(Circular) (Six-base) MAPSORT of: pFN21AE6794.seq Check: 7789 from: 1 to: 8101 pFN21AE6794 8101 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5911 278 Size: 53 83 186 936 4375 2468 Fragments arranged by size: 4375 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2162 2259 4786 4980 5444 1937 Size: 225 97 2527 194 464 4594 Fragments arranged by size: 4594 2527 464 225 194 97 Acc65I G'GTAC_C Cuts at: 5422 5422 Size: 8101 AclI AA'CG_TT Cuts at: 1563 2854 6228 6601 1563 Size: 1291 3374 373 3063 Fragments arranged by size: 3374 3063 1291 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5060 5334 6164 7548 7816 1632 Size: 3428 274 830 1384 268 1917 Fragments arranged by size: 3428 1917 1384 830 274 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7904 AflIII A'CryG_T Cuts at: 1203 7000 7977 1203 Size: 5797 977 1327 Fragments arranged by size: 5797 1327 977 AgeI A'CCGG_T Cuts at: 6932 6932 Size: 8101 AhdI GACnn_n'nnGTC Cuts at: 5351 6830 5351 Size: 1479 6622 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8101 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4205 4387 4877 1184 Size: 3021 182 490 4408 Fragments arranged by size: 4408 3021 490 182 AlwNI CAG_nnn'CTG Cuts at: 2036 4717 5844 7416 2036 Size: 2681 1127 1572 2721 Fragments arranged by size: 2721 2681 1572 1127 ApaI G_GGCC'C Cuts at: 4877 4877 Size: 8101 ApaLI G'TGCA_C Cuts at: 6158 7314 6158 Size: 1156 6945 ApoI r'AATT_y Cuts at: 1493 2173 2218 2672 3113 3272 3934 4294 4423 Size: 680 45 454 441 159 662 360 129 Cuts at: 4423 4845 5410 5636 5788 7897 1493 Size: 422 565 226 152 2109 1697 Fragments arranged by size: 2109 1697 680 662 565 454 441 422 360 226 159 152 129 45 AseI AT'TA_AT Cuts at: 160 1032 2877 3168 4259 6655 160 Size: 872 1845 291 1091 2396 1606 Fragments arranged by size: 2396 1845 1606 1091 872 291 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 AvaI C'yCGr_G Cuts at: 1870 1943 5426 1870 Size: 73 3483 4545 Fragments arranged by size: 4545 3483 73 BamHI G'GATC_C Cuts at: 5431 5798 5431 Size: 367 7734 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4384 4463 5422 6877 618 Size: 325 217 627 2597 79 959 1455 1842 Fragments arranged by size: 2597 1842 1455 959 627 325 217 79 BanII G_rGCy'C Cuts at: 1391 3125 3181 4104 4877 5420 1391 Size: 1734 56 923 773 543 4072 Fragments arranged by size: 4072 1734 923 773 543 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3674 3966 5190 928 Size: 971 1775 292 1224 3839 Fragments arranged by size: 3839 1775 1224 971 292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6292 6326 1903 Size: 34 50 34 4271 34 3678 Fragments arranged by size: 4271 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5047 5215 5993 7203 5047 Size: 168 778 1210 5945 Fragments arranged by size: 5945 1210 778 168 BclI T'GATC_A Cuts at: 1548 1548 Size: 8101 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6712 136 Size: 107 122 71 631 605 5040 1525 Fragments arranged by size: 5040 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8096 8096 Size: 8101 BlpI GC'TnA_GC Cuts at: 5518 5518 Size: 8101 Bme1580I G_kGCm'C Cuts at: 1790 4877 6162 7318 1790 Size: 3087 1285 1156 2573 Fragments arranged by size: 3087 2573 1285 1156 BmgBI CAC'GTC Cuts at: 1318 5070 1318 Size: 3752 4349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2270 4044 5832 Size: 416 53 83 399 24 853 1774 1788 Cuts at: 5832 6790 442 Size: 958 1753 Fragments arranged by size: 1788 1774 1753 958 853 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2013 2067 3810 6761 1239 Size: 774 54 1743 2951 2579 Fragments arranged by size: 2951 2579 1743 774 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2729 3303 3657 5141 6226 7091 7389 2729 Size: 574 354 1484 1085 865 298 3441 Fragments arranged by size: 3441 1484 1085 865 574 354 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3005 3980 6764 882 Size: 617 1506 975 2784 2219 Fragments arranged by size: 2784 2219 1506 975 617 BsaAI yAC'GTr Cuts at: 493 1204 2585 3404 5892 493 Size: 711 1381 819 2488 2702 Fragments arranged by size: 2702 2488 1381 819 711 BsaBI GATnn'nnATC Cuts at: 3381 3789 8095 3381 Size: 408 4306 3387 Fragments arranged by size: 4306 3387 408 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5908 6290 6925 275 Size: 53 83 186 936 4375 382 635 1451 Fragments arranged by size: 4375 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6534 6932 7206 7353 7764 6534 Size: 398 274 147 411 6871 Fragments arranged by size: 6871 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2781 2811 3168 3198 1178 Size: 30 165 30 1378 30 357 30 6081 Fragments arranged by size: 6081 1378 357 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3481 1186 Size: 2295 5806 BseYI C'CCAG_C Cuts at: 7304 7800 7304 Size: 496 7605 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5551 6312 7340 1540 Size: 275 186 3550 761 1028 2301 Fragments arranged by size: 3550 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4032 4104 4371 6162 6247 7318 4032 Size: 72 267 1791 85 1071 4815 Fragments arranged by size: 4815 1791 1071 267 85 72 BsmI GAATG_Cn' Cuts at: 3287 5612 5705 3287 Size: 2325 93 5683 Fragments arranged by size: 5683 2325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3125 3181 4032 4104 4371 4877 5420 Size: 399 1335 56 851 72 267 506 543 Cuts at: 5420 6162 6247 7318 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1335 1071 851 742 543 506 399 267 85 72 56 BspEI T'CCGG_A Cuts at: 7764 7764 Size: 8101 BspHI T'CATG_A Cuts at: 3739 5990 7720 3739 Size: 2251 1730 4120 Fragments arranged by size: 4120 2251 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5456 844 Size: 4612 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5988 1394 Size: 429 4165 3507 Fragments arranged by size: 4165 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2118 2592 3786 4861 6596 6770 65 Size: 1183 870 474 1194 1075 1735 174 1396 Fragments arranged by size: 1735 1396 1194 1183 1075 870 474 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6745 6932 1700 Size: 110 4935 187 2869 Fragments arranged by size: 4935 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2245 2603 4633 4743 96 Size: 2149 358 2030 110 3454 Fragments arranged by size: 3454 2149 2030 358 110 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8101 BssSI C'ACGA_G Cuts at: 6161 7173 6161 Size: 1012 7089 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8101 BstBI TT'CG_AA Cuts at: 6905 6905 Size: 8101 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8101 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2728 5215 2011 Size: 717 2487 4897 Fragments arranged by size: 4897 2487 717 BstYI r'GATC_y Cuts at: 1972 4825 5431 5798 6183 6200 7652 7738 7750 Size: 2853 606 367 385 17 1452 86 12 Cuts at: 7750 8096 1972 Size: 346 1977 Fragments arranged by size: 2853 1977 1452 606 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2163 4981 2163 Size: 2818 5283 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7549 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7362 Fragments arranged by size: 7362 683 56 BtsI GCAGTG_nn' Cuts at: 3948 4371 5617 6411 6431 3948 Size: 423 1246 794 20 5618 Fragments arranged by size: 5618 1246 794 423 20 ClaI AT'CG_AT Cuts at: 1554 2619 4153 1554 Size: 1065 1534 5502 Fragments arranged by size: 5502 1534 1065 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3937 3972 4662 4697 529 Size: 35 3373 35 690 35 3933 Fragments arranged by size: 3933 3373 690 35 35 35 DraI TTT'AAA Cuts at: 3027 4225 4428 5405 3027 Size: 1198 203 977 5723 Fragments arranged by size: 5723 1198 977 203 DraIII CAC_nnn'GTG Cuts at: 3803 5086 3803 Size: 1283 6818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2369 7108 809 Size: 507 1053 4739 1802 Fragments arranged by size: 4739 1802 1053 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3295 5548 6437 Size: 54 1425 50 275 18 1465 2253 889 Cuts at: 6437 8066 8 Size: 1629 43 Fragments arranged by size: 2253 1629 1465 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5548 1537 Size: 275 3736 4090 Fragments arranged by size: 4090 3736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 3384 3499 5367 6031 1353 Size: 693 1338 115 1868 664 3423 Fragments arranged by size: 3423 1868 1338 693 664 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5329 6688 7062 7208 387 Size: 1064 138 3740 1359 374 146 1280 Fragments arranged by size: 3740 1359 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4102 4102 Size: 8101 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2067 3810 5060 5334 6164 6761 Size: 393 381 54 1743 1250 274 830 597 Cuts at: 6761 7548 7816 1239 Size: 787 268 1524 Fragments arranged by size: 1743 1524 1250 830 787 597 393 381 274 268 54 EcoNI CCTnn'n_nnAGG Cuts at: 2246 2246 Size: 8101 EcoO109I rG'GnC_Cy Cuts at: 2732 4874 2732 Size: 2142 5959 EcoRI G'AATT_C Cuts at: 3272 5410 5788 3272 Size: 2138 378 5585 Fragments arranged by size: 5585 2138 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5065 5065 Size: 8101 FspI TGC'GCA Cuts at: 6607 6607 Size: 8101 HaeII r_GCGC'y Cuts at: 1678 7248 1678 Size: 5570 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 3032 3064 3320 Size: 32 135 32 968 32 460 32 256 Cuts at: 3320 3352 4162 4194 4228 4260 4810 4842 5321 Size: 32 810 32 34 32 550 32 479 Cuts at: 5321 5337 5353 5369 6742 6774 6816 6848 1373 Size: 16 16 16 1373 32 42 32 2626 Fragments arranged by size: 2626 1373 968 810 550 479 460 256 135 42 34 32 32 32 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 2374 3187 3753 5445 5691 669 Size: 988 281 436 813 566 1692 246 3079 Fragments arranged by size: 3079 1692 988 813 566 436 281 246 HindIII A'AGCT_T Cuts at: 748 2112 4219 5042 748 Size: 1364 2107 823 3807 Fragments arranged by size: 3807 2107 1364 823 HpaI GTT'AAC Cuts at: 3187 3753 5691 3187 Size: 566 1938 5597 Fragments arranged by size: 5597 1938 566 KpnI G_GTAC'C Cuts at: 5426 5426 Size: 8101 MfeI C'AATT_G Cuts at: 2560 2637 5700 2560 Size: 77 3063 4961 Fragments arranged by size: 4961 3063 77 MluI A'CGCG_T Cuts at: 7977 7977 Size: 8101 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4358 7215 7399 1305 Size: 3053 2857 184 2007 Fragments arranged by size: 3053 2857 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3297 10 Size: 54 1425 343 1465 4814 Fragments arranged by size: 4814 1465 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2807 6059 6418 6577 518 Size: 648 843 798 3252 359 159 2042 Fragments arranged by size: 3252 2042 843 798 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3473 5517 6194 7342 7587 1606 Size: 1867 2044 677 1148 245 2120 Fragments arranged by size: 2120 2044 1867 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7549 NdeI CA'TA_TG Cuts at: 387 387 Size: 8101 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8101 NotI GC'GGCC_GC Cuts at: 5548 5548 Size: 8101 NspI r_CATG'y Cuts at: 1132 1229 4007 4068 5459 7004 1132 Size: 97 2778 61 1391 1545 2229 Fragments arranged by size: 2778 2229 1545 1391 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8101 PciI A'CATG_T Cuts at: 7000 7000 Size: 8101 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8101 PmeI GTTT'AAAC Cuts at: 5405 5405 Size: 8101 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6170 6202 7683 7715 6170 Size: 32 1481 32 6556 Fragments arranged by size: 6556 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2732 2732 Size: 8101 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8101 PsiI TTA'TAA Cuts at: 2309 5671 2309 Size: 3362 4739 PspOMI G'GGCC_C Cuts at: 4873 4873 Size: 8101 PstI C_TGCA'G Cuts at: 830 2926 3955 3985 4366 5453 830 Size: 2096 1029 30 381 1087 3478 Fragments arranged by size: 3478 2096 1087 1029 381 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8101 PvuII CAG'CTG Cuts at: 3473 3473 Size: 8101 SacI G_AGCT'C Cuts at: 4104 4104 Size: 8101 SalI G'TCGA_C Cuts at: 1936 5443 1936 Size: 3507 4594 SapI GCTCTTCn'nnn_ Cuts at: 3384 3384 Size: 8101 SbfI CC_TGCA'GG Cuts at: 5453 5453 Size: 8101 ScaI AGT'ACT Cuts at: 4213 4640 6349 8061 4213 Size: 427 1709 1712 4253 Fragments arranged by size: 4253 1712 1709 427 SfcI C'TryA_G Cuts at: 826 1045 2535 2695 2922 3426 3462 3951 3981 Size: 219 1490 160 227 504 36 489 30 Cuts at: 3981 4362 4780 4865 5314 5449 6584 7265 7456 Size: 381 418 85 449 135 1135 681 191 Cuts at: 7456 826 Size: 1471 Fragments arranged by size: 1490 1471 1135 681 504 489 449 418 381 227 219 191 160 135 85 36 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8101 SmaI CCC'GGG Cuts at: 1872 5428 1872 Size: 3556 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2708 3318 3672 5156 6205 7106 Size: 197 926 765 610 354 1484 1049 901 Cuts at: 7106 7368 820 Size: 262 1553 Fragments arranged by size: 1553 1484 1049 926 901 765 610 354 262 197 SnaBI TAC'GTA Cuts at: 493 5892 493 Size: 5399 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8101 SphI G_CATG'C Cuts at: 1132 4007 5459 1132 Size: 2875 1452 3774 Fragments arranged by size: 3774 2875 1452 SspI AAT'ATT Cuts at: 5 52 5235 6025 5 Size: 47 5183 790 2081 Fragments arranged by size: 5183 2081 790 47 StuI AGG'CCT Cuts at: 1517 4014 1517 Size: 2497 5604 StyI C'CwwG_G Cuts at: 513 1065 2836 2978 5049 5540 513 Size: 552 1771 142 2071 491 3074 Fragments arranged by size: 3074 2071 1771 552 491 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4015 6128 6145 6298 6483 6928 325 Size: 1255 2435 2113 17 153 185 445 1498 Fragments arranged by size: 2435 2113 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2245 2458 2603 Size: 274 80 33 51 1443 268 213 145 Cuts at: 2603 4211 4358 4633 4638 4743 6347 8059 96 Size: 1608 147 275 5 105 1604 1712 138 Fragments arranged by size: 1712 1608 1604 1443 275 274 268 213 147 145 138 105 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3787 3819 8061 8093 3787 Size: 32 4242 32 3795 Fragments arranged by size: 4242 3795 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8101 XbaI T'CTAG_A Cuts at: 5437 5437 Size: 8101 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4694 4867 4694 Size: 173 7928 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8101 XmaI C'CCGG_G Cuts at: 1870 5426 1870 Size: 3556 4545 XmnI GAAnn'nnTTC Cuts at: 2475 3615 6230 2475 Size: 1140 2615 4346 Fragments arranged by size: 4346 2615 1140 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5909 276 Size: 53 83 186 936 4375 2468 Fragments arranged by size: 4375 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI