Restriction map for
pFN21AE6768
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvrII
BanI BbeI BbrPI BcgI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MluI MroI MvnI NarI
NciI NdeI NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AlwNI 350 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 859 GAGCTC G[AT]GC[AT]^C
AvaII 1476 GGACC G^G[AT]CC
AviII 1074 TGCGCA TGC^GCA
BamHI 1365 GGATCC G^GATCC
BbvCI 633 CCTCAGC CCTCAGC(-5/-2)
BbvI 185 GCTGC GCAGC(8/12)
BciVI 988 GGATAC GTATCC(6/5)
BclI 659 TGATCA T^GATCA
BpmI 76 CTGGAG CTGGAG(16/14)
BsaBI 1366 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 643 ACCGGA [AT]^CCGG[AT]
BseRI 96 GAGGAG GAGGAG(10/8)
BsgI 435 CTGCAC GTGCAG(16/14)
BsiHKAI 859 GAGCTC G[AT]GC[AT]^C
BspMI 85 ACCTGC ACCTGC(4/8)
BsrDI 594 CATTGC GCAATG(2/0)
BsrFI 518 GCCGGC [GA]^CCGG[CT]
BstYI 1365 GGATCC [GA]^GATC[CT]
CfoI 1075 GCGC GCG^C
Cfr10I 518 GCCGGC [GA]^CCGG[CT]
DraI 496 TTTAAA TTT^AAA
DraIII 1202 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 688 CCATGG C^C[GA][CT]GG
EcoT22I 388 ATGCAT ATGCA^T
FbaI 659 TGATCA T^GATCA
FspI 1074 TGCGCA TGC^GCA
HgaI 869 GACGC GACGC(5/10)
HhaI 1075 GCGC GCG^C
HinP1I 1075 GCGC G^CGC
MamI 1366 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 749 CAATTG C^AATTG
MflI 1365 GGATCC [GA]^GATC[CT]
MslI 594 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 749 CAATTG C^AATTG
NaeI 518 GCCGGC GCC^GGC
NcoI 688 CCATGG C^CATGG
NgoMIV 518 GCCGGC G^CCGGC
NsiI 388 ATGCAT ATGCA^T
PaeR7I 1502 CTCGAG C^TCGAG
PshAI 321 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 330 CTGCAG CTGCA^G
SacI 859 GAGCTC GAGCT^C
SapI 955 GAAGAGC GCTCTTC(1/4)
ScaI 506 AGTACT AGT^ACT
SmlI 1502 CTCGAG C^T[CT][GA]AG
StuI 1046 AGGCCT AGG^CCT
TseI 185 GCTGC G^C[AT]GC
XcmI 748 CCAATTGTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1502 CTCGAG C^TCGAG
XhoII 1365 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 135 ACATGT A^C[GA][CT]GT
722 ACATGT
AlwI 1366 GATCC GGATC(4/5)
1365 GGATC
AseI 487 ATTAAT AT^TAAT
1225 ATTAAT
AsnI 487 ATTAAT AT^TAAT
1225 ATTAAT
Asp700I 885 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1534 GAAAGTTTTC
AvaI 678 CTCGGG C^[CT]CG[GA]G
1502 CTCGAG
BbsI 820 GAAGAC GAAGAC(2/6)
1387 GTCTTC
BpuAI 820 GAAGAC GAAGAC(2/6)
1387 GTCTTC
BsmFI 692 GGGAC GGGAC(10/14)
1475 GGGAC
BsoBI 678 CTCGGG C^[CT]CG[GA]G
1502 CTCGAG
Bsp1407I 1055 TGTACA T^GTACA
1509 TGTACA
BspLU11I 135 ACATGT A^CATGT
722 ACATGT
BsrGI 1055 TGTACA T^GTACA
1509 TGTACA
BstF5I 532 CATCC GGATG(2/0)
652 GGATG
BstXI 608 CCAGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1432 CCAGGAAAATGG
Cac8I 518 GCCGGC GC[ACGT]^[ACGT]GC
607 GCCAGC
DpnII 660 GATC ^GATC
1366 GATC
DrdI 550 GACAAGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
967 GACATCATTGTC
EarI 394 GAAGAG CTCTTC(1/4)
955 GAAGAG
Fnu4HI 38 GCGGC GC^[ACGT]GC
185 GCTGC
FokI 532 CATCC GGATG(9/13)
652 GGATG
HapII 519 CCGG C^CGG
644 CCGG
HpaII 519 CCGG C^CGG
644 CCGG
ItaI 38 GCGGC GC^[ACGT]GC
185 GCTGC
Ksp632I 394 GAAGAG CTCTTC(1/4)
955 GAAGAG
MboI 660 GATC ^GATC
1366 GATC
MspI 519 CCGG C^CGG
644 CCGG
NdeII 660 GATC ^GATC
1366 GATC
NspI 135 ACATGT [GA]CATG^[CT]
722 ACATGT
PshBI 487 ATTAAT AT^TAAT
1225 ATTAAT
PvuII 183 CAGCTG CAG^CTG
832 CAGCTG
Sau3AI 660 GATC ^GATC
1366 GATC
SspBI 1055 TGTACA T^GTACA
1509 TGTACA
TaqI 1170 TCGA T^CGA
1503 TCGA
Tsp45I 382 GTCAC ^GT[GC]AC
754 GTGAC
TthHB8I 1170 TCGA T^CGA
1503 TCGA
XmnI 885 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1534 GAAAGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BalI BanII BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BspHI BsrI
BssKI BstNI Cfr13I DdeI EaeI
EcoRII EcoT14I HaeIII HinfI HphI
MaeIII MboII MluNI MnlI MscI
MseI MspA1I MvaI MwoI NlaIII
NlaIV PleI RcaI RsaI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I Tsp509I TspRI