Restriction map for
pFN21AB4243
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BciVI BfrI BlnI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoRI EcoRV EcoT22I
HpaI KpnI MamI MfeI MroI
MunI NdeI NheI NotI NsiI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I RsrII SalI SbfI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 15 enzymes) #
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AccI 1357 GTCTAC GT^[AC][GT]AC
AviII 2201 TGCGCA TGC^GCA
BglII 1169 AGATCT A^GATCT
BsaWI 1374 ACCGGA [AT]^CCGG[AT]
BssHII 1256 GCGCGC G^CGCGC
FspI 2201 TGCGCA TGC^GCA
MluI 3047 ACGCGT A^CGCGT
NruI 3923 TCGCGA TCG^CGA
PaeR7I 3715 CTCGAG C^TCGAG
PvuI 3992 CGATCG CGAT^CG
SexAI 499 ACCTGGT A^CC[AT]GGT
SfiI 4282 GGCCAGGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 636 CGCCGGCG C[GA]^CCGG[CT]G
StuI 3914 AGGCCT AGG^CCT
XhoI 3715 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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Aor51HI 227 AGCGCT AGC^GCT
4061 AGCGCT
BamHI 275 GGATCC G^GATCC
525 GGATCC
BbeI 3 GGCGCC GGCGC^C
1780 GGCGCC
BbrPI 1023 CACGTG CAC^GTG
3529 CACGTG
BbvCI 270 GCTGAGG CCTCAGC(-5/-2)
285 GCTGAGG
BcgI 4059 CGAGCGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3707 GCATGGAACTCG
BclI 1850 TGATCA T^GATCA
3174 TGATCA
BfaI 530 CTAG C^TAG
3232 CTAG
BsmBI 235 GAGACG CGTCTC(1/5)
2742 GAGACG
DraIII 1381 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1405 CACAACGTG
Eco47III 227 AGCGCT AGC^GCT
4061 AGCGCT
FbaI 1850 TGATCA T^GATCA
3174 TGATCA
FseI 4052 GGCCGGCC GGCCGG^CC
4427 GGCCGGCC
HincII 217 GTCAAC GT[CT]^[GA]AC
1186 GTCAAC
HindII 217 GTCAAC GT[CT]^[GA]AC
1186 GTCAAC
HindIII 361 AAGCTT A^AGCTT
4494 AAGCTT
KasI 3 GGCGCC G^GCGCC
1780 GGCGCC
MaeI 530 CTAG C^TAG
3232 CTAG
MseI 1106 TTAA T^TAA
2750 TTAA
NarI 3 GGCGCC GG^CGCC
1780 GGCGCC
PflMI 1019 CCATCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3666 CCAGAAGCTGG
PmaCI 1023 CACGTG CAC^GTG
3529 CACGTG
PmlI 1023 CACGTG CAC^GTG
3529 CACGTG
PshAI 3126 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4329 GACGGGCGTC
SacI 1642 GAGCTC GAGCT^C
2266 GAGCTC
ScaI 4046 AGTACT AGT^ACT
4079 AGTACT
SfoI 3 GGCGCC GGC^GCC
1780 GGCGCC
SmaI 976 CCCGGG CCC^GGG
4154 CCCGGG
SphI 2768 GCATGC GCATG^C
3968 GCATGC
Tru9I 1106 TTAA T^TAA
2750 TTAA
Van91I 1019 CCATCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3666 CCAGAAGCTGG
XmaCI 976 CCCGGG C^CCGGG
4154 CCCGGG
XmaI 976 CCCGGG C^CCGGG
4154 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII