(Linear) (Six-base) MAPSORT of: KIEE6795.seq Check: 440 from: 1 to: 1782 KIEE6795.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 993 1782 Size: 993 789 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 585 1215 1394 1782 Size: 585 630 179 388 Fragments arranged by size: 630 585 388 179 AflIII A'CryG_T Cuts at: 0 1236 1782 Size: 1236 546 AhdI GACnn_n'nnGTC Cuts at: 0 1240 1782 Size: 1240 542 AlwNI CAG_nnn'CTG Cuts at: 0 894 1008 1782 Size: 894 114 774 Fragments arranged by size: 894 774 114 ApaI G_GGCC'C Cuts at: 0 322 807 1782 Size: 322 485 975 Fragments arranged by size: 975 485 322 AvaI C'yCGr_G Cuts at: 0 1091 1782 Size: 1091 691 BamHI G'GATC_C Cuts at: 0 305 1782 Size: 305 1477 BanI G'GyrC_C Cuts at: 0 1260 1782 Size: 1260 522 BanII G_rGCy'C Cuts at: 0 32 322 581 807 1514 1782 Size: 32 290 259 226 707 268 Fragments arranged by size: 707 290 268 259 226 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 868 1042 1759 1782 Size: 868 174 717 23 Fragments arranged by size: 868 717 174 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1428 1462 1782 Size: 1428 34 320 Fragments arranged by size: 1428 320 34 BciVI GTATCCnnnnn_n' Cuts at: 0 905 1782 Size: 905 877 BclI T'GATC_A Cuts at: 0 506 662 1782 Size: 506 156 1120 Fragments arranged by size: 1120 506 156 BglI GCCn_nnn'nGGC Cuts at: 0 1269 1567 1651 1782 Size: 1269 298 84 131 Fragments arranged by size: 1269 298 131 84 BlpI GC'TnA_GC Cuts at: 0 338 1508 1782 Size: 338 1170 274 Fragments arranged by size: 1170 338 274 Bme1580I G_kGCm'C Cuts at: 0 322 807 1782 Size: 322 485 975 Fragments arranged by size: 975 485 322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1276 1592 1782 Size: 121 1155 316 190 Fragments arranged by size: 1155 316 190 121 Bpu10I CC'TnA_GC Cuts at: 0 1528 1782 Size: 1528 254 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1381 1782 Size: 1381 401 BsaI GGTCTCn'nnnn_ Cuts at: 0 101 1782 Size: 101 1681 BsaAI yAC'GTr Cuts at: 0 1239 1782 Size: 1239 543 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 94 124 1782 Size: 94 30 1658 Fragments arranged by size: 1658 94 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 1279 1321 1504 1656 1710 1782 Size: 25 1254 42 183 152 54 72 Fragments arranged by size: 1254 183 152 72 54 42 25 BseYI C'CCAG_C Cuts at: 0 226 654 1212 1246 1782 Size: 226 428 558 34 536 Fragments arranged by size: 558 536 428 226 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 539 1782 Size: 539 1243 BsiHKAI G_wGCw'C Cuts at: 0 32 800 1451 1514 1529 1782 Size: 32 768 651 63 15 253 Fragments arranged by size: 768 651 253 63 32 15 BsmBI CGTCTCn'nnnn_ Cuts at: 0 532 1782 Size: 532 1250 Bsp1286I G_dGCh'C Cuts at: 0 32 322 581 800 807 1451 1514 1529 Size: 32 290 259 219 7 644 63 15 Cuts at: 1529 1782 Size: 253 Fragments arranged by size: 644 290 259 253 219 63 32 15 7 BspHI T'CATG_A Cuts at: 0 689 1782 Size: 689 1093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 993 1782 Size: 993 789 BstEII G'GTnAC_C Cuts at: 0 1137 1782 Size: 1137 645 BstXI CCAn_nnnn'nTGG Cuts at: 0 92 165 819 1254 1300 1782 Size: 92 73 654 435 46 482 Fragments arranged by size: 654 482 435 92 73 46 BstYI r'GATC_y Cuts at: 0 34 305 476 1782 Size: 34 271 171 1306 Fragments arranged by size: 1306 271 171 34 BtgI C'CryG_G Cuts at: 0 961 1782 Size: 961 821 BtsI GCAGTG_nn' Cuts at: 0 320 551 649 825 1782 Size: 320 231 98 176 957 Fragments arranged by size: 957 320 231 176 98 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 454 489 1782 Size: 454 35 1293 Fragments arranged by size: 1293 454 35 DraIII CAC_nnn'GTG Cuts at: 0 267 782 1782 Size: 267 515 1000 Fragments arranged by size: 1000 515 267 EaeI y'GGCC_r Cuts at: 0 773 1402 1415 1472 1782 Size: 773 629 13 57 310 Fragments arranged by size: 773 629 310 57 13 EarI CTCTTCn'nnn_ Cuts at: 0 20 1782 Size: 20 1762 EcoICRI GAG'CTC Cuts at: 0 30 1512 1782 Size: 30 1482 270 Fragments arranged by size: 1482 270 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 585 1215 1276 1394 1592 1782 Size: 121 464 630 61 118 198 190 Fragments arranged by size: 630 464 198 190 121 118 61 EcoO109I rG'GnC_Cy Cuts at: 0 319 1782 Size: 319 1463 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1158 1782 Size: 1158 624 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 19 51 491 523 576 608 1226 1258 Size: 19 32 440 32 53 32 618 32 Cuts at: 1258 1414 1446 1782 Size: 156 32 336 Fragments arranged by size: 618 440 336 156 53 32 32 32 32 32 19 MscI TGG'CCA Cuts at: 0 775 1404 1782 Size: 775 629 378 Fragments arranged by size: 775 629 378 MslI CAynn'nnrTG Cuts at: 0 568 1258 1782 Size: 568 690 524 Fragments arranged by size: 690 568 524 MspA1I CmG'CkG Cuts at: 0 302 345 891 963 1071 1507 1782 Size: 302 43 546 72 108 436 275 Fragments arranged by size: 546 436 302 275 108 72 43 NspI r_CATG'y Cuts at: 0 392 464 1782 Size: 392 72 1318 Fragments arranged by size: 1318 392 72 PflMI CCAn_nnn'nTGG Cuts at: 0 782 1346 1782 Size: 782 564 436 Fragments arranged by size: 782 564 436 PfoI T'CCnGG_A Cuts at: 0 49 164 950 1103 1782 Size: 49 115 786 153 679 Fragments arranged by size: 786 679 153 115 49 PmlI CAC'GTG Cuts at: 0 1239 1782 Size: 1239 543 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1705 1737 1782 Size: 1705 32 45 Fragments arranged by size: 1705 45 32 PspOMI G'GGCC_C Cuts at: 0 318 803 1782 Size: 318 485 979 Fragments arranged by size: 979 485 318 PstI C_TGCA'G Cuts at: 0 1070 1782 Size: 1070 712 PvuII CAG'CTG Cuts at: 0 302 345 891 1071 1782 Size: 302 43 546 180 711 Fragments arranged by size: 711 546 302 180 43 SacI G_AGCT'C Cuts at: 0 32 1514 1782 Size: 32 1482 268 Fragments arranged by size: 1482 268 32 SacII CC_GC'GG Cuts at: 0 964 1782 Size: 964 818 SapI GCTCTTCn'nnn_ Cuts at: 0 20 1782 Size: 20 1762 SfcI C'TryA_G Cuts at: 0 1066 1782 Size: 1066 716 SmlI C'TyrA_G Cuts at: 0 1396 1782 Size: 1396 386 StyI C'CwwG_G Cuts at: 0 412 1142 1782 Size: 412 730 640 Fragments arranged by size: 730 640 412 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 384 461 781 895 1782 Size: 384 77 320 114 887 Fragments arranged by size: 887 384 320 114 77 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 306 370 1440 1782 Size: 306 64 1070 342 Fragments arranged by size: 1070 342 306 64 Enzymes that do cut: AarI AcuI AflIII AhdI AlwNI ApaI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BstEII BstXI BstYI BtgI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MscI MslI MspA1I NspI PflMI PfoI PmlI PpiI PspOMI PstI PvuII SacI SacII SapI SfcI SmlI StyI TaqII XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BfrBI BglII BmgBI BmrI BmtI BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI