments arranged by size:
2573 1156 904 513 149 144 41 39
17
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 4627
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2376 2388 4196 1239
Size: 1137 12 1808 2579
Fragments arranged by size:
2579 1808 1137 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3661 4526 4824 3661
Size: 865 298 4373
Fragments arranged by size:
4373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 4199 882
Size: 617 1032 1668 2219
Fragments arranged by size:
2219 1668 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 2259 3327 493
Size: 711 1055 1068 2702
Fragments arranged by size:
2702 1068 1055 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2811 3343 3725 4360
Size: 53 83 186 936 1278 532 382 635
Cuts at: 4360 275
Size: 1451
Fragments arranged by size:
1451 1278 936 635 532 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2124 2724 2781 3969 4367 4641 4788 5199 2124
Size: 600 57 1188 398 274 147 411 2461
Fragments arranged by size:
2461 1188 600 411 398 274 147 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2801 2831 1178
Size: 30 165 30 1398 30 3883
Fragments arranged by size:
3883 1398 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2813 1186
Size: 859 768 3909
Fragments arranged by size:
3909 859 768
BseYI C'CCAG_C
Cuts at: 2026 2114 2343 2453 2518 2677 2686 4739 5235
Size: 88 229 110 65 159 9 2053 496
Cuts at: 5235 2026
Size: 2327
Fragments arranged by size:
2327 2053 496 229 159 110 88 65
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2161 2239 2428 1163
Size: 573 425 78 189 4271
Fragments arranged by size:
4271 573 425 189 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 2986 3747 4775 1540
Size: 275 186 124 861 761 1028 2301
Fragments arranged by size:
2301 1028 861 761 275 186 124
BsiHKAI G_wGCw'C
Cuts at: 2078 2303 3597 3682 4753 2078
Size: 225 1294 85 1071 2861
Fragments arranged by size:
2861 1294 1071 225 85
BsmI GAATG_Cn'
Cuts at: 3047 3140 3047
Size: 93 5443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2078 2091 2303 2342 2383 2400
Size: 399 230 58 13 212 39 41 17
Cuts at: 2400 2549 2693 2855 3597 3682 4753 1391
Size: 149 144 162 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 230 212 162 149
144 85 58 41 39 17 13
BspEI T'CCGG_A
Cuts at: 2724 2781 5199 2724
Size: 57 2418 3061
Fragments arranged by size:
3061 2418 57
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 2891 844
Size: 1883 164 3489
Fragments arranged by size:
3489 1883 164
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 2287 3423 1394
Size: 429 355 109 1136 3507
Fragments arranged by size:
3507 1136 429 355 109
BsrDI GCAATG_nn'
Cuts at: 65 1248 4031 4205 65
Size: 1183 2783 174 1396
Fragments arranged by size:
2783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2124 2473 4180 4367 1700
Size: 110 314 349 1707 187 2869
Fragments arranged by size:
2869 1707 349 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 2135 3596 4608 2135
Size: 1461 1012 3063
Fragments arranged by size:
3063 1461 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2253 2467 794
Size: 1459 214 3863
Fragments arranged by size:
3863 1459 214
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5536
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2707 2011
Size: 696 4840
BstYI r'GATC_y
Cuts at: 1972 2866 3233 3618 3635 5087 5173 5185 5531
Size: 894 367 385 17 1452 86 12 346
Cuts at: 5531 1972
Size: 1977
Fragments arranged by size:
1977 1452 894 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2032 2622 513
Size: 552 967 590 3427
Fragments arranged by size:
3427 967 590 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 3052 3846 3866 3052
Size: 794 20 4722
Fragments arranged by size:
4722 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DraIII CAC_nnn'GTG
Cuts at: 2193 2262 2193
Size: 69 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 2475 2553
Size: 54 1425 50 275 18 474 171 78
Cuts at: 2553 2697 2826 2983 3872 5501 8
Size: 144 129 157 889 1629 43
Fragments arranged by size:
1629 1425 889 474 275 171 157 144
129 78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3466 1353
Size: 2113 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4123 4497 4643 387
Size: 1064 138 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2376 2388 2601 3599 4196 4983 5251
Size: 393 744 12 213 998 597 787 268
Cuts at: 5251 1239
Size: 1524
Fragments arranged by size:
1524 998 787 744 597 393 268 213
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2769 2769
Size: 5536
EcoO109I rG'GnC_Cy
Cuts at: 2512 2545 2546 2606 2629 2690 2512
Size: 33 1 60 23 61 5358
Fragments arranged by size:
5358 61 60 33 23 1
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 4683 1678
Size: 3005 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2801 2833 4177 4209 4251
Size: 32 135 32 1229 32 1344 32 42
Cuts at: 4251 4283 1373
Size: 32 2626
Fragments arranged by size:
2626 1344 1229 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 2880 3126 669
Size: 988 281 436 506 246 3079
Fragments arranged by size:
3079 988 506 436 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4650 4834 1305
Size: 3345 184 2007
Fragments arranged by size:
3345 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2699 2828 10
Size: 54 1425 343 867 129 2718
Fragments arranged by size:
2718 1425 867 343 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3494 3853 4012 518
Size: 648 843 1485 359 159 2042
Fragments arranged by size:
2042 1485 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2169 2296 2328 2952 3629 4777 5022
Size: 428 135 127 32 624 677 1148 245
Cuts at: 5022 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 624 428 245 135 127
32
NaeI GCC'GGC
Cuts at: 2475 2475
Size: 5536
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4984
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NgoMIV G'CCGG_C
Cuts at: 2473 2473
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2894 4439 1132
Size: 97 1665 1545 2229
Fragments arranged by size:
2229 1665 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2542 2765 1348
Size: 1194 223 4119
Fragments arranged by size:
4119 1194 223
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 1108
Size: 1242 4294
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PmlI CAC'GTG
Cuts at: 2259 2259
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2512 2606 2629 2512
Size: 94 23 5419
Fragments arranged by size:
5419 94 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PspOMI G'GGCC_C
Cuts at: 2545 2689 2545
Size: 144 5392
PstI C_TGCA'G
Cuts at: 830 2174 2472 2888 830
Size: 1344 298 416 3478
Fragments arranged by size:
3478 1344 416 298
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
PvuII CAG'CTG
Cuts at: 2296 2296
Size: 5536
SacII CC_GC'GG
Cuts at: 2035 2035
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SanDI GG'GwC_CC
Cuts at: 2512 2606 2512
Size: 94 5442
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2170 2468 2884 4019 4700 4891 826
Size: 219 1125 298 416 1135 681 191 1471
Fragments arranged by size:
1471 1135 1125 681 416 298 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2635 2863 1872
Size: 763 228 4545
Fragments arranged by size:
4545 763 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3640 4541 4803 820
Size: 197 926 1697 901 262 1553
Fragments arranged by size:
1697 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 2423 1517
Size: 906 4630
StyI C'CwwG_G
Cuts at: 513 1065 2448 2975 513
Size: 552 1383 527 3074
Fragments arranged by size:
3074 1383 552 527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 3563 3580 3733 3918 4363 325
Size: 1255 777 1206 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1206 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3782 5494 96
Size: 274 80 33 51 1443 1805 1712 138
Fragments arranged by size:
1805 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5496 5528 5496
Size: 32 5504
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 2318 1316
Size: 912 90 4534
Fragments arranged by size:
4534 912 90
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2620 2662 2762 2446
Size: 174 42 100 5220
Fragments arranged by size:
5220 174 100 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2633 2861 1870
Size: 763 228 4545
Fragments arranged by size:
4545 763 228
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspXI PsrI RsrII SacI SapI SexAI
SfiI SfoI SrfI SwaI