(Linear) (Six-base) MAPSORT of: KIEE6791.seq Check: 2110 from: 1 to: 2328 KIEE6791.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. AccI GT'mk_AC Cuts at: 0 1545 2328 Size: 1545 783 AclI AA'CG_TT Cuts at: 0 857 2328 Size: 857 1471 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 751 1390 2328 Size: 751 639 938 Fragments arranged by size: 938 751 639 AflIII A'CryG_T Cuts at: 0 1669 2280 2328 Size: 1669 611 48 Fragments arranged by size: 1669 611 48 AleI CACnn'nnGTG Cuts at: 0 2238 2328 Size: 2238 90 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1540 2328 Size: 1540 788 ApaLI G'TGCA_C Cuts at: 0 172 2328 Size: 172 2156 ApoI r'AATT_y Cuts at: 0 1273 1519 2328 Size: 1273 246 809 Fragments arranged by size: 1273 809 246 BanI G'GyrC_C Cuts at: 0 2124 2328 Size: 2124 204 BanII G_rGCy'C Cuts at: 0 167 2328 Size: 167 2161 BbsI GAAGACnn'nnnn_ Cuts at: 0 1142 2328 Size: 1142 1186 BclI T'GATC_A Cuts at: 0 293 2328 Size: 293 2035 BfrBI ATG'CAT Cuts at: 0 1248 2328 Size: 1248 1080 BglII A'GATC_T Cuts at: 0 2003 2328 Size: 2003 325 Bme1580I G_kGCm'C Cuts at: 0 176 1930 2127 2328 Size: 176 1754 197 201 Fragments arranged by size: 1754 201 197 176 BmgBI CAC'GTC Cuts at: 0 1670 2328 Size: 1670 658 BmrI ACTGGGnnnn_n' Cuts at: 0 246 1206 2328 Size: 246 960 1122 Fragments arranged by size: 1122 960 246 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1745 2328 Size: 1745 583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 430 1139 2328 Size: 251 179 709 1189 Fragments arranged by size: 1189 709 251 179 BsaI GGTCTCn'nnnn_ Cuts at: 0 953 2328 Size: 953 1375 BsaBI GATnn'nnATC Cuts at: 0 2008 2328 Size: 2008 320 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1112 1142 1618 1648 2328 Size: 1112 30 476 30 680 Fragments arranged by size: 1112 680 476 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 89 2328 Size: 89 2239 BseYI C'CCAG_C Cuts at: 0 1922 2328 Size: 1922 406 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 386 859 1642 1912 2328 Size: 386 473 783 270 416 Fragments arranged by size: 783 473 416 386 270 BsiHKAI G_wGCw'C Cuts at: 0 176 2328 Size: 176 2152 BsmI GAATG_Cn' Cuts at: 0 557 1653 1881 2328 Size: 557 1096 228 447 Fragments arranged by size: 1096 557 447 228 Bsp1286I G_dGCh'C Cuts at: 0 167 176 1930 2127 2328 Size: 167 9 1754 197 201 Fragments arranged by size: 1754 201 197 167 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2074 2328 Size: 2074 254 BsrDI GCAATG_nn' Cuts at: 0 1590 2328 Size: 1590 738 BssSI C'ACGA_G Cuts at: 0 1174 2328 Size: 1174 1154 BstAPI GCAn_nnn'nTGC Cuts at: 0 441 1514 1723 2328 Size: 441 1073 209 605 Fragments arranged by size: 1073 605 441 209 BstXI CCAn_nnnn'nTGG Cuts at: 0 1180 2328 Size: 1180 1148 BstYI r'GATC_y Cuts at: 0 24 32 1443 1631 2003 2328 Size: 24 8 1411 188 372 325 Fragments arranged by size: 1411 372 325 188 24 8 BtgI C'CryG_G Cuts at: 0 483 2328 Size: 483 1845 BtsI GCAGTG_nn' Cuts at: 0 95 161 1438 2106 2328 Size: 95 66 1277 668 222 Fragments arranged by size: 1277 668 222 95 66 DraIII CAC_nnn'GTG Cuts at: 0 1214 2328 Size: 1214 1114 EaeI y'GGCC_r Cuts at: 0 228 1707 2328 Size: 228 1479 621 Fragments arranged by size: 1479 621 228 EarI CTCTTCn'nnn_ Cuts at: 0 114 194 674 2328 Size: 114 80 480 1654 Fragments arranged by size: 1654 480 114 80 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 430 751 1139 1390 2328 Size: 251 179 321 388 251 938 Fragments arranged by size: 938 388 321 251 251 179 EcoNI CCTnn'n_nnAGG Cuts at: 0 445 2328 Size: 445 1883 EcoO109I rG'GnC_Cy Cuts at: 0 245 501 2328 Size: 245 256 1827 Fragments arranged by size: 1827 256 245 EcoRV GAT'ATC Cuts at: 0 808 1824 2328 Size: 808 1016 504 Fragments arranged by size: 1016 808 504 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 353 385 1581 1613 1618 1650 2328 Size: 353 32 1196 32 5 32 678 Fragments arranged by size: 1196 678 353 32 32 32 5 HincII GTy'rAC Cuts at: 0 674 2328 Size: 674 1654 HindIII A'AGCT_T Cuts at: 0 1069 2328 Size: 1069 1259 HpaI GTT'AAC Cuts at: 0 674 2328 Size: 674 1654 MfeI C'AATT_G Cuts at: 0 709 2328 Size: 709 1619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1792 2328 Size: 1792 536 MscI TGG'CCA Cuts at: 0 230 1709 2328 Size: 230 1479 619 Fragments arranged by size: 1479 619 230 MslI CAynn'nnrTG Cuts at: 0 488 1907 2238 2328 Size: 488 1419 331 90 Fragments arranged by size: 1419 488 331 90 MspA1I CmG'CkG Cuts at: 0 768 2328 Size: 768 1560 NcoI C'CATG_G Cuts at: 0 483 2328 Size: 483 1845 NdeI CA'TA_TG Cuts at: 0 1800 2328 Size: 1800 528 NsiI A_TGCA'T Cuts at: 0 1250 2328 Size: 1250 1078 NspI r_CATG'y Cuts at: 0 439 1252 2284 2328 Size: 439 813 1032 44 Fragments arranged by size: 1032 813 439 44 PciI A'CATG_T Cuts at: 0 2280 2328 Size: 2280 48 PflMI CCAn_nnn'nTGG Cuts at: 0 1922 2328 Size: 1922 406 PfoI T'CCnGG_A Cuts at: 0 321 446 464 2328 Size: 321 125 18 1864 Fragments arranged by size: 1864 321 125 18 PpuMI rG'GwC_Cy Cuts at: 0 501 2328 Size: 501 1827 PsiI TTA'TAA Cuts at: 0 1004 2328 Size: 1004 1324 PstI C_TGCA'G Cuts at: 0 1288 2328 Size: 1288 1040 SanDI GG'GwC_CC Cuts at: 0 501 2328 Size: 501 1827 ScaI AGT'ACT Cuts at: 0 1311 1375 2328 Size: 1311 64 953 Fragments arranged by size: 1311 953 64 SfcI C'TryA_G Cuts at: 0 336 556 1231 1254 1284 1886 2328 Size: 336 220 675 23 30 602 442 Fragments arranged by size: 675 602 442 336 220 30 23 SphI G_CATG'C Cuts at: 0 439 1252 2328 Size: 439 813 1076 Fragments arranged by size: 1076 813 439 SspI AAT'ATT Cuts at: 0 801 1300 2328 Size: 801 499 1028 Fragments arranged by size: 1028 801 499 StyI C'CwwG_G Cuts at: 0 483 512 1758 2328 Size: 483 29 1246 570 Fragments arranged by size: 1246 570 483 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 148 1225 1762 1771 2328 Size: 148 1077 537 9 557 Fragments arranged by size: 1077 557 537 148 9 TatI w'GTAC_w Cuts at: 0 1309 1373 1465 2328 Size: 1309 64 92 863 Fragments arranged by size: 1309 863 92 64 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2192 2224 2328 Size: 2192 32 104 Fragments arranged by size: 2192 104 32 XbaI T'CTAG_A Cuts at: 0 117 2328 Size: 117 2211 XmnI GAAnn'nnTTC Cuts at: 0 1651 2328 Size: 1651 677 Enzymes that do cut: AccI AclI AcuI AflIII AleI AlfI ApaLI ApoI BanI BanII BbsI BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI PpuMI PsiI PstI SanDI ScaI SfcI SphI SspI StyI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglI BlpI BmtI Bpu10I BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI FspAI HaeII KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI Tth111I XcmI XhoI XmaI ZraI