Restriction map for
pFN21AE6795
(Circular) (Six-base) MAPSORT of: pFN21AE6795.seq Check: 2839 from: 1 to: 6487
pFN21AE6795 6487 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2998 2998
Size: 6487
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4297 278
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3830 1937
Size: 1893 4594
Acc65I G'GTAC_C
Cuts at: 3808 3808
Size: 6487
AclI AA'CG_TT
Cuts at: 1563 4614 4987 1563
Size: 3051 373 3063
Fragments arranged by size:
3063 3051 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2590 3220 3399 4550 5934 6202 1632
Size: 958 630 179 1151 1384 268 1917
Fragments arranged by size:
1917 1384 1151 958 630 268 179
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6290
AflIII A'CryG_T
Cuts at: 1203 3241 5386 6363 1203
Size: 2038 2145 977 1327
Fragments arranged by size:
2145 2038 1327 977
AgeI A'CCGG_T
Cuts at: 5318 5318
Size: 6487
AhdI GACnn_n'nnGTC
Cuts at: 3245 5216 3245
Size: 1971 4516
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6487
AlwNI CAG_nnn'CTG
Cuts at: 2899 3013 4230 5802 2899
Size: 114 1217 1572 3584
Fragments arranged by size:
3584 1572 1217 114
ApaI G_GGCC'C
Cuts at: 2327 2812 2327
Size: 485 6002
ApaLI G'TGCA_C
Cuts at: 4544 5700 4544
Size: 1156 5331
ApoI r'AATT_y
Cuts at: 1493 3796 4022 4174 6283 1493
Size: 2303 226 152 2109 1697
Fragments arranged by size:
2303 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5041 160
Size: 872 4009 1606
Fragments arranged by size:
4009 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
AvaI C'yCGr_G
Cuts at: 1870 1943 3096 3812 1870
Size: 73 1153 716 4545
Fragments arranged by size:
4545 1153 716 73
BamHI G'GATC_C
Cuts at: 2310 3817 4184 2310
Size: 1507 367 4613
Fragments arranged by size:
4613 1507 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3265 3808 5263 618
Size: 325 217 627 1478 543 1455 1842
Fragments arranged by size:
1842 1478 1455 627 543 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2327 2586 2812 3519 3806 1391
Size: 646 290 259 226 707 287 4072
Fragments arranged by size:
4072 707 646 290 287 259 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2873 3047 3764 928
Size: 971 974 174 717 3651
Fragments arranged by size:
3651 974 971 717 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3433 3467 4678 4712 1903
Size: 34 1496 34 1211 34 3678
Fragments arranged by size:
3678 1496 1211 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2910 4379 5589 2910
Size: 1469 1210 3808
Fragments arranged by size:
3808 1469 1210
BclI T'GATC_A
Cuts at: 1548 2511 2667 1548
Size: 963 156 5368
Fragments arranged by size:
5368 963 156
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3274 3572 3656
Size: 107 122 71 631 605 1602 298 84
Cuts at: 3656 5098 136
Size: 1442 1525
Fragments arranged by size:
1602 1525 1442 631 605 298 122 107
84 71
BglII A'GATC_T
Cuts at: 6482 6482
Size: 6487
BlpI GC'TnA_GC
Cuts at: 2343 3513 3904 2343
Size: 1170 391 4926
Fragments arranged by size:
4926 1170 391
Bme1580I G_kGCm'C
Cuts at: 1790 2327 2812 4548 5704 1790
Size: 537 485 1736 1156 2573
Fragments arranged by size:
2573 1736 1156 537 485
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6487
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4218 5176 442
Size: 416 53 83 399 24 2801 958 1753
Fragments arranged by size:
2801 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 3281 3597 5147 1239
Size: 887 1155 316 1550 2579
Fragments arranged by size:
2579 1550 1155 887 316
Bpu10I CC'TnA_GC
Cuts at: 3533 3533
Size: 6487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3386 4612 5477 5775 3386
Size: 1226 865 298 4098
Fragments arranged by size:
4098 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2106 5150 882
Size: 617 607 3044 2219
Fragments arranged by size:
3044 2219 617 607
BsaAI yAC'GTr
Cuts at: 493 1204 3244 4278 493
Size: 711 2040 1034 2702
Fragments arranged by size:
2702 2040 1034 711
BsaBI GATnn'nnATC
Cuts at: 6481 6481
Size: 6487
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4294 4676 5311 275
Size: 53 83 186 936 2761 382 635 1451
Fragments arranged by size:
2761 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4920 5318 5592 5739 6150 4920
Size: 398 274 147 411 5257
Fragments arranged by size:
5257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 1178
Size: 30 165 30 696 30 5536
Fragments arranged by size:
5536 696 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 3284 3326 3509 3661 3715 1186
Size: 844 1254 42 183 152 54 3958
Fragments arranged by size:
3958 1254 844 183 152 54 42
BseYI C'CCAG_C
Cuts at: 2231 2659 3217 3251 5690 6186 2231
Size: 428 558 34 2439 496 2532
Fragments arranged by size:
2532 2439 558 496 428 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2544 1163
Size: 573 808 5106
Fragments arranged by size:
5106 808 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3937 4698 5726 1540
Size: 275 186 1936 761 1028 2301
Fragments arranged by size:
2301 1936 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2805 3456 3519 3534 4548 4633 5704 2037
Size: 768 651 63 15 1014 85 1071 2820
Fragments arranged by size:
2820 1071 1014 768 651 85 63 15
BsmI GAATG_Cn'
Cuts at: 3998 4091 3998
Size: 93 6394
BsmBI CGTCTCn'nnnn_
Cuts at: 2537 2537
Size: 6487
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2327 2586 2805 2812 3456 3519
Size: 399 247 290 259 219 7 644 63
Cuts at: 3519 3534 3806 4548 4633 5704 1391
Size: 15 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 399 290 272 259
247 219 85 63 15 7
BspEI T'CCGG_A
Cuts at: 6150 6150
Size: 6487
BspHI T'CATG_A
Cuts at: 2694 4376 6106 2694
Size: 1682 1730 3075
Fragments arranged by size:
3075 1730 1682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2998 3842 844
Size: 2154 844 3489
Fragments arranged by size:
3489 2154 844
BsrBI CCG'CTC
Cuts at: 1394 1823 4374 1394
Size: 429 2551 3507
Fragments arranged by size:
3507 2551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4982 5156 65
Size: 1183 3734 174 1396
Fragments arranged by size:
3734 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5131 5318 1700
Size: 110 3321 187 2869
Fragments arranged by size:
3321 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6487
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6487
BssSI C'ACGA_G
Cuts at: 4547 5559 4547
Size: 1012 5475
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6487
BstBI TT'CG_AA
Cuts at: 5291 5291
Size: 6487
BstEII G'GTnAC_C
Cuts at: 1184 3142 1184
Size: 1958 4529
BstXI CCAn_nnnn'nTGG
Cuts at: 2097 2170 2824 3259 3305 2097
Size: 73 654 435 46 5279
Fragments arranged by size:
5279 654 435 73 46
BstYI r'GATC_y
Cuts at: 1972 2039 2310 2481 3817 4184 4569 4586 6038
Size: 67 271 171 1336 367 385 17 1452
Cuts at: 6038 6124 6136 6482 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1336 385 367 346 271 171
86 67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2966 513
Size: 552 939 962 4034
Fragments arranged by size:
4034 962 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5748
Fragments arranged by size:
5748 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 2556 2654 2830 4003 4797 4817 2325
Size: 231 98 176 1173 794 20 3995
Fragments arranged by size:
3995 1173 794 231 176 98 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2459 2494 529
Size: 35 1895 35 4522
Fragments arranged by size:
4522 1895 35 35
DraI TTT'AAA
Cuts at: 3791 3791
Size: 6487
DraIII CAC_nnn'GTG
Cuts at: 2272 2787 2272
Size: 515 5972
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5494 809
Size: 507 4178 1802
Fragments arranged by size:
4178 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2778 3407 3420
Size: 54 1425 50 275 18 948 629 13
Cuts at: 3420 3477 3934 4823 6452 8
Size: 57 457 889 1629 43
Fragments arranged by size:
1629 1425 948 889 629 457 275 57
54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3934 1537
Size: 275 2122 4090
Fragments arranged by size:
4090 2122 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 4417 1353
Size: 672 2392 3423
Fragments arranged by size:
3423 2392 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5074 5448 5594 387
Size: 1064 138 3485 374 146 1280
Fragments arranged by size:
3485 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2035 3517 2035
Size: 1482 5005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2590 3220 3281 3399 3597 4550
Size: 393 494 464 630 61 118 198 953
Cuts at: 4550 5147 5934 6202 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 953 787 630 597 494 464 393
268 198 118 61
EcoO109I rG'GnC_Cy
Cuts at: 2324 2324
Size: 6487
EcoRI G'AATT_C
Cuts at: 3796 4174 3796
Size: 378 6109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 6487
FspI TGC'GCA
Cuts at: 4993 4993
Size: 6487
HaeII r_GCGC'y
Cuts at: 1678 5634 1678
Size: 3956 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2496 2528 2581
Size: 32 135 32 452 32 440 32 53
Cuts at: 2581 2613 3231 3263 3419 3451 5128 5160 5202
Size: 32 618 32 156 32 1677 32 42
Cuts at: 5202 5234 1373
Size: 32 2626
Fragments arranged by size:
2626 1677 618 452 440 156 135 53
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3831 4077 669
Size: 988 281 1893 246 3079
Fragments arranged by size:
3079 1893 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6487
HpaI GTT'AAC
Cuts at: 4077 4077
Size: 6487
KpnI G_GTAC'C
Cuts at: 3812 3812
Size: 6487
MfeI C'AATT_G
Cuts at: 4086 4086
Size: 6487
MluI A'CGCG_T
Cuts at: 6363 6363
Size: 6487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5601 5785 1305
Size: 4296 184 2007
Fragments arranged by size:
4296 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2780 3409 10
Size: 54 1425 343 948 629 3088
Fragments arranged by size:
3088 1425 948 629 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2573 3263 4445 4804 4963 518
Size: 648 1407 690 1182 359 159 2042
Fragments arranged by size:
2042 1407 1182 690 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2307 2350 2896 2968 3076 3512 3903 4580
Size: 701 43 546 72 108 436 391 677
Cuts at: 4580 5728 5973 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 701 677 546 436 391 245
108 72 43
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4996
Fragments arranged by size:
4996 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6487
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6487
NotI GC'GGCC_GC
Cuts at: 3934 3934
Size: 6487
NspI r_CATG'y
Cuts at: 1132 1229 2397 2469 3845 5390 1132
Size: 97 1168 72 1376 1545 2229
Fragments arranged by size:
2229 1545 1376 1168 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6487
PciI A'CATG_T
Cuts at: 5386 5386
Size: 6487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2787 3351 1108
Size: 1679 564 4244
Fragments arranged by size:
4244 1679 564
PfoI T'CCnGG_A
Cuts at: 2054 2169 2955 3108 2054
Size: 115 786 153 5433
Fragments arranged by size:
5433 786 153 115
PmeI GTTT'AAAC
Cuts at: 3791 3791
Size: 6487
PmlI CAC'GTG
Cuts at: 3244 3244
Size: 6487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 4556 4588 6069 6101 3710
Size: 32 814 32 1481 32 4096
Fragments arranged by size:
4096 1481 814 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6487
PsiI TTA'TAA
Cuts at: 4057 4057
Size: 6487
PspOMI G'GGCC_C
Cuts at: 2323 2808 2323
Size: 485 6002
PstI C_TGCA'G
Cuts at: 830 3075 3839 830
Size: 2245 764 3478
Fragments arranged by size:
3478 2245 764
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6487
PvuII CAG'CTG
Cuts at: 2307 2350 2896 3076 2307
Size: 43 546 180 5718
Fragments arranged by size:
5718 546 180 43
SacI G_AGCT'C
Cuts at: 2037 3519 2037
Size: 1482 5005
SacII CC_GC'GG
Cuts at: 2969 2969
Size: 6487
SalI G'TCGA_C
Cuts at: 1936 3829 1936
Size: 1893 4594
SapI GCTCTTCn'nnn_
Cuts at: 2025 2025
Size: 6487
SbfI CC_TGCA'GG
Cuts at: 3839 3839
Size: 6487
ScaI AGT'ACT
Cuts at: 4735 6447 4735
Size: 1712 4775
SfcI C'TryA_G
Cuts at: 826 1045 3071 3835 4970 5651 5842 826
Size: 219 2026 764 1135 681 191 1471
Fragments arranged by size:
2026 1471 1135 764 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6487
SmaI CCC'GGG
Cuts at: 1872 3814 1872
Size: 1942 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3401 4591 5492 5754 820
Size: 197 926 1458 1190 901 262 1553
Fragments arranged by size:
1553 1458 1190 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4278 493
Size: 3785 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6487
SphI G_CATG'C
Cuts at: 1132 3845 1132
Size: 2713 3774
SspI AAT'ATT
Cuts at: 5 52 4411 5
Size: 47 4359 2081
Fragments arranged by size:
4359 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6487
StyI C'CwwG_G
Cuts at: 513 1065 2004 2417 3147 3926 513
Size: 552 939 413 730 779 3074
Fragments arranged by size:
3074 939 779 730 552 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2389 2466 2786 2900 4514 4531 4684
Size: 1255 809 77 320 114 1614 17 153
Cuts at: 4684 4869 5314 325
Size: 185 445 1498
Fragments arranged by size:
1614 1498 1255 809 445 320 185 153
114 77 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4733 6445 96
Size: 274 80 33 51 1443 2756 1712 138
Fragments arranged by size:
2756 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6447 6479 6447
Size: 32 6455
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6487
XbaI T'CTAG_A
Cuts at: 3823 3823
Size: 6487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2375 3445 2311
Size: 64 1070 5353
Fragments arranged by size:
5353 1070 64
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6487
XmaI C'CCGG_G
Cuts at: 1870 3812 1870
Size: 1942 4545
XmnI GAAnn'nnTTC
Cuts at: 4616 4616
Size: 6487
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4295 276
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI