(Circular) (Six-base) MAPSORT of: pFN21AE6791.seq Check: 4776 from: 1 to: 7033 pFN21AE6791 7033 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4843 278 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3550 4376 1937 Size: 1613 826 4594 Fragments arranged by size: 4594 1613 826 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 2862 5160 5533 1563 Size: 1299 2298 373 3063 Fragments arranged by size: 3063 2298 1299 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2756 3395 5096 6480 6748 1632 Size: 1124 639 1701 1384 268 1917 Fragments arranged by size: 1917 1701 1384 1124 639 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6836 AflIII A'CryG_T Cuts at: 1203 3674 4285 5932 6909 1203 Size: 2471 611 1647 977 1327 Fragments arranged by size: 2471 1647 1327 977 611 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 4243 1166 Size: 3077 3956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3545 1184 Size: 2361 4672 AlwNI CAG_nnn'CTG Cuts at: 4776 6348 4776 Size: 1572 5461 ApaLI G'TGCA_C Cuts at: 2177 5090 6246 2177 Size: 2913 1156 2964 Fragments arranged by size: 2964 2913 1156 ApoI r'AATT_y Cuts at: 1493 3278 3524 4342 4568 4720 6829 1493 Size: 1785 246 818 226 152 2109 1697 Fragments arranged by size: 2109 1785 1697 818 246 226 152 AseI AT'TA_AT Cuts at: 160 1032 5587 160 Size: 872 4555 1606 Fragments arranged by size: 4555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 4358 1870 Size: 73 2415 4545 Fragments arranged by size: 4545 2415 73 BamHI G'GATC_C Cuts at: 4363 4730 4363 Size: 367 6666 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4129 4354 5809 618 Size: 325 217 627 2342 225 1455 1842 Fragments arranged by size: 2342 1842 1455 627 325 225 217 BanII G_rGCy'C Cuts at: 1391 2172 4352 1391 Size: 781 2180 4072 Fragments arranged by size: 4072 2180 781 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3147 928 Size: 971 1248 4814 Fragments arranged by size: 4814 1248 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4925 6135 4925 Size: 1210 5823 BclI T'GATC_A Cuts at: 1548 2298 1548 Size: 750 6283 BfrBI ATG'CAT Cuts at: 3253 3253 Size: 7033 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5644 136 Size: 107 122 71 631 605 3972 1525 Fragments arranged by size: 3972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4008 7028 4008 Size: 3020 4013 BlpI GC'TnA_GC Cuts at: 4450 4450 Size: 7033 Bme1580I G_kGCm'C Cuts at: 1790 2181 3935 4132 5094 6250 1790 Size: 391 1754 197 962 1156 2573 Fragments arranged by size: 2573 1754 1156 962 391 197 BmgBI CAC'GTC Cuts at: 1318 3675 1318 Size: 2357 4676 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2251 3211 4764 Size: 416 53 83 399 24 834 960 1553 Cuts at: 4764 5722 442 Size: 958 1753 Fragments arranged by size: 1753 1553 960 958 834 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3750 3750 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2256 2435 3144 5693 1239 Size: 1017 179 709 2549 2579 Fragments arranged by size: 2579 2549 1017 709 179 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5158 6023 6321 5158 Size: 865 298 5870 Fragments arranged by size: 5870 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2958 5696 882 Size: 617 1459 2738 2219 Fragments arranged by size: 2738 2219 1459 617 BsaAI yAC'GTr Cuts at: 493 1204 4824 493 Size: 711 3620 2702 Fragments arranged by size: 3620 2702 711 BsaBI GATnn'nnATC Cuts at: 4013 7027 4013 Size: 3014 4019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4840 5222 5857 275 Size: 53 83 186 936 3307 382 635 1451 Fragments arranged by size: 3307 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5466 5864 6138 6285 6696 5466 Size: 398 274 147 411 5803 Fragments arranged by size: 5803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3117 3147 3623 3653 1178 Size: 30 165 30 1714 30 476 30 4558 Fragments arranged by size: 4558 1714 476 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2094 1186 Size: 908 6125 BseYI C'CCAG_C Cuts at: 3927 6236 6732 3927 Size: 2309 496 4228 Fragments arranged by size: 4228 2309 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2391 2864 3647 3917 1163 Size: 573 655 473 783 270 4279 Fragments arranged by size: 4279 783 655 573 473 270 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4483 5244 6272 1540 Size: 275 186 2482 761 1028 2301 Fragments arranged by size: 2482 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 5094 5179 6250 2181 Size: 2913 85 1071 2964 Fragments arranged by size: 2964 2913 1071 85 BsmI GAATG_Cn' Cuts at: 2562 3658 3886 4544 4637 2562 Size: 1096 228 658 93 4958 Fragments arranged by size: 4958 1096 658 228 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2181 3935 4132 4352 5094 5179 Size: 399 382 9 1754 197 220 742 85 Cuts at: 5179 6250 1391 Size: 1071 2174 Fragments arranged by size: 2174 1754 1071 742 399 382 220 197 85 9 BspEI T'CCGG_A Cuts at: 6696 6696 Size: 7033 BspHI T'CATG_A Cuts at: 4922 6652 4922 Size: 1730 5303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4079 4388 844 Size: 3235 309 3489 Fragments arranged by size: 3489 3235 309 BsrBI CCG'CTC Cuts at: 1394 1823 4920 1394 Size: 429 3097 3507 Fragments arranged by size: 3507 3097 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3595 5528 5702 65 Size: 1183 2347 1933 174 1396 Fragments arranged by size: 2347 1933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5677 5864 1700 Size: 110 3867 187 2869 Fragments arranged by size: 3867 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 3179 5093 6105 3179 Size: 1914 1012 4107 Fragments arranged by size: 4107 1914 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2446 3519 3728 794 Size: 1652 1073 209 4099 Fragments arranged by size: 4099 1652 1073 209 BstBI TT'CG_AA Cuts at: 5837 5837 Size: 7033 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7033 BstXI CCAn_nnnn'nTGG Cuts at: 3185 3185 Size: 7033 BstYI r'GATC_y Cuts at: 1972 2029 2037 3448 3636 4008 4363 4730 5115 Size: 57 8 1411 188 372 355 367 385 Cuts at: 5115 5132 6584 6670 6682 7028 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1411 385 372 367 355 346 188 86 57 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2004 2488 513 Size: 552 939 484 5058 Fragments arranged by size: 5058 939 552 484 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2166 3443 4111 4549 5343 5363 2100 Size: 66 1277 668 438 794 20 3770 Fragments arranged by size: 3770 1277 794 668 438 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6998 DraI TTT'AAA Cuts at: 4337 4337 Size: 7033 DraIII CAC_nnn'GTG Cuts at: 3219 3219 Size: 7033 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 3712 4480 Size: 54 1425 50 275 18 403 1479 768 Cuts at: 4480 5369 6998 8 Size: 889 1629 43 Fragments arranged by size: 1629 1479 1425 889 768 403 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4480 1537 Size: 275 2668 4090 Fragments arranged by size: 4090 2668 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2119 2199 2679 4963 1353 Size: 766 80 480 2284 3423 Fragments arranged by size: 3423 2284 766 480 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2435 2756 3144 3395 5096 5693 Size: 393 624 179 321 388 251 1701 597 Cuts at: 5693 6480 6748 1239 Size: 787 268 1524 Fragments arranged by size: 1701 1524 787 624 597 393 388 321 268 251 179 EcoNI CCTnn'n_nnAGG Cuts at: 2450 2450 Size: 7033 EcoO109I rG'GnC_Cy Cuts at: 2250 2506 2250 Size: 256 6777 EcoRI G'AATT_C Cuts at: 4342 4720 4342 Size: 378 6655 EcoRV GAT'ATC Cuts at: 2813 3829 2813 Size: 1016 6017 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 6180 1678 Size: 4502 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2358 2390 3586 3618 3623 Size: 32 135 32 786 32 1196 32 5 Cuts at: 3623 3655 5674 5706 5748 5780 1373 Size: 32 2019 32 42 32 2626 Fragments arranged by size: 2626 2019 1196 786 135 42 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 2679 4377 4623 669 Size: 988 281 741 1698 246 3079 Fragments arranged by size: 3079 1698 988 741 281 246 HindIII A'AGCT_T Cuts at: 748 3074 748 Size: 2326 4707 HpaI GTT'AAC Cuts at: 2679 4623 2679 Size: 1944 5089 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 2714 4632 2714 Size: 1918 5115 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3797 6147 6331 1305 Size: 2492 2350 184 2007 Fragments arranged by size: 2492 2350 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2235 3714 10 Size: 54 1425 343 403 1479 3329 Fragments arranged by size: 3329 1479 1425 403 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2493 3912 4243 4991 5350 5509 518 Size: 648 1327 1419 331 748 359 159 2042 Fragments arranged by size: 2042 1419 1327 748 648 359 331 159 MspA1I CmG'CkG Cuts at: 1606 2773 4449 5126 6274 6519 1606 Size: 1167 1676 677 1148 245 2120 Fragments arranged by size: 2120 1676 1167 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2488 513 Size: 552 939 484 5058 Fragments arranged by size: 5058 939 552 484 NdeI CA'TA_TG Cuts at: 387 3805 387 Size: 3418 3615 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NsiI A_TGCA'T Cuts at: 3255 3255 Size: 7033 NspI r_CATG'y Cuts at: 1132 1229 2444 3257 4289 4391 5936 1132 Size: 97 1215 813 1032 102 1545 2229 Fragments arranged by size: 2229 1545 1215 1032 813 102 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7033 PciI A'CATG_T Cuts at: 4285 5932 4285 Size: 1647 5386 PflMI CCAn_nnn'nTGG Cuts at: 1108 3927 1108 Size: 2819 4214 PfoI T'CCnGG_A Cuts at: 2326 2451 2469 2326 Size: 125 18 6890 Fragments arranged by size: 6890 125 18 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5102 5134 6615 6647 5102 Size: 32 1481 32 5488 Fragments arranged by size: 5488 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2506 2506 Size: 7033 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 3009 4603 3009 Size: 1594 5439 PstI C_TGCA'G Cuts at: 830 3293 4385 830 Size: 2463 1092 3478 Fragments arranged by size: 3478 2463 1092 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SanDI GG'GwC_CC Cuts at: 2506 2506 Size: 7033 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 3316 3380 5281 6993 3316 Size: 64 1901 1712 3356 Fragments arranged by size: 3356 1901 1712 64 SfcI C'TryA_G Cuts at: 826 1045 2341 2561 3236 3259 3289 3891 4381 Size: 219 1296 220 675 23 30 602 490 Cuts at: 4381 5516 6197 6388 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1296 1135 681 675 602 490 220 219 191 30 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7033 SmaI CCC'GGG Cuts at: 1872 4360 1872 Size: 2488 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5137 6038 6300 820 Size: 197 926 3194 901 262 1553 Fragments arranged by size: 3194 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4824 493 Size: 4331 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 2444 3257 4391 1132 Size: 1312 813 1134 3774 Fragments arranged by size: 3774 1312 1134 813 SspI AAT'ATT Cuts at: 5 52 2806 3305 4957 5 Size: 47 2754 499 1652 2081 Fragments arranged by size: 2754 2081 1652 499 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 2004 2488 2517 3763 4472 513 Size: 552 939 484 29 1246 709 3074 Fragments arranged by size: 3074 1246 939 709 552 484 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 3230 3767 3776 5060 5077 5230 Size: 1255 573 1077 537 9 1284 17 153 Cuts at: 5230 5415 5860 325 Size: 185 445 1498 Fragments arranged by size: 1498 1284 1255 1077 573 537 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3314 3378 3470 Size: 274 80 33 51 1443 1337 64 92 Cuts at: 3470 5279 6991 96 Size: 1809 1712 138 Fragments arranged by size: 1809 1712 1443 1337 274 138 92 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4197 4229 6993 7025 4197 Size: 32 2764 32 4205 Fragments arranged by size: 4205 2764 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7033 XbaI T'CTAG_A Cuts at: 2122 4369 2122 Size: 2247 4786 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 4358 1870 Size: 2488 4545 XmnI GAAnn'nnTTC Cuts at: 3656 5162 3656 Size: 1506 5527 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4841 276 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI