################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI KspI MluI MroI MspI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1544 GTAGAC GT^[AC][GT]AC AclI 856 AACGTT AA^CGTT Alw44I 172 GTGCAC G^TGCAC ApaLI 172 GTGCAC G^TGCAC Asp700I 1647 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 172 GTGCAC G[AT]GC[AT]^C BanI 2124 GGCACC G^G[CT][GA]CC BanII 163 GAGCCC G[GA]GC[CT]^C BbsI 1135 GAAGAC GAAGAC(2/6) BclI 293 TGATCA T^GATCA BglII 2003 AGATCT A^GATCT BpuAI 1135 GAAGAC GAAGAC(2/6) BsaBI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 959 GAGACC GGTCTC(1/5) BseRI 74 GAGGAG GAGGAG(10/8) BsiHKAI 172 GTGCAC G[AT]GC[AT]^C BsmAI 959 GAGAC GTCTC(1/5) BspLU11I 2280 ACATGT A^CATGT BspMI 2065 ACCTGC ACCTGC(4/8) BsrDI 1583 GCAATG GCAATG(2/0) BssSI 1174 CACGAG CACGAG(-5/-1) BstXI 1173 CCACGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1209 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 483 CCATGG C^C[GA][CT]GG EcoNI 441 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1246 ATGCAT ATGCA^T FbaI 293 TGATCA T^GATCA HincII 672 GTTAAC GT[CT]^[GA]AC HindII 672 GTTAAC GT[CT]^[GA]AC HindIII 1069 AAGCTT A^AGCTT HpaI 672 GTTAAC GTT^AAC MamI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 709 CAATTG C^AATTG MspA1I 766 CAGCGG C[AC]G^C[GT]G MunI 709 CAATTG C^AATTG NcoI 483 CCATGG C^CATGG NdeI 1799 CATATG CA^TATG NsiI 1246 ATGCAT ATGCA^T PflMI 1916 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 508 GAGTC GAGTC(4/5) PpuMI 500 GGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 856 AACGTT AA^CGTT PstI 1284 CTGCAG CTGCA^G TaqI 1017 TCGA T^CGA TthHB8I 1017 TCGA T^CGA Van91I 1916 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 117 TCTAGA T^CTAGA XmnI 1647 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 1273 AAATTT [GA]^AATT[CT] 1519 AAATTT AflIII 1669 ACGTGT A^C[GA][CT]GT 2280 ACATGT ApoI 1273 AAATTT [GA]^AATT[CT] 1519 AAATTT BalI 228 TGGCCA TGG^CCA 1707 TGGCCA DraII 244 AGGCCCT [GA]G^G[ACGT]CC[CT] 500 GGGTCCC EaeI 228 TGGCCA [CT]^GGCC[GA] 1707 TGGCCA EcoO109I 244 AGGCCCT [GA]G^G[ACGT]CC[CT] 500 GGGTCCC EcoRV 806 GATATC GAT^ATC 1822 GATATC MaeII 857 ACGT A^CGT 1669 ACGT MluNI 228 TGGCCA TGG^CCA 1707 TGGCCA MscI 228 TGGCCA TGG^CCA 1707 TGGCCA ScaI 1309 AGTACT AGT^ACT 1373 AGTACT SphI 435 GCATGC GCATG^C 1248 GCATGC SspI 799 AATATT AAT^ATT 1298 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII