Restriction map for
pFN21AE6794
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII CfoI Cfr10I
CpoI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AclI 848 AACGTT AA^CGTT
AhdI 3341 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2821 GATCC GGATC(4/5)
ApaI 2868 GGGCCC GGGCC^C
AspEI 3341 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1290 TGGCCA TGG^CCA
BseRI 1485 CTCCTC GAGGAG(10/8)
BsmI 1276 GAATGC GAATGC(1/-1)
BspHI 1734 TCATGA T^CATGA
BstYI 2820 AGATCC [GA]^GATC[CT]
DrdI 358 GACAATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1290 TGGCCA [CT]^GGCC[GA]
Eam1105I 3341 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 237 CCTTGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1267 GAATTC G^AATTC
HapII 1638 CCGG C^CGG
HpaII 1638 CCGG C^CGG
MflI 2820 AGATCC [GA]^GATC[CT]
MluNI 1290 TGGCCA TGG^CCA
MscI 1290 TGGCCA TGG^CCA
MslI 798 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1466 CAGCTG C[AC]G^C[GT]G
MspI 1638 CCGG C^CGG
PpuMI 726 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 848 AACGTT AA^CGTT
PvuII 1466 CAGCTG CAG^CTG
RcaI 1734 TCATGA T^CATGA
SacI 2095 GAGCTC GAGCT^C
SapI 1384 GAAGAGC GCTCTTC(1/4)
SphI 1998 GCATGC GCATG^C
SspI 3228 AATATT AAT^ATT
StuI 2007 AGGCCT AGG^CCT
XhoII 2820 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 2073 CCGC CCGC(-3/-1)
3335 CCGC
AlwNI 26 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2707 CAGCCACTG
Asp700I 466 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1606 GAACTTCTTC
AvaII 9 GGTCC G^G[AT]CC
727 GGACC
BanI 2379 GGCACC G^G[CT][GA]CC
2458 GGTGCC
BciVI 3048 GGATAC GTATCC(6/5)
3199 GTATCC
BsaAI 578 TACGTG [CT]AC^GT[GA]
1397 TACGTG
BsaBI 1372 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1780 GATGGGTATC
BsaI 1006 GAGACC GGTCTC(1/5)
1981 GAGACC
BspDI 613 ATCGAT AT^CGAT
2147 ATCGAT
Bst1107I 156 GTATAC GTA^TAC
2974 GTATAC
BstXI 716 CCAGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3203 CCACATGGTTGG
BstZ17I 156 GTATAC GTA^TAC
2974 GTATAC
ClaI 613 ATCGAT AT^CGAT
2147 ATCGAT
DraII 726 GGGACCT [GA]G^G[ACGT]CC[CT]
2868 GGGCCCC
DraIII 1793 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3076 CACTTTGTG
EcoO109I 726 GGGACCT [GA]G^G[ACGT]CC[CT]
2868 GGGCCCC
HpaI 1180 GTTAAC GTT^AAC
1746 GTTAAC
MamI 1372 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1780 GATGGGTATC
MfeI 555 CAATTG C^AATTG
632 CAATTG
MunI 555 CAATTG C^AATTG
632 CAATTG
NspI 1998 GCATGC [GA]CATG^[CT]
2059 ACATGC
ScaI 2206 AGTACT AGT^ACT
2633 AGTACT
XcmI 2682 CCAGTTCTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2855 CCATGCTACAGTTGG
XmnI 466 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1606 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
AseI AsnI AspHI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I