(Circular) (Six-base) MAPSORT of: pFN21AB4243.seq Check: 5551 from: 1 to: 9220 pFN21AB4243 9220 bp With 182 enzymes: SgfI * March 23, 2015 11:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2671 2780 2915 2671 Size: 109 135 8976 Fragments arranged by size: 8976 135 109 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7030 278 Size: 53 83 186 936 5494 2468 Fragments arranged by size: 5494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3363 6563 1937 Size: 1426 3200 4594 Fragments arranged by size: 4594 3200 1426 Acc65I G'GTAC_C Cuts at: 6541 6541 Size: 9220 AclI AA'CG_TT Cuts at: 1563 7347 7720 1563 Size: 5784 373 3063 Fragments arranged by size: 5784 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3749 4457 7283 8667 8935 1632 Size: 2117 708 2826 1384 268 1917 Fragments arranged by size: 2826 2117 1917 1384 708 268 AfeI AGC'GCT Cuts at: 2234 6068 2234 Size: 3834 5386 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9023 AflIII A'CryG_T Cuts at: 1203 2313 3131 5052 5701 5754 8119 9096 1203 Size: 1110 818 1921 649 53 2365 977 1327 Fragments arranged by size: 2365 1921 1327 1110 977 818 649 53 AgeI A'CCGG_T Cuts at: 8051 8051 Size: 9220 AhdI GACnn_n'nnGTC Cuts at: 7949 7949 Size: 9220 AleI CACnn'nnGTG Cuts at: 1166 2774 2909 3797 4968 5442 5639 6315 1166 Size: 1608 135 888 1171 474 197 676 4071 Fragments arranged by size: 4071 1608 1171 888 676 474 197 135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9220 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5708 5740 5708 Size: 32 9188 AlwNI CAG_nnn'CTG Cuts at: 2290 3053 3781 5677 6963 8535 2290 Size: 763 728 1896 1286 1572 2975 Fragments arranged by size: 2975 1896 1572 1286 763 728 ApaI G_GGCC'C Cuts at: 2024 2426 3213 3510 2024 Size: 402 787 297 7734 Fragments arranged by size: 7734 787 402 297 ApaLI G'TGCA_C Cuts at: 7277 8433 7277 Size: 1156 8064 ApoI r'AATT_y Cuts at: 1493 6529 6755 6907 9016 1493 Size: 5036 226 152 2109 1697 Fragments arranged by size: 5036 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7774 160 Size: 872 6742 1606 Fragments arranged by size: 6742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9220 AvaI C'yCGr_G Cuts at: 1870 1943 2837 2981 3037 5720 6159 6545 1870 Size: 73 894 144 56 2683 439 386 4545 Fragments arranged by size: 4545 2683 894 439 386 144 73 56 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6473 6506 6473 Size: 33 9187 BamHI G'GATC_C Cuts at: 2280 2530 6550 6917 2280 Size: 250 4020 367 4583 Fragments arranged by size: 4583 4020 367 250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 3785 4198 4333 6541 Size: 325 217 627 221 1777 413 135 2208 Cuts at: 6541 7996 618 Size: 1455 1842 Fragments arranged by size: 2208 1842 1777 1455 627 413 325 221 217 135 BanII G_rGCy'C Cuts at: 1391 2024 2100 2426 2578 2789 3158 3213 3336 Size: 633 76 326 152 211 369 55 123 Cuts at: 3336 3510 3617 3651 3942 4166 4275 4437 5873 Size: 174 107 34 291 224 109 162 1436 Cuts at: 5873 6539 1391 Size: 666 4072 Fragments arranged by size: 4072 1436 666 633 369 326 291 224 211 174 162 152 123 109 107 76 55 34 BbeI G_GCGC'C Cuts at: 2012 3789 2012 Size: 1777 7443 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3869 4597 5519 5967 6334 928 Size: 971 1970 728 922 448 367 3814 Fragments arranged by size: 3814 1970 971 922 728 448 367 BbvCI CC'TCA_GC Cuts at: 2276 2291 2276 Size: 15 9205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5701 5735 6053 6087 7411 7445 1903 Size: 34 3764 34 318 34 1324 34 3678 Fragments arranged by size: 3764 3678 1324 318 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7112 8322 7112 Size: 1210 8010 BclI T'GATC_A Cuts at: 1548 3855 5179 1548 Size: 2307 1324 5589 Fragments arranged by size: 5589 2307 1324 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4044 5463 6294 Size: 107 122 71 631 605 2372 1419 831 Cuts at: 6294 7831 136 Size: 1537 1525 Fragments arranged by size: 2372 1537 1525 1419 831 631 605 122 107 71 BglII A'GATC_T Cuts at: 3174 9215 3174 Size: 6041 3179 BlpI GC'TnA_GC Cuts at: 3290 3878 4778 6353 6637 3290 Size: 588 900 1575 284 5873 Fragments arranged by size: 5873 1575 900 588 284 Bme1580I G_kGCm'C Cuts at: 1790 2024 2426 3213 3510 4338 5358 5646 7281 Size: 234 402 787 297 828 1020 288 1635 Cuts at: 7281 8437 1790 Size: 1156 2573 Fragments arranged by size: 2573 1635 1156 1020 828 787 402 297 288 234 BmgBI CAC'GTC Cuts at: 1318 5704 5757 1318 Size: 4386 53 4781 Fragments arranged by size: 4781 4386 53 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6951 7909 442 Size: 416 53 83 399 24 5534 958 1753 Fragments arranged by size: 5534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3473 3631 4034 4349 4451 4752 4964 5174 Size: 2234 158 403 315 102 301 212 210 Cuts at: 5174 5281 5633 6386 6506 7880 1239 Size: 107 352 753 120 1374 2579 Fragments arranged by size: 2579 2234 1374 753 403 352 315 301 212 210 158 120 107 102 Bpu10I CC'TnA_GC Cuts at: 2276 2291 3152 2276 Size: 15 861 8344 Fragments arranged by size: 8344 861 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3974 4814 4832 5513 7345 8210 8508 3974 Size: 840 18 681 1832 865 298 4686 Fragments arranged by size: 4686 1832 865 840 681 298 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3455 3584 5078 5201 5945 7883 882 Size: 617 1956 129 1494 123 744 1938 2219 Fragments arranged by size: 2219 1956 1938 1494 744 617 129 123 BsaAI yAC'GTr Cuts at: 493 1204 2677 2803 3030 3715 5029 5536 7011 Size: 711 1473 126 227 685 1314 507 1475 Cuts at: 7011 493 Size: 2702 Fragments arranged by size: 2702 1475 1473 1314 711 685 507 227 126 BsaBI GATnn'nnATC Cuts at: 9214 9214 Size: 9220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2931 3786 5044 Size: 53 83 186 936 476 922 855 1258 Cuts at: 5044 5484 6339 7027 7409 8044 275 Size: 440 855 688 382 635 1451 Fragments arranged by size: 1451 1258 936 922 855 855 688 635 476 440 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3379 7653 8051 8325 8472 8883 3379 Size: 4274 398 274 147 411 3716 Fragments arranged by size: 4274 3716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4853 4883 5108 5138 5147 Size: 30 165 30 3450 30 225 30 9 Cuts at: 5147 5177 5570 5600 1178 Size: 30 393 30 4798 Fragments arranged by size: 4798 3450 393 225 165 30 30 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2647 3335 3641 4577 5171 1186 Size: 1461 688 306 936 594 5235 Fragments arranged by size: 5235 1461 936 688 594 306 BseYI C'CCAG_C Cuts at: 3215 3882 4100 4240 4542 5553 6426 8423 8919 Size: 667 218 140 302 1011 873 1997 496 Cuts at: 8919 3215 Size: 3516 Fragments arranged by size: 3516 1997 1011 873 667 496 302 218 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2106 2283 2493 3332 5267 5591 6464 Size: 573 370 177 210 839 1935 324 873 Cuts at: 6464 1163 Size: 3919 Fragments arranged by size: 3919 1935 873 839 573 370 324 210 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3189 5126 6000 6063 6434 6670 Size: 275 186 1188 1937 874 63 371 236 Cuts at: 6670 7431 8459 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1937 1188 1028 874 761 371 275 236 186 63 BsiHKAI G_wGCw'C Cuts at: 3387 3483 3651 4275 4818 5834 7281 7366 8437 Size: 96 168 624 543 1016 1447 85 1071 Cuts at: 8437 3387 Size: 4170 Fragments arranged by size: 4170 1447 1071 1016 624 543 168 96 85 BsmI GAATG_Cn' Cuts at: 6731 6824 6731 Size: 93 9127 BsmBI CGTCTCn'nnnn_ Cuts at: 2234 4741 2234 Size: 2507 6713 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2100 2426 2578 2789 3158 3213 Size: 399 234 76 326 152 211 369 55 Cuts at: 3213 3336 3387 3483 3510 3617 3651 3942 4166 Size: 123 51 96 27 107 34 291 224 Cuts at: 4166 4275 4338 4437 4818 5358 5646 5834 5873 Size: 109 63 99 381 540 288 188 39 Cuts at: 5873 6539 7281 7366 8437 1391 Size: 666 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 666 540 399 381 369 326 291 288 234 224 211 188 152 123 109 107 99 96 85 76 63 55 51 39 34 27 BspEI T'CCGG_A Cuts at: 8883 8883 Size: 9220 BspHI T'CATG_A Cuts at: 3858 5139 5544 7109 8839 3858 Size: 1281 405 1565 1730 4239 Fragments arranged by size: 4239 1730 1565 1281 405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2671 2780 2915 3576 4092 5411 6575 844 Size: 1827 109 135 661 516 1319 1164 3489 Fragments arranged by size: 3489 1827 1319 1164 661 516 135 109 BsrBI CCG'CTC Cuts at: 1394 1823 2446 3768 4825 5497 6370 7107 1394 Size: 429 623 1322 1057 672 873 737 3507 Fragments arranged by size: 3507 1322 1057 873 737 672 623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2551 4623 5058 7715 7889 65 Size: 1183 1303 2072 435 2657 174 1396 Fragments arranged by size: 2657 2072 1396 1303 1183 435 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2642 3325 5040 5420 5447 6058 6320 Size: 110 832 683 1715 380 27 611 262 Cuts at: 6320 6433 7864 8051 1700 Size: 113 1431 187 2869 Fragments arranged by size: 2869 1715 1431 832 683 611 380 262 187 113 110 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9220 BssHII G'CGCG_C Cuts at: 1925 3261 1925 Size: 1336 7884 BssSI C'ACGA_G Cuts at: 2190 2738 4215 5600 7280 8292 2190 Size: 548 1477 1385 1680 1012 3118 Fragments arranged by size: 3118 1680 1477 1385 1012 548 BstAPI GCAn_nnn'nTGC Cuts at: 794 3547 4689 5466 794 Size: 2753 1142 777 4548 Fragments arranged by size: 4548 2753 1142 777 BstBI TT'CG_AA Cuts at: 8024 8024 Size: 9220 BstEII G'GTnAC_C Cuts at: 1184 2177 3229 4060 1184 Size: 993 1052 831 6344 Fragments arranged by size: 6344 1052 993 831 BstXI CCAn_nnnn'nTGG Cuts at: 3935 5029 5251 3935 Size: 1094 222 7904 Fragments arranged by size: 7904 1094 222 BstYI r'GATC_y Cuts at: 1972 2280 2530 3049 3174 3924 4261 6097 6550 Size: 308 250 519 125 750 337 1836 453 Cuts at: 6550 6917 7302 7319 8771 8857 8869 9215 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1836 1452 750 519 453 385 367 346 337 308 250 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2172 2300 2781 2853 2991 Size: 552 939 45 123 128 481 72 138 Cuts at: 2991 3225 3266 3349 3372 3390 3438 3620 3670 Size: 234 41 83 23 18 48 182 50 Cuts at: 3670 3798 4599 5347 5619 5955 6438 513 Size: 128 801 748 272 336 483 3295 Fragments arranged by size: 3295 939 801 748 552 483 481 336 272 234 182 138 128 128 123 83 72 50 48 45 41 23 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4783 4936 5911 487 Size: 683 56 3557 153 975 3796 Fragments arranged by size: 3796 3557 975 683 153 56 BtsI GCAGTG_nn' Cuts at: 2277 2599 3320 4202 5011 5437 5449 6310 6322 Size: 322 721 882 809 426 12 861 12 Cuts at: 6322 6736 7530 7550 2277 Size: 414 794 20 3947 Fragments arranged by size: 3947 882 861 809 794 721 426 414 322 20 12 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3289 3324 4198 4233 529 Size: 35 2725 35 874 35 5516 Fragments arranged by size: 5516 2725 874 35 35 35 DraI TTT'AAA Cuts at: 6524 6524 Size: 9220 DraIII CAC_nnn'GTG Cuts at: 3391 3415 3391 Size: 24 9196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8227 809 Size: 507 6911 1802 Fragments arranged by size: 6911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 2778 2895 Size: 54 1425 50 275 18 798 150 117 Cuts at: 2895 2913 3186 3202 3255 3673 3958 4156 4438 Size: 18 273 16 53 418 285 198 282 Cuts at: 4438 4596 5253 5339 5530 6056 6060 6166 6206 Size: 158 657 86 191 526 4 106 40 Cuts at: 6206 6256 6286 6431 6435 6667 7556 9185 8 Size: 50 30 145 4 232 889 1629 43 Fragments arranged by size: 1629 1425 889 798 657 526 418 285 282 275 273 232 198 191 158 150 145 117 106 86 54 53 50 50 43 40 30 18 18 16 4 4 EagI C'GGCC_G Cuts at: 1537 1812 3186 6060 6431 6667 1537 Size: 275 1374 2874 371 236 4090 Fragments arranged by size: 4090 2874 1374 371 275 236 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 3659 4263 4460 4472 7150 1353 Size: 735 1571 604 197 12 2678 3423 Fragments arranged by size: 3423 2678 1571 735 604 197 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2687 3743 4826 5306 5963 7807 Size: 1064 138 1098 1056 1083 480 657 1844 Cuts at: 7807 8181 8327 387 Size: 374 146 1280 Fragments arranged by size: 1844 1280 1098 1083 1064 1056 657 480 374 146 138 EcoICRI GAG'CTC Cuts at: 3649 4273 3649 Size: 624 8596 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3473 3631 3749 4034 4349 4451 4457 Size: 393 1841 158 118 285 315 102 6 Cuts at: 4457 4752 4964 5174 5281 5633 6386 6506 7283 Size: 295 212 210 107 352 753 120 777 Cuts at: 7283 7880 8667 8935 1239 Size: 597 787 268 1524 Fragments arranged by size: 1841 1524 787 777 753 597 393 352 315 295 285 268 212 210 158 120 118 107 102 6 EcoNI CCTnn'n_nnAGG Cuts at: 2381 3452 5612 2381 Size: 1071 2160 5989 Fragments arranged by size: 5989 2160 1071 EcoO109I rG'GnC_Cy Cuts at: 2020 2021 2827 3209 3210 3250 3507 3690 3724 Size: 1 806 382 1 40 257 183 34 Cuts at: 3724 3778 4426 4732 5550 5605 2020 Size: 54 648 306 818 55 5635 Fragments arranged by size: 5635 818 806 648 382 306 257 183 55 54 40 34 1 1 EcoRI G'AATT_C Cuts at: 6529 6907 6529 Size: 378 8842 FseI GG_CCGG'CC Cuts at: 6062 6437 6062 Size: 375 8845 FspI TGC'GCA Cuts at: 4208 7726 4208 Size: 3518 5702 HaeII r_GCGC'y Cuts at: 1678 2012 2236 2643 3789 6070 8367 1678 Size: 334 224 407 1146 2281 2297 2531 Fragments arranged by size: 2531 2297 2281 1146 407 334 224 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3041 3073 3569 3601 4853 Size: 32 135 32 1469 32 496 32 1252 Cuts at: 4853 4885 5108 5140 5147 5179 5263 5295 6155 Size: 32 223 32 7 32 84 32 860 Cuts at: 6155 6187 7861 7893 7935 7967 1373 Size: 32 1674 32 42 32 2626 Fragments arranged by size: 2626 1674 1469 1252 860 496 223 135 84 42 32 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2224 3193 6564 6810 669 Size: 988 281 286 969 3371 246 3079 Fragments arranged by size: 3371 3079 988 969 286 281 246 HindIII A'AGCT_T Cuts at: 748 2366 6499 748 Size: 1618 4133 3469 Fragments arranged by size: 4133 3469 1618 HpaI GTT'AAC Cuts at: 6810 6810 Size: 9220 KasI G'GCGC_C Cuts at: 2008 3785 2008 Size: 1777 7443 KpnI G_GTAC'C Cuts at: 6545 6545 Size: 9220 MfeI C'AATT_G Cuts at: 6819 6819 Size: 9220 MluI A'CGCG_T Cuts at: 5052 9096 5052 Size: 4044 5176 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2056 2840 3567 4932 8334 8518 1305 Size: 751 784 727 1365 3402 184 2007 Fragments arranged by size: 3402 2007 1365 784 751 727 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 2915 5255 5532 6168 Size: 54 1425 343 798 285 2340 277 636 Cuts at: 6168 6258 6288 10 Size: 90 30 2942 Fragments arranged by size: 2942 2340 1425 798 636 343 285 277 90 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2417 2774 2852 2909 3308 3797 4139 Size: 648 1251 357 78 57 399 489 342 Cuts at: 4139 4193 4446 4968 5138 5289 5442 5639 5711 Size: 54 253 522 170 151 153 197 72 Cuts at: 5711 6315 7178 7537 7696 518 Size: 604 863 359 159 2042 Fragments arranged by size: 2042 1251 863 648 604 522 489 399 359 357 342 253 197 170 159 153 151 78 72 57 54 MspA1I CmG'CkG Cuts at: 1606 2290 2302 2392 2481 2563 3268 3351 3622 Size: 684 12 90 89 82 705 83 271 Cuts at: 3622 3882 4240 4327 4525 5077 5122 5944 6430 Size: 260 358 87 198 552 45 822 486 Cuts at: 6430 6636 7313 8461 8706 1606 Size: 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 822 705 684 677 552 486 358 271 260 245 206 198 90 89 87 83 82 45 12 NaeI GCC'GGC Cuts at: 2644 5422 6060 6322 6435 2644 Size: 2778 638 262 113 5429 Fragments arranged by size: 5429 2778 638 262 113 NarI GG'CG_CC Cuts at: 2009 3786 2009 Size: 1777 7443 NcoI C'CATG_G Cuts at: 513 1065 2004 2049 2991 3372 3670 5347 6438 Size: 552 939 45 942 381 298 1677 1091 Cuts at: 6438 513 Size: 3295 Fragments arranged by size: 3295 1677 1091 942 939 552 381 298 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 9220 NgoMIV G'CCGG_C Cuts at: 2642 5420 6058 6320 6433 2642 Size: 2778 638 262 113 5429 Fragments arranged by size: 5429 2778 638 262 113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9220 NotI GC'GGCC_GC Cuts at: 6667 6667 Size: 9220 NruI TCG'CGA Cuts at: 5930 5930 Size: 9220 NspI r_CATG'y Cuts at: 1132 1229 2851 4777 5977 6121 6578 8123 1132 Size: 97 1622 1926 1200 144 457 1545 2229 Fragments arranged by size: 2229 1926 1622 1545 1200 457 144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9220 PciI A'CATG_T Cuts at: 8119 8119 Size: 9220 PflMI CCAn_nnn'nTGG Cuts at: 1108 3030 5677 1108 Size: 1922 2647 4651 Fragments arranged by size: 4651 2647 1922 PfoI T'CCnGG_A Cuts at: 3608 3657 4278 6173 3608 Size: 49 621 1895 6655 Fragments arranged by size: 6655 1895 621 49 PmeI GTTT'AAAC Cuts at: 6524 6524 Size: 9220 PmlI CAC'GTG Cuts at: 3030 5536 3030 Size: 2506 6714 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6449 6481 7289 7321 8802 8834 6449 Size: 32 808 32 1481 32 6835 Fragments arranged by size: 6835 1481 808 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2827 3690 3724 4732 5550 2827 Size: 863 34 1008 818 6497 Fragments arranged by size: 6497 1008 863 818 34 PshAI GACnn'nnGTC Cuts at: 1613 5135 6338 1613 Size: 3522 1203 4495 Fragments arranged by size: 4495 3522 1203 PsiI TTA'TAA Cuts at: 6790 6790 Size: 9220 PspOMI G'GGCC_C Cuts at: 2020 2422 3209 3506 2020 Size: 402 787 297 7734 Fragments arranged by size: 7734 787 402 297 PspXI vC'TCGA_Gb Cuts at: 5720 5720 Size: 9220 PstI C_TGCA'G Cuts at: 830 2583 3822 5444 6317 6572 830 Size: 1753 1239 1622 873 255 3478 Fragments arranged by size: 3478 1753 1622 1239 873 255 PvuI CG_AT'CG Cuts at: 2001 6000 2001 Size: 3999 5221 PvuII CAG'CTG Cuts at: 2392 2563 3882 4327 4525 2392 Size: 171 1319 445 198 7087 Fragments arranged by size: 7087 1319 445 198 171 SacI G_AGCT'C Cuts at: 3651 4275 3651 Size: 624 8596 SacII CC_GC'GG Cuts at: 2303 3269 3352 3623 2303 Size: 966 83 271 7900 Fragments arranged by size: 7900 966 271 83 SalI G'TCGA_C Cuts at: 1936 6562 1936 Size: 4626 4594 SapI GCTCTTCn'nnn_ Cuts at: 2088 4263 4472 2088 Size: 2175 209 6836 Fragments arranged by size: 6836 2175 209 SbfI CC_TGCA'GG Cuts at: 6572 6572 Size: 9220 ScaI AGT'ACT Cuts at: 6053 6086 7468 9180 6053 Size: 33 1382 1712 6093 Fragments arranged by size: 6093 1712 1382 33 SexAI A'CCwGG_T Cuts at: 2504 2504 Size: 9220 SfcI C'TryA_G Cuts at: 826 1045 2579 3818 4295 5440 5905 6313 6568 Size: 219 1534 1239 477 1145 465 408 255 Cuts at: 6568 7703 8384 8575 826 Size: 1135 681 191 1471 Fragments arranged by size: 1534 1471 1239 1145 1135 681 477 465 408 255 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 6294 6294 Size: 9220 SfoI GGC'GCC Cuts at: 2010 3787 2010 Size: 1777 7443 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9220 SgrAI Cr'CCGG_yG Cuts at: 1700 2642 1700 Size: 942 8278 SmaI CCC'GGG Cuts at: 1872 2983 6161 6547 1872 Size: 1111 3178 386 4545 Fragments arranged by size: 4545 3178 1111 386 SmlI C'TyrA_G Cuts at: 820 1017 1943 3989 4829 4847 5492 5720 7324 Size: 197 926 2046 840 18 645 228 1604 Cuts at: 7324 8225 8487 820 Size: 901 262 1553 Fragments arranged by size: 2046 1604 1553 926 901 840 645 262 228 197 18 SnaBI TAC'GTA Cuts at: 493 7011 493 Size: 6518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9220 SphI G_CATG'C Cuts at: 1132 4777 5977 6578 1132 Size: 3645 1200 601 3774 Fragments arranged by size: 3774 3645 1200 601 SspI AAT'ATT Cuts at: 5 52 7144 5 Size: 47 7092 2081 Fragments arranged by size: 7092 2081 47 StuI AGG'CCT Cuts at: 1517 5921 1517 Size: 4404 4816 StyI C'CwwG_G Cuts at: 513 1065 2004 2049 2121 2991 3372 3670 4728 Size: 552 939 45 72 870 381 298 1058 Cuts at: 4728 5347 5745 6438 6659 513 Size: 619 398 693 221 3074 Fragments arranged by size: 3074 1058 939 870 693 619 552 398 381 298 221 72 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3169 3925 4390 4567 7247 7264 7417 Size: 1255 1589 756 465 177 2680 17 153 Cuts at: 7417 7602 8047 325 Size: 185 445 1498 Fragments arranged by size: 2680 1589 1498 1255 756 465 445 185 177 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3930 5586 6051 Size: 274 80 33 51 1443 1953 1656 465 Cuts at: 6051 6084 7466 9178 96 Size: 33 1382 1712 138 Fragments arranged by size: 1953 1712 1656 1443 1382 465 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4365 4369 4397 4401 5161 5193 9180 9212 4365 Size: 4 28 4 760 32 3987 32 4373 Fragments arranged by size: 4373 3987 760 32 32 28 4 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9220 XbaI T'CTAG_A Cuts at: 6556 6556 Size: 9220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2196 2339 2396 2416 2805 2923 3223 4403 4735 Size: 143 57 20 389 118 300 1180 332 Cuts at: 4735 4804 5690 5860 2196 Size: 69 886 170 5556 Fragments arranged by size: 5556 1180 886 389 332 300 170 143 118 69 57 20 XhoI C'TCGA_G Cuts at: 1943 5720 1943 Size: 3777 5443 XmaI C'CCGG_G Cuts at: 1870 2981 6159 6545 1870 Size: 1111 3178 386 4545 Fragments arranged by size: 4545 3178 1111 386 XmnI GAAnn'nnTTC Cuts at: 7349 7349 Size: 9220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7028 276 Size: 53 83 186 936 5494 2468 Fragments arranged by size: 5494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FspAI NsiI PacI PsrI RsrII SanDI SrfI SwaI