(Circular) (Six-base) MAPSORT of: pFN21AE6792.seq Check: 4200 from: 1 to: 7075 pFN21AE6792 7075 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4885 278 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4418 1937 Size: 2481 4594 Acc65I G'GTAC_C Cuts at: 4396 4396 Size: 7075 AclI AA'CG_TT Cuts at: 1563 5202 5575 1563 Size: 3639 373 3063 Fragments arranged by size: 3639 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 2510 2835 4284 5138 6522 6790 1632 Size: 495 383 325 1449 854 1384 268 1917 Fragments arranged by size: 1917 1449 1384 854 495 383 325 268 AflII C'TTAA_G Cuts at: 820 1017 2072 2524 3200 820 Size: 197 1055 452 676 4695 Fragments arranged by size: 4695 1055 676 452 197 AflIII A'CryG_T Cuts at: 1203 2473 2791 3164 3369 5974 6951 1203 Size: 1270 318 373 205 2605 977 1327 Fragments arranged by size: 2605 1327 1270 977 373 318 205 AgeI A'CCGG_T Cuts at: 5906 5906 Size: 7075 AhdI GACnn_n'nnGTC Cuts at: 5804 5804 Size: 7075 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7075 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3327 3427 1184 Size: 2143 100 4832 Fragments arranged by size: 4832 2143 100 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3412 3444 3412 Size: 32 7043 AlwNI CAG_nnn'CTG Cuts at: 4818 6390 4818 Size: 1572 5503 ApaLI G'TGCA_C Cuts at: 5132 6288 5132 Size: 1156 5919 ApoI r'AATT_y Cuts at: 1493 3421 3624 3631 3655 4384 4610 4762 6871 Size: 1928 203 7 24 729 226 152 2109 Cuts at: 6871 1493 Size: 1697 Fragments arranged by size: 2109 1928 1697 729 226 203 152 24 7 AseI AT'TA_AT Cuts at: 160 1032 4100 5629 160 Size: 872 3068 1529 1606 Fragments arranged by size: 3068 1606 1529 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 AvaI C'yCGr_G Cuts at: 1870 1943 3924 4400 1870 Size: 73 1981 476 4545 Fragments arranged by size: 4545 1981 476 73 AvrII C'CTAG_G Cuts at: 3811 3811 Size: 7075 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3358 3391 3358 Size: 33 7042 BamHI G'GATC_C Cuts at: 2594 4405 4772 2594 Size: 1811 367 4897 Fragments arranged by size: 4897 1811 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3311 3830 3973 4396 5851 Size: 325 217 627 1524 519 143 423 1455 Cuts at: 5851 618 Size: 1842 Fragments arranged by size: 1842 1524 1455 627 519 423 325 217 143 BanII G_rGCy'C Cuts at: 1391 2348 2448 3098 4394 1391 Size: 957 100 650 1296 4072 Fragments arranged by size: 4072 1296 957 650 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5266 5300 1903 Size: 34 3329 34 3678 Fragments arranged by size: 3678 3329 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2739 3359 4967 6177 2739 Size: 620 1608 1210 3637 Fragments arranged by size: 3637 1608 1210 620 BclI T'GATC_A Cuts at: 1548 2638 3595 1548 Size: 1090 957 5028 Fragments arranged by size: 5028 1090 957 BfrBI ATG'CAT Cuts at: 2391 2391 Size: 7075 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3554 5686 136 Size: 107 122 71 631 605 1882 2132 1525 Fragments arranged by size: 2132 1882 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2019 7070 2019 Size: 5051 2024 BlpI GC'TnA_GC Cuts at: 2150 4492 2150 Size: 2342 4733 Bme1580I G_kGCm'C Cuts at: 1790 2884 3770 3976 5136 6292 1790 Size: 1094 886 206 1160 1156 2573 Fragments arranged by size: 2573 1160 1156 1094 886 206 BmgBI CAC'GTC Cuts at: 1318 2224 1318 Size: 906 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2457 2715 3117 Size: 416 53 83 399 24 1040 258 402 Cuts at: 3117 4806 5764 442 Size: 1689 958 1753 Fragments arranged by size: 1753 1689 1040 958 416 402 399 258 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7075 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 7075 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 4011 5735 1239 Size: 1085 1687 1724 2579 Fragments arranged by size: 2579 1724 1687 1085 Bpu10I CC'TnA_GC Cuts at: 2579 3097 2579 Size: 518 6557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2435 2661 5200 6065 6363 2435 Size: 226 2539 865 298 3147 Fragments arranged by size: 3147 2539 865 298 226 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2405 5738 882 Size: 617 906 3333 2219 Fragments arranged by size: 3333 2219 906 617 BsaAI yAC'GTr Cuts at: 493 1204 3370 4866 493 Size: 711 2166 1496 2702 Fragments arranged by size: 2702 2166 1496 711 BsaBI GATnn'nnATC Cuts at: 7069 7069 Size: 7075 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4882 5264 5899 275 Size: 53 83 186 936 3349 382 635 1451 Fragments arranged by size: 3349 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5508 5906 6180 6327 6738 5508 Size: 398 274 147 411 5845 Fragments arranged by size: 5845 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2330 2360 1178 Size: 30 165 30 927 30 5893 Fragments arranged by size: 5893 927 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3275 1186 Size: 2089 4986 BseYI C'CCAG_C Cuts at: 3022 3762 6278 6774 3022 Size: 740 2516 496 3323 Fragments arranged by size: 3323 2516 740 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 2189 3523 1163 Size: 573 300 153 1334 4715 Fragments arranged by size: 4715 1334 573 300 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4525 5286 6314 1540 Size: 275 186 2524 761 1028 2301 Fragments arranged by size: 2524 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5136 5221 6292 5136 Size: 85 1071 5919 Fragments arranged by size: 5919 1071 85 BsmI GAATG_Cn' Cuts at: 4586 4679 4586 Size: 93 6982 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2348 2448 2884 3098 3770 3976 4394 Size: 399 558 100 436 214 672 206 418 Cuts at: 4394 5136 5221 6292 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 672 558 436 418 399 214 206 100 85 BspEI T'CCGG_A Cuts at: 6738 6738 Size: 7075 BspHI T'CATG_A Cuts at: 3016 3340 4964 6694 3016 Size: 324 1624 1730 3397 Fragments arranged by size: 3397 1730 1624 324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4430 844 Size: 3586 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4962 1394 Size: 429 3139 3507 Fragments arranged by size: 3507 3139 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5570 5744 65 Size: 1183 4322 174 1396 Fragments arranged by size: 4322 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5719 5906 1700 Size: 110 3909 187 2869 Fragments arranged by size: 3909 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4222 96 Size: 4126 2949 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7075 BssSI C'ACGA_G Cuts at: 5135 6147 5135 Size: 1012 6063 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7075 BstBI TT'CG_AA Cuts at: 3628 5879 3628 Size: 2251 4824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7075 BstYI r'GATC_y Cuts at: 1972 2019 2594 3506 4405 4772 5157 5174 6626 Size: 47 575 912 899 367 385 17 1452 Cuts at: 6626 6712 6724 7070 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 912 899 575 385 367 346 86 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 3549 3795 513 Size: 552 2484 246 3793 Fragments arranged by size: 3793 2484 552 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6336 Fragments arranged by size: 6336 683 56 BtsI GCAGTG_nn' Cuts at: 3527 3955 4591 5385 5405 3527 Size: 428 636 794 20 5197 Fragments arranged by size: 5197 794 636 428 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7075 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7040 DraI TTT'AAA Cuts at: 3142 3637 3862 4379 3142 Size: 495 225 517 5838 Fragments arranged by size: 5838 517 495 225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3336 6082 809 Size: 507 2020 2746 1802 Fragments arranged by size: 2746 2020 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4522 5411 7040 Size: 54 1425 50 275 18 2692 889 1629 Cuts at: 7040 8 Size: 43 Fragments arranged by size: 2692 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4522 1537 Size: 275 2710 4090 Fragments arranged by size: 4090 2710 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3157 5005 1353 Size: 1804 1848 3423 Fragments arranged by size: 3423 1848 1804 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5662 6036 6182 387 Size: 1064 138 4073 374 146 1280 Fragments arranged by size: 4073 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2324 2510 2835 4011 4284 5138 Size: 393 495 197 186 325 1176 273 854 Cuts at: 5138 5735 6522 6790 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1176 854 787 597 495 393 325 273 268 197 186 EcoNI CCTnn'n_nnAGG Cuts at: 3810 3810 Size: 7075 EcoRI G'AATT_C Cuts at: 3421 3624 4384 4762 3421 Size: 203 760 378 5734 Fragments arranged by size: 5734 760 378 203 EcoRV GAT'ATC Cuts at: 2801 2801 Size: 7075 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2170 2170 Size: 7075 FspI TGC'GCA Cuts at: 5581 5581 Size: 7075 HaeII r_GCGC'y Cuts at: 1678 6222 1678 Size: 4544 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3818 3850 5716 5748 5790 Size: 32 135 32 2246 32 1866 32 42 Cuts at: 5790 5822 1373 Size: 32 2626 Fragments arranged by size: 2626 2246 1866 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3583 4419 4665 669 Size: 988 281 1645 836 246 3079 Fragments arranged by size: 3079 1645 988 836 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7075 HpaI GTT'AAC Cuts at: 3583 4665 3583 Size: 1082 5993 KpnI G_GTAC'C Cuts at: 4400 4400 Size: 7075 MfeI C'AATT_G Cuts at: 3118 4674 3118 Size: 1556 5519 MluI A'CGCG_T Cuts at: 2791 6951 2791 Size: 4160 2915 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2084 2776 6189 6373 1305 Size: 779 692 3413 184 2007 Fragments arranged by size: 3413 2007 779 692 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5253 Fragments arranged by size: 5253 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 4079 5033 5392 5551 518 Size: 648 1224 1689 954 359 159 2042 Fragments arranged by size: 2042 1689 1224 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4491 5168 6316 6561 1606 Size: 2885 677 1148 245 2120 Fragments arranged by size: 2885 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3549 3795 513 Size: 552 2484 246 3793 Fragments arranged by size: 3793 2484 552 246 NdeI CA'TA_TG Cuts at: 387 387 Size: 7075 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7075 NotI GC'GGCC_GC Cuts at: 4522 4522 Size: 7075 NsiI A_TGCA'T Cuts at: 2393 2393 Size: 7075 NspI r_CATG'y Cuts at: 1132 1229 2391 2477 3168 3964 4433 5978 1132 Size: 97 1162 86 691 796 469 1545 2229 Fragments arranged by size: 2229 1545 1162 796 691 469 97 86 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7075 PciI A'CATG_T Cuts at: 2473 3164 5974 2473 Size: 691 2810 3574 Fragments arranged by size: 3574 2810 691 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7075 PfoI T'CCnGG_A Cuts at: 2590 4027 2590 Size: 1437 5638 PmeI GTTT'AAAC Cuts at: 4379 4379 Size: 7075 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5144 5176 6657 6689 5144 Size: 32 1481 32 5530 Fragments arranged by size: 5530 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7075 PsiI TTA'TAA Cuts at: 3450 4645 3450 Size: 1195 5880 PstI C_TGCA'G Cuts at: 830 2205 3894 4427 830 Size: 1375 1689 533 3478 Fragments arranged by size: 3478 1689 1375 533 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7075 SalI G'TCGA_C Cuts at: 1936 4417 1936 Size: 2481 4594 SbfI CC_TGCA'GG Cuts at: 4427 4427 Size: 7075 ScaI AGT'ACT Cuts at: 2615 2971 3410 5323 7035 2615 Size: 356 439 1913 1712 2655 Fragments arranged by size: 2655 1913 1712 439 356 SexAI A'CCwGG_T Cuts at: 3005 3307 3005 Size: 302 6773 SfcI C'TryA_G Cuts at: 826 1045 2201 3033 3319 3674 3890 4423 5558 Size: 219 1156 832 286 355 216 533 1135 Cuts at: 5558 6239 6430 826 Size: 681 191 1471 Fragments arranged by size: 1471 1156 1135 832 681 533 355 286 219 216 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7075 SmaI CCC'GGG Cuts at: 1872 3926 4402 1872 Size: 2054 476 4545 Fragments arranged by size: 4545 2054 476 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 2450 2524 2676 3200 5179 Size: 197 926 129 378 74 152 524 1979 Cuts at: 5179 6080 6342 820 Size: 901 262 1553 Fragments arranged by size: 1979 1553 926 901 524 378 262 197 152 129 74 SnaBI TAC'GTA Cuts at: 493 4866 493 Size: 4373 2702 SpeI A'CTAG_T Cuts at: 152 2053 152 Size: 1901 5174 SphI G_CATG'C Cuts at: 1132 4433 1132 Size: 3301 3774 SspI AAT'ATT Cuts at: 5 52 4999 5 Size: 47 4947 2081 Fragments arranged by size: 4947 2081 47 StuI AGG'CCT Cuts at: 1517 2456 1517 Size: 939 6136 StyI C'CwwG_G Cuts at: 513 1065 3549 3795 3811 4514 513 Size: 552 2484 246 16 703 3074 Fragments arranged by size: 3074 2484 703 552 246 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2622 3957 5102 5119 5272 5457 5902 Size: 1255 1042 1335 1145 17 153 185 445 Cuts at: 5902 325 Size: 1498 Fragments arranged by size: 1498 1335 1255 1145 1042 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2613 2969 3408 Size: 274 80 33 51 1443 636 356 439 Cuts at: 3408 4222 5321 7033 96 Size: 814 1099 1712 138 Fragments arranged by size: 1712 1443 1099 814 636 439 356 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7035 7067 7035 Size: 32 7043 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7075 XbaI T'CTAG_A Cuts at: 2947 4411 2947 Size: 1464 5611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3533 3793 3962 3533 Size: 260 169 6646 Fragments arranged by size: 6646 260 169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7075 XmaI C'CCGG_G Cuts at: 1870 3924 4400 1870 Size: 2054 476 4545 Fragments arranged by size: 4545 2054 476 XmnI GAAnn'nnTTC Cuts at: 5204 5204 Size: 7075 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4883 276 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI