Restriction map for
pFN21AE6769
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FseI
FspI HaeII HgaI Hin1I HindIII
KasI MamI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 1451 GGTACC G^GTACC
AlwNI 114 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 1411 GGCGCGCC GG^CGCGCC
AseI 883 ATTAAT AT^TAAT
AsnI 883 ATTAAT AT^TAAT
Asp718I 1451 GGTACC G^GTACC
AspHI 148 GAGCTC G[AT]GC[AT]^C
BanII 148 GAGCTC G[GA]GC[CT]^C
BbsI 820 GTCTTC GAAGAC(2/6)
BcnI 1353 CCCGG CC^[GC]GG
BfaI 332 CTAG C^TAG
BlpI 123 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTCAGC GC^T[ACGT]AGC
BpuAI 820 GTCTTC GAAGAC(2/6)
BsaI 933 GGTCTC GGTCTC(1/5)
BsaWI 1544 ACCGGA [AT]^CCGG[AT]
BsiHKAI 148 GAGCTC G[AT]GC[AT]^C
BsmFI 776 GGGAC GGGAC(10/14)
BsmI 423 GAATGC GAATGC(1/-1)
BspMI 1421 ACCTGC ACCTGC(4/8)
BsrDI 1151 GCAATG GCAATG(2/0)
BssHII 1412 GCGCGC G^CGCGC
BstAPI 694 GCAAGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1087 GGTCACC G^GT[ACGT]ACC
BstF5I 755 CATCC GGATG(2/0)
BstPI 1087 GGTCACC G^GT[ACGT]ACC
BstYI 738 GGATCT [GA]^GATC[CT]
CelII 123 GCTCAGC GC^T[ACGT]AGC
DraI 1522 TTTAAA TTT^AAA
DraII 404 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 552 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1338 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 404 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1087 GGTCACC G^GT[ACGT]ACC
EcoT22I 752 ATGCAT ATGCA^T
FokI 755 CATCC GGATG(9/13)
HincII 460 GTTAAC GT[CT]^[GA]AC
HindII 460 GTTAAC GT[CT]^[GA]AC
HpaI 460 GTTAAC GTT^AAC
KpnI 1451 GGTACC GGTAC^C
KspI 28 CCGCGG CCGC^GG
MaeI 332 CTAG C^TAG
MaeII 1472 ACGT A^CGT
MfeI 965 CAATTG C^AATTG
MflI 738 GGATCT [GA]^GATC[CT]
MunI 965 CAATTG C^AATTG
NciI 1353 CCCGG CC^[GC]GG
NdeI 1146 CATATG CA^TATG
NsiI 752 ATGCAT ATGCA^T
PleI 11 GACTC GAGTC(4/5)
PshBI 883 ATTAAT AT^TAAT
SacI 148 GAGCTC GAGCT^C
SacII 28 CCGCGG CCGC^GG
SexAI 1447 ACCTGGT A^CC[AT]GGT
SmlI 290 CTCAAG C^T[CT][GA]AG
SphI 128 GCATGC GCATG^C
StuI 137 AGGCCT AGG^CCT
XbaI 331 TCTAGA T^CTAGA
XhoII 738 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 738 GGATC GGATC(4/5)
925 GGATC
Asp700I 216 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
310 GAACATCTTC
BalI 2 TGGCCA TGG^CCA
35 TGGCCA
BclI 410 TGATCA T^GATCA
1188 TGATCA
BseRI 52 CTCCTC GAGGAG(10/8)
55 CTCCTC
BsmAI 1216 GAGAC GTCTC(1/5)
934 GTCTC
BsrI 1015 ACTGG ACTGG(1/-1)
1455 CCAGT
EarI 211 GAAGAG CTCTTC(1/4)
321 GAAGAG
FbaI 410 TGATCA T^GATCA
1188 TGATCA
HapII 1354 CCGG C^CGG
1545 CCGG
HpaII 1354 CCGG C^CGG
1545 CCGG
Ksp632I 211 GAAGAG CTCTTC(1/4)
321 GAAGAG
MluNI 2 TGGCCA TGG^CCA
35 TGGCCA
MscI 2 TGGCCA TGG^CCA
35 TGGCCA
MspA1I 28 CCGCGG C[AC]G^C[GT]G
1363 CCGCTG
MspI 1354 CCGG C^CGG
1545 CCGG
NspI 128 GCATGC [GA]CATG^[CT]
638 ACATGC
PflMI 168 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1461 CCATTCCTTGG
TaqI 496 TCGA T^CGA
1030 TCGA
TfiI 498 GATTC G^A[AT]TC
1032 GAATC
TthHB8I 496 TCGA T^CGA
1030 TCGA
Van91I 168 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1461 CCATTCCTTGG
XmnI 216 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
310 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaII BanI BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I Tsp509I TspRI