(Circular) (Six-base) MAPSORT of: pFN21AE6768.seq Check: 268 from: 1 to: 6301 pFN21AE6768 6301 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2099 2099 Size: 6301 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4364 5748 6016 1632 Size: 2732 1384 268 1917 Fragments arranged by size: 2732 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2140 2727 5200 6177 1203 Size: 937 587 2473 977 1327 Fragments arranged by size: 2473 1327 977 937 587 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2618 2650 2690 2722 2618 Size: 32 40 32 6197 Fragments arranged by size: 6197 40 32 32 AlwNI CAG_nnn'CTG Cuts at: 2360 4044 5616 2360 Size: 1684 1572 3045 Fragments arranged by size: 3045 1684 1572 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 2254 2584 2737 3610 3836 3988 6097 1493 Size: 761 330 153 873 226 152 2109 1697 Fragments arranged by size: 2109 1697 873 761 330 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 2493 3231 4855 160 Size: 872 1461 738 1624 1606 Fragments arranged by size: 1624 1606 1461 872 738 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2683 3507 3626 1870 Size: 73 740 824 119 4545 Fragments arranged by size: 4545 824 740 119 73 BamHI G'GATC_C Cuts at: 3370 3631 3998 3370 Size: 261 367 5673 Fragments arranged by size: 5673 367 261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3622 5077 618 Size: 325 217 627 1835 1455 1842 Fragments arranged by size: 1842 1835 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2868 3134 3620 1391 Size: 697 780 266 486 4072 Fragments arranged by size: 4072 780 697 486 266 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2832 3385 928 Size: 971 933 553 3844 Fragments arranged by size: 3844 971 933 553 BbvCI CC'TCA_GC Cuts at: 2639 2639 Size: 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2987 4193 5403 2987 Size: 1206 1210 3885 Fragments arranged by size: 3885 1210 1206 BclI T'GATC_A Cuts at: 1548 2664 1548 Size: 1116 5185 BfrBI ATG'CAT Cuts at: 2395 2395 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 4362 5518 1790 Size: 2572 1156 2573 Fragments arranged by size: 2573 2572 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2895 3249 3469 Size: 416 53 83 399 24 1478 354 220 Cuts at: 3469 4032 4990 442 Size: 563 958 1753 Fragments arranged by size: 1753 1478 958 563 416 399 354 220 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2180 2180 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 4961 1239 Size: 863 2859 2579 Fragments arranged by size: 2859 2579 863 Bpu10I CC'TnA_GC Cuts at: 2639 2639 Size: 6301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4426 5291 5589 4426 Size: 865 298 5138 Fragments arranged by size: 5138 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4964 882 Size: 617 3465 2219 Fragments arranged by size: 3465 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4092 493 Size: 711 2888 2702 Fragments arranged by size: 2888 2702 711 BsaBI GATnn'nnATC Cuts at: 3375 6295 3375 Size: 2920 3381 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2648 4734 5132 5406 5553 5964 2648 Size: 2086 398 274 147 411 2985 Fragments arranged by size: 2985 2086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 1178 Size: 30 165 30 619 30 5427 Fragments arranged by size: 5427 619 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 1186 Size: 930 5371 BseYI C'CCAG_C Cuts at: 2033 5504 6000 2033 Size: 3471 496 2334 Fragments arranged by size: 3471 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2425 1163 Size: 573 689 5039 Fragments arranged by size: 5039 689 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2868 4362 4447 5518 2868 Size: 1494 85 1071 3651 Fragments arranged by size: 3651 1494 1071 85 BsmI GAATG_Cn' Cuts at: 3812 3905 3812 Size: 93 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2868 3134 3620 4362 4447 5518 Size: 399 298 780 266 486 742 85 1071 Cuts at: 5518 1391 Size: 2174 Fragments arranged by size: 2174 1071 780 742 486 399 298 266 85 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 2709 3216 3468 4190 5920 2709 Size: 507 252 722 1730 3090 Fragments arranged by size: 3090 1730 722 507 252 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 3656 844 Size: 1255 1557 3489 Fragments arranged by size: 3489 1557 1255 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2598 4796 4970 65 Size: 1183 1350 2198 174 1396 Fragments arranged by size: 2198 1396 1350 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2523 4945 5132 1700 Size: 110 713 2422 187 2869 Fragments arranged by size: 2869 2422 713 187 110 BsrGI T'GTAC_A Cuts at: 96 3060 3514 96 Size: 2964 454 2883 Fragments arranged by size: 2964 2883 454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 2620 3444 2620 Size: 824 5477 BstYI r'GATC_y Cuts at: 1972 3370 3631 3998 4383 4400 5852 5938 5950 Size: 1398 261 367 385 17 1452 86 12 Cuts at: 5950 6296 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1398 385 367 346 261 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2693 513 Size: 552 939 689 4121 Fragments arranged by size: 4121 939 689 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2121 487 Size: 683 56 895 4667 Fragments arranged by size: 4667 895 683 56 BtsI GCAGTG_nn' Cuts at: 2344 3817 4611 4631 2344 Size: 1473 794 20 4014 Fragments arranged by size: 4014 1473 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2077 2112 3146 3181 529 Size: 35 1513 35 1034 35 3649 Fragments arranged by size: 3649 1513 1034 35 35 35 DraI TTT'AAA Cuts at: 2503 3605 2503 Size: 1102 5199 DraIII CAC_nnn'GTG Cuts at: 3212 3212 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2561 2978 5308 809 Size: 507 1245 417 2330 1802 Fragments arranged by size: 2330 1802 1245 507 417 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2371 3046 Size: 54 1425 50 275 18 177 364 675 Cuts at: 3046 3102 3748 4637 6266 8 Size: 56 646 889 1629 43 Fragments arranged by size: 1629 1425 889 675 646 364 275 177 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2955 4231 1353 Size: 1041 561 1276 3423 Fragments arranged by size: 3423 1276 1041 561 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2639 4888 5262 5408 387 Size: 1064 138 1050 2249 374 146 1280 Fragments arranged by size: 2249 1280 1064 1050 374 146 138 EcoICRI GAG'CTC Cuts at: 2866 2866 Size: 6301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 4364 4961 5748 6016 1239 Size: 393 470 2262 597 787 268 1524 Fragments arranged by size: 2262 1524 787 597 470 393 268 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3088 3088 Size: 6301 FspI TGC'GCA Cuts at: 3081 4807 3081 Size: 1726 4575 HaeII r_GCGC'y Cuts at: 1678 5448 1678 Size: 3770 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2649 2681 2865 Size: 32 135 32 450 32 595 32 184 Cuts at: 2865 2897 3512 3544 4942 4974 5016 5048 1373 Size: 32 615 32 1398 32 42 32 2626 Fragments arranged by size: 2626 1398 615 595 450 184 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3645 3891 669 Size: 988 281 1707 246 3079 Fragments arranged by size: 3079 1707 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 2754 3900 2754 Size: 1146 5155 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 5415 5599 1305 Size: 903 3207 184 2007 Fragments arranged by size: 3207 2007 903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2373 3048 3104 10 Size: 54 1425 343 177 364 675 56 3207 Fragments arranged by size: 3207 1425 675 364 343 177 56 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 4259 4618 4777 518 Size: 648 1437 1656 359 159 2042 Fragments arranged by size: 2042 1656 1437 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2190 2839 3717 4394 5542 5787 1606 Size: 437 147 649 878 677 1148 245 2120 Fragments arranged by size: 2120 1148 878 677 649 437 245 147 NaeI GCC'GGC Cuts at: 2525 2525 Size: 6301 NcoI C'CATG_G Cuts at: 513 1065 2004 2693 513 Size: 552 939 689 4121 Fragments arranged by size: 4121 939 689 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NgoMIV G'CCGG_C Cuts at: 2523 2523 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NsiI A_TGCA'T Cuts at: 2397 2397 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2144 2731 3659 5204 1132 Size: 97 915 587 928 1545 2229 Fragments arranged by size: 2229 1545 928 915 587 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 2140 2727 5200 2140 Size: 587 2473 3241 Fragments arranged by size: 3241 2473 587 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PfoI T'CCnGG_A Cuts at: 2265 2265 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2330 1613 Size: 717 5584 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspXI vC'TCGA_Gb Cuts at: 3507 3507 Size: 6301 PstI C_TGCA'G Cuts at: 830 2339 3653 830 Size: 1509 1314 3478 Fragments arranged by size: 3478 1509 1314 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2190 2839 2190 Size: 649 5652 SacI G_AGCT'C Cuts at: 2868 2868 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SapI GCTCTTCn'nnn_ Cuts at: 2955 2955 Size: 6301 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 2513 4549 6261 2513 Size: 2036 1712 2553 Fragments arranged by size: 2553 2036 1712 SfcI C'TryA_G Cuts at: 826 1045 2335 3027 3144 3649 4784 5465 5656 Size: 219 1290 692 117 505 1135 681 191 Cuts at: 5656 826 Size: 1471 Fragments arranged by size: 1471 1290 1135 692 681 505 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3507 4405 5306 5568 820 Size: 197 926 1564 898 901 262 1553 Fragments arranged by size: 1564 1553 926 901 898 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 3053 1517 Size: 1536 4765 StyI C'CwwG_G Cuts at: 513 1065 2004 2113 2693 3126 3740 513 Size: 552 939 109 580 433 614 3074 Fragments arranged by size: 3074 939 614 580 552 433 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2650 3498 4328 4345 4498 4683 5128 Size: 1255 1070 848 830 17 153 185 445 Cuts at: 5128 325 Size: 1498 Fragments arranged by size: 1498 1255 1070 848 830 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2511 3060 3514 Size: 274 80 33 51 1443 534 549 454 Cuts at: 3514 4547 6259 96 Size: 1033 1712 138 Fragments arranged by size: 1712 1443 1033 549 534 454 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2760 2760 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 3507 1943 Size: 1564 4737 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 2894 3543 4430 2894 Size: 649 887 4765 Fragments arranged by size: 4765 887 649 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI