(Circular) (Six-base) MAPSORT of: pFN21AE6769.seq Check: 7112 from: 1 to: 6307 pFN21AE6769 6307 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3435 3435 Size: 6307 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3650 1937 Size: 1713 4594 Acc65I G'GTAC_C Cuts at: 3456 3628 3456 Size: 172 6135 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 3288 4370 5754 6022 1632 Size: 713 943 1082 1384 268 1917 Fragments arranged by size: 1917 1384 1082 943 713 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 5206 6183 1203 Size: 4003 977 1327 Fragments arranged by size: 4003 1327 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6307 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2445 1184 Size: 1261 5046 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2007 2039 2007 Size: 32 6275 AlwNI CAG_nnn'CTG Cuts at: 2124 4050 5622 2124 Size: 1926 1572 2809 Fragments arranged by size: 2809 1926 1572 ApaLI G'TGCA_C Cuts at: 4364 5520 4364 Size: 1156 5151 ApoI r'AATT_y Cuts at: 1493 2222 2267 2873 2946 3616 3842 3994 6103 Size: 729 45 606 73 670 226 152 2109 Cuts at: 6103 1493 Size: 1697 Fragments arranged by size: 2109 1697 729 670 606 226 152 73 45 AscI GG'CGCG_CC Cuts at: 3417 3417 Size: 6307 AseI AT'TA_AT Cuts at: 160 1032 2889 4861 160 Size: 872 1857 1972 1606 Fragments arranged by size: 1972 1857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 3632 1870 Size: 73 1689 4545 Fragments arranged by size: 4545 1689 73 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 2421 3456 3628 5083 Size: 325 217 627 317 317 1035 172 1455 Cuts at: 5083 618 Size: 1842 Fragments arranged by size: 1842 1455 1035 627 325 317 317 217 172 BanII G_rGCy'C Cuts at: 1391 2157 3626 1391 Size: 766 1469 4072 Fragments arranged by size: 4072 1469 766 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2818 928 Size: 971 919 4417 Fragments arranged by size: 4417 971 919 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4498 4532 1903 Size: 34 2561 34 3678 Fragments arranged by size: 3678 2561 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4199 5409 4199 Size: 1210 5097 BclI T'GATC_A Cuts at: 1548 2415 3193 1548 Size: 867 778 4662 Fragments arranged by size: 4662 867 778 BfrBI ATG'CAT Cuts at: 2759 2759 Size: 6307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4918 136 Size: 107 122 71 631 605 3246 1525 Fragments arranged by size: 3246 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6302 6302 Size: 6307 BlpI GC'TnA_GC Cuts at: 2129 3724 2129 Size: 1595 4712 Bme1580I G_kGCm'C Cuts at: 1790 2109 2558 4368 5524 1790 Size: 319 449 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 449 319 BmgBI CAC'GTC Cuts at: 1318 3478 1318 Size: 2160 4147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4038 4996 442 Size: 416 53 83 399 24 2621 958 1753 Fragments arranged by size: 2621 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2003 2919 3255 3322 4967 1239 Size: 764 916 336 67 1645 2579 Fragments arranged by size: 2579 1645 916 764 336 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2280 4432 5297 5595 2280 Size: 2152 865 298 2992 Fragments arranged by size: 2992 2152 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2944 4970 882 Size: 617 1445 2026 2219 Fragments arranged by size: 2219 2026 1445 617 BsaAI yAC'GTr Cuts at: 493 1204 4098 493 Size: 711 2894 2702 Fragments arranged by size: 2894 2702 711 BsaBI GATnn'nnATC Cuts at: 6301 6301 Size: 6307 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4114 4496 5131 275 Size: 53 83 186 936 2581 382 635 1451 Fragments arranged by size: 2581 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3549 4740 5138 5412 5559 5970 3549 Size: 1191 398 274 147 411 3886 Fragments arranged by size: 3886 1191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2040 2070 3222 3252 1178 Size: 30 165 30 637 30 1152 30 4233 Fragments arranged by size: 4233 1152 637 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2051 1186 Size: 862 3 5442 Fragments arranged by size: 5442 862 3 BseYI C'CCAG_C Cuts at: 2284 5510 6006 2284 Size: 3226 496 2585 Fragments arranged by size: 3226 2585 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5734 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4518 5546 1540 Size: 275 186 1756 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 4368 4453 5524 2157 Size: 2211 85 1071 2940 Fragments arranged by size: 2940 2211 1071 85 BsmI GAATG_Cn' Cuts at: 2434 3818 3911 2434 Size: 1384 93 4830 Fragments arranged by size: 4830 1384 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2157 2558 3626 4368 4453 5524 Size: 399 319 48 401 1068 742 85 1071 Cuts at: 5524 1391 Size: 2174 Fragments arranged by size: 2174 1071 1068 742 401 399 319 85 48 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3435 3662 844 Size: 2591 227 3489 Fragments arranged by size: 3489 2591 227 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3163 4802 4976 65 Size: 1183 1915 1639 174 1396 Fragments arranged by size: 1915 1639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4951 5138 1700 Size: 110 3141 187 2869 Fragments arranged by size: 3141 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 3417 1925 Size: 1492 4815 BssSI C'ACGA_G Cuts at: 4367 5379 4367 Size: 1012 5295 BstAPI GCAn_nnn'nTGC Cuts at: 794 2705 794 Size: 1911 4396 BstBI TT'CG_AA Cuts at: 5111 5111 Size: 6307 BstEII G'GTnAC_C Cuts at: 1184 3092 1184 Size: 1908 4399 BstYI r'GATC_y Cuts at: 1972 2743 3637 4004 4389 4406 5858 5944 5956 Size: 771 894 367 385 17 1452 86 12 Cuts at: 5956 6302 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 894 771 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2033 2252 3133 3168 513 Size: 552 939 29 219 881 35 3652 Fragments arranged by size: 3652 939 881 552 219 35 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5568 Fragments arranged by size: 5568 683 56 BtsI GCAGTG_nn' Cuts at: 3823 4617 4637 3823 Size: 794 20 5493 Fragments arranged by size: 5493 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 3529 3611 3529 Size: 82 6225 DraIII CAC_nnn'GTG Cuts at: 2562 2562 Size: 6307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2040 3378 Size: 54 1425 50 275 18 177 33 1338 Cuts at: 3378 3495 3754 4643 6272 8 Size: 117 259 889 1629 43 Fragments arranged by size: 1629 1425 1338 889 275 259 177 117 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 2321 4237 1353 Size: 858 110 1916 3423 Fragments arranged by size: 3423 1916 858 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4894 5268 5414 387 Size: 1064 138 3305 374 146 1280 Fragments arranged by size: 3305 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2155 2155 Size: 6307 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2003 2345 2919 3255 3288 3322 4370 Size: 393 371 342 574 336 33 34 1048 Cuts at: 4370 4967 5754 6022 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1048 787 597 574 393 371 342 336 268 34 33 EcoNI CCTnn'n_nnAGG Cuts at: 3347 3347 Size: 6307 EcoO109I rG'GnC_Cy Cuts at: 2410 2410 Size: 6307 EcoRI G'AATT_C Cuts at: 3616 3994 3616 Size: 378 5929 FspI TGC'GCA Cuts at: 4813 4813 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 5454 1678 Size: 3776 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 2922 2954 4948 Size: 32 135 32 468 32 850 32 1994 Cuts at: 4948 4980 5022 5054 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1994 850 468 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2467 3651 3897 669 Size: 988 281 529 1184 246 3079 Fragments arranged by size: 3079 1184 988 529 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6307 HpaI GTT'AAC Cuts at: 2467 3897 2467 Size: 1430 4877 KpnI G_GTAC'C Cuts at: 3460 3632 3460 Size: 172 6135 MfeI C'AATT_G Cuts at: 2970 3906 2970 Size: 936 5371 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5421 5605 1305 Size: 4116 184 2007 Fragments arranged by size: 4116 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2042 10 Size: 54 1425 343 177 33 4275 Fragments arranged by size: 4275 1425 343 177 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2427 2764 3173 4265 4624 4783 518 Size: 648 1261 337 409 1092 359 159 2042 Fragments arranged by size: 2042 1261 1092 648 409 359 337 159 MspA1I CmG'CkG Cuts at: 1606 2035 3370 3723 4400 5548 5793 1606 Size: 429 1335 353 677 1148 245 2120 Fragments arranged by size: 2120 1335 1148 677 429 353 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2252 3133 3168 513 Size: 552 939 248 881 35 3652 Fragments arranged by size: 3652 939 881 552 248 35 NdeI CA'TA_TG Cuts at: 387 3152 387 Size: 2765 3542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NsiI A_TGCA'T Cuts at: 2761 2761 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 2137 2647 3665 5210 1132 Size: 97 908 510 1018 1545 2229 Fragments arranged by size: 2229 1545 1018 908 510 97 PasI CC'CwG_GG Cuts at: 1348 2076 1348 Size: 728 5579 PciI A'CATG_T Cuts at: 5206 5206 Size: 6307 PflMI CCAn_nnn'nTGG Cuts at: 1108 2179 3472 1108 Size: 1071 1293 3943 Fragments arranged by size: 3943 1293 1071 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2633 2665 2633 Size: 32 6275 PstI C_TGCA'G Cuts at: 830 3659 830 Size: 2829 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 SacI G_AGCT'C Cuts at: 2157 2157 Size: 6307 SacII CC_GC'GG Cuts at: 2036 2036 Size: 6307 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 4555 6267 4555 Size: 1712 4595 SexAI A'CCwGG_T Cuts at: 3452 3452 Size: 6307 SfcI C'TryA_G Cuts at: 826 1045 3655 4790 5471 5662 826 Size: 219 2610 1135 681 191 1471 Fragments arranged by size: 2610 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 3634 1872 Size: 1762 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2295 4411 5312 5574 820 Size: 197 926 352 2116 901 262 1553 Fragments arranged by size: 2116 1553 926 901 352 262 197 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6307 SphI G_CATG'C Cuts at: 1132 2137 3665 1132 Size: 1005 1528 3774 Fragments arranged by size: 3774 1528 1005 SspI AAT'ATT Cuts at: 5 52 4231 5 Size: 47 4179 2081 Fragments arranged by size: 4179 2081 47 StuI AGG'CCT Cuts at: 1517 2144 1517 Size: 627 5680 StyI C'CwwG_G Cuts at: 513 1065 2004 2252 3133 3168 3471 3746 513 Size: 552 939 248 881 35 303 275 3074 Fragments arranged by size: 3074 939 881 552 303 275 248 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3134 3379 4334 4351 4504 4689 5134 Size: 1255 1554 245 955 17 153 185 445 Cuts at: 5134 325 Size: 1498 Fragments arranged by size: 1554 1498 1255 955 445 245 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4553 6265 96 Size: 274 80 33 51 1443 2576 1712 138 Fragments arranged by size: 2576 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2548 2580 3222 3254 6267 6299 2548 Size: 32 642 32 3013 32 2556 Fragments arranged by size: 3013 2556 642 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6307 XbaI T'CTAG_A Cuts at: 2336 3643 2336 Size: 1307 5000 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 3632 1870 Size: 1762 4545 XmnI GAAnn'nnTTC Cuts at: 2225 2319 4436 2225 Size: 94 2117 4096 Fragments arranged by size: 4096 2117 94 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI