################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BbvCI BfrI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 399 GTAC GT^AC AflIII 1236 ACACGT A^C[GA][CT]GT AhdI 1235 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1235 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1091 CTCGGG C^[CT]CG[GA]G AvaII 1242 GGTCC G^G[AT]CC BamHI 305 GGATCC G^GATCC BanI 1260 GGTGCC G^G[CT][GA]CC BbrPI 1237 CACGTG CAC^GTG BcgI 1439 CGATCTTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 911 GGATAC GTATCC(6/5) BfaI 205 CTAG C^TAG BsaAI 1237 CACGTG [CT]AC^GT[GA] BsaI 95 GGTCTC GGTCTC(1/5) BsgI 554 CTGCAC GTGCAG(16/14) BsmBI 538 GAGACG CGTCTC(1/5) BsoBI 1091 CTCGGG C^[CT]CG[GA]G BspHI 689 TCATGA T^CATGA BspMI 1002 GCAGGT ACCTGC(4/8) BstEII 1137 GGTGACC G^GT[ACGT]ACC BstPI 1137 GGTGACC G^GT[ACGT]ACC DraII 318 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 961 CCGCGG C^C[GA][CT]GG Eam1105I 1235 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 25 GAAGAG CTCTTC(1/4) EcoO109I 318 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 1137 GGTGACC G^GT[ACGT]ACC FauI 960 CCCGC CCCGC(4/6) Ksp632I 25 GAAGAG CTCTTC(1/4) KspI 961 CCGCGG CCGC^GG MaeI 205 CTAG C^TAG PmaCI 1237 CACGTG CAC^GTG PmlI 1237 CACGTG CAC^GTG PstI 1066 CTGCAG CTGCA^G RcaI 689 TCATGA T^CATGA RsaI 399 GTAC GT^AC SacII 961 CCGCGG CCGC^GG SapI 25 GAAGAGC GCTCTTC(1/4) SfcI 1066 CTGCAG C^T[GA][CT]AG SmlI 1396 CTCAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 962 CGCG CG^CG 1685 CGCG AlwNI 889 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1003 CAGGTGCTG ApaI 318 GGGCCC GGGCC^C 803 GGGCCC BalI 773 TGGCCA TGG^CCA 1402 TGGCCA BclI 506 TGATCA T^GATCA 662 TGATCA BlpI 337 GCTGAGC GC^T[ACGT]AGC 1507 GCTGAGC Bpu1102I 337 GCTGAGC GC^T[ACGT]AGC 1507 GCTGAGC BsrI 884 ACTGG ACTGG(1/-1) 435 CCAGT BstUI 962 CGCG CG^CG 1685 CGCG Cac8I 1263 GCCTGC GC[ACGT]^[ACGT]GC 1352 GCTTGC CelII 337 GCTGAGC GC^T[ACGT]AGC 1507 GCTGAGC DraIII 262 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG 777 CACTGAGTG EcoT14I 412 CCTTGG C^C[AT][AT]GG 1142 CCTTGG FbaI 506 TGATCA T^GATCA 662 TGATCA MluNI 773 TGGCCA TGG^CCA 1402 TGGCCA MscI 773 TGGCCA TGG^CCA 1402 TGGCCA MslI 564 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1254 CATGGAGGTG MvnI 962 CGCG CG^CG 1685 CGCG NspI 388 ACATGC [GA]CATG^[CT] 460 ACATGC PflMI 776 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1340 CCATCATCTGG SacI 28 GAGCTC GAGCT^C 1510 GAGCTC StyI 412 CCTTGG C^C[AT][AT]GG 1142 CCTTGG Van91I 776 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1340 CCATCATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AluI AlwI AspHI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstXI BstYI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp45I TspRI XcmI XhoII