(Linear) (Six-base) MAPSORT of: KIEE1768.seq Check: 1458 from: 1 to: 831 KIEE1768.orf With 182 enzymes: SgfI * July 15, 2010 18:47 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 596 831 Size: 596 235 AgeI A'CCGG_T Cuts at: 0 119 831 Size: 119 712 AlwNI CAG_nnn'CTG Cuts at: 0 345 735 831 Size: 345 390 96 Fragments arranged by size: 390 345 96 ApaI G_GGCC'C Cuts at: 0 544 688 831 Size: 544 144 143 Fragments arranged by size: 544 144 143 ApaLI G'TGCA_C Cuts at: 0 294 831 Size: 294 537 AvaI C'yCGr_G Cuts at: 0 628 831 Size: 628 203 BanI G'GyrC_C Cuts at: 0 334 831 Size: 334 497 BanII G_rGCy'C Cuts at: 0 15 86 544 688 831 Size: 15 71 458 144 143 Fragments arranged by size: 458 144 143 71 15 BglI GCCn_nnn'nGGC Cuts at: 0 332 831 Size: 332 499 BlpI GC'TnA_GC Cuts at: 0 67 831 Size: 67 764 Bme1580I G_kGCm'C Cuts at: 0 298 337 378 395 544 688 831 Size: 298 39 41 17 149 144 143 Fragments arranged by size: 298 149 144 143 41 39 17 BmgBI CAC'GTC Cuts at: 0 222 831 Size: 222 609 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 371 383 831 Size: 371 12 448 Fragments arranged by size: 448 371 12 BsaI GGTCTCn'nnnn_ Cuts at: 0 526 831 Size: 526 305 BsaAI yAC'GTr Cuts at: 0 254 831 Size: 254 577 BsaHI Gr'CG_yC Cuts at: 0 806 831 Size: 806 25 BsaWI w'CCGG_w Cuts at: 0 119 719 776 831 Size: 119 600 57 55 Fragments arranged by size: 600 119 57 55 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 796 826 831 Size: 796 30 5 Fragments arranged by size: 796 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 808 831 Size: 40 768 23 Fragments arranged by size: 768 40 23 BseYI C'CCAG_C Cuts at: 0 21 109 338 448 513 672 681 831 Size: 21 88 229 110 65 159 9 150 Fragments arranged by size: 229 159 150 110 88 65 21 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 156 234 423 831 Size: 156 78 189 408 Fragments arranged by size: 408 189 156 78 BsiEI CG_ry'CG Cuts at: 0 120 831 Size: 120 711 BsiHKAI G_wGCw'C Cuts at: 0 73 298 831 Size: 73 225 533 Fragments arranged by size: 533 225 73 Bsp1286I G_dGCh'C Cuts at: 0 15 73 86 298 337 378 395 544 Size: 15 58 13 212 39 41 17 149 Cuts at: 544 688 831 Size: 144 143 Fragments arranged by size: 212 149 144 143 58 41 39 17 15 13 BspEI T'CCGG_A Cuts at: 0 719 776 831 Size: 719 57 55 Fragments arranged by size: 719 57 55 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 722 831 Size: 722 109 BsrBI CCG'CTC Cuts at: 0 173 282 831 Size: 173 109 549 Fragments arranged by size: 549 173 109 BsrFI r'CCGG_y Cuts at: 0 119 468 831 Size: 119 349 363 Fragments arranged by size: 363 349 119 BssSI C'ACGA_G Cuts at: 0 130 831 Size: 130 701 BstAPI GCAn_nnn'nTGC Cuts at: 0 248 462 831 Size: 248 214 369 Fragments arranged by size: 369 248 214 BstXI CCAn_nnnn'nTGG Cuts at: 0 702 831 Size: 702 129 BtgI C'CryG_G Cuts at: 0 27 617 831 Size: 27 590 214 Fragments arranged by size: 590 214 27 DraIII CAC_nnn'GTG Cuts at: 0 188 257 831 Size: 188 69 574 Fragments arranged by size: 574 188 69 EaeI y'GGCC_r Cuts at: 0 299 470 548 692 821 831 Size: 299 171 78 144 129 10 Fragments arranged by size: 299 171 144 129 78 10 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 371 383 596 831 Size: 371 12 213 235 Fragments arranged by size: 371 235 213 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 764 831 Size: 764 67 EcoO109I rG'GnC_Cy Cuts at: 0 507 540 541 601 624 685 831 Size: 507 33 1 60 23 61 146 Fragments arranged by size: 507 146 61 60 33 23 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 796 828 831 Size: 796 32 3 Fragments arranged by size: 796 32 3 HincII GTy'rAC Cuts at: 0 369 831 Size: 369 462 MscI TGG'CCA Cuts at: 0 694 823 831 Size: 694 129 8 Fragments arranged by size: 694 129 8 MspA1I CmG'CkG Cuts at: 0 29 164 291 323 831 Size: 29 135 127 32 508 Fragments arranged by size: 508 135 127 32 29 NaeI GCC'GGC Cuts at: 0 470 831 Size: 470 361 NgoMIV G'CCGG_C Cuts at: 0 468 831 Size: 468 363 PasI CC'CwG_GG Cuts at: 0 537 760 831 Size: 537 223 71 Fragments arranged by size: 537 223 71 PflMI CCAn_nnn'nTGG Cuts at: 0 345 831 Size: 345 486 PmlI CAC'GTG Cuts at: 0 254 831 Size: 254 577 PpuMI rG'GwC_Cy Cuts at: 0 507 601 624 831 Size: 507 94 23 207 Fragments arranged by size: 507 207 94 23 PspOMI G'GGCC_C Cuts at: 0 540 684 831 Size: 540 144 147 Fragments arranged by size: 540 147 144 PstI C_TGCA'G Cuts at: 0 169 467 831 Size: 169 298 364 Fragments arranged by size: 364 298 169 PvuII CAG'CTG Cuts at: 0 291 831 Size: 291 540 SacII CC_GC'GG Cuts at: 0 30 831 Size: 30 801 SanDI GG'GwC_CC Cuts at: 0 507 601 831 Size: 507 94 230 Fragments arranged by size: 507 230 94 SfcI C'TryA_G Cuts at: 0 165 463 831 Size: 165 298 368 Fragments arranged by size: 368 298 165 SmaI CCC'GGG Cuts at: 0 630 831 Size: 630 201 StuI AGG'CCT Cuts at: 0 418 831 Size: 418 413 StyI C'CwwG_G Cuts at: 0 443 831 Size: 443 388 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 352 831 Size: 352 479 Tth111I GACn'n_nGTC Cuts at: 0 223 313 831 Size: 223 90 518 Fragments arranged by size: 518 223 90 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 441 615 657 757 831 Size: 441 174 42 100 74 Fragments arranged by size: 441 174 100 74 42 XmaI C'CCGG_G Cuts at: 0 628 831 Size: 628 203 Enzymes that do cut: AcuI AgeI AlwNI ApaI ApaLI AvaI BanI BanII BglI BlpI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstXI BtgI DraIII EaeI Eco57MI EcoNI EcoO109I Hin4I HincII MscI MspA1I NaeI NgoMIV PasI PflMI PmlI PpuMI PspOMI PstI PvuII SacII SanDI SfcI SmaI StuI StyI TaqII Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmnI ZraI