################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BbrPI BcgI BciVI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 844 GACGTC GACGT^C AcsI 949 GAATTT [GA]^AATT[CT] AfaI 879 GTAC GT^AC Aor51HI 1142 AGCGCT AGC^GCT ApaI 70 GGGCCC GGGCC^C ApoI 949 GAATTT [GA]^AATT[CT] AvrII 267 CCTAGG C^CTAGG BbeI 791 GGCGCC GGCGC^C BfaI 268 CTAG C^TAG BglII 92 AGATCT A^GATCT BlnI 267 CCTAGG C^CTAGG BsmAI 773 GTCTC GTCTC(1/5) BsrBI 141 GAGCGG CCGCTC(-3/-3) BstXI 998 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 514 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1178 CGGCCG C^GGCCG EarI 666 CTCTTC CTCTTC(1/4) EclXI 1178 CGGCCG C^GGCCG Eco47III 1142 AGCGCT AGC^GCT Eco52I 1178 CGGCCG C^GGCCG HincII 847 GTCAAC GT[CT]^[GA]AC HindII 847 GTCAAC GT[CT]^[GA]AC HphI 1013 TCACC GGTGA(8/7) KasI 791 GGCGCC G^GCGCC Ksp632I 666 CTCTTC CTCTTC(1/4) MaeI 268 CTAG C^TAG NarI 791 GGCGCC GG^CGCC PaeR7I 637 CTCGAG C^TCGAG PvuI 18 CGATCG CGAT^CG RsaI 879 GTAC GT^AC SacI 979 GAGCTC GAGCT^C SfiI 71 GGCCCATCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 791 GGCGCC GGC^GCC TaqI 638 TCGA T^CGA TfiI 456 GAATC G^A[AT]TC Tsp509I 950 AATT ^AATT TthHB8I 638 TCGA T^CGA XcmI 995 CCACCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 637 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 583 CGCG CG^CG 788 CGCG AflIII 59 ACGTGT A^C[GA][CT]GT 919 ACGTGT AlwNI 119 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 736 CAGGGGCTG BalI 50 TGGCCA TGG^CCA 992 TGGCCA BanI 791 GGCGCC G^G[CT][GA]CC 1078 GGTGCC BbsI 435 GAAGAC GAAGAC(2/6) 933 GAAGAC BbvCI 1193 CCTCAGC CCTCAGC(-5/-2) 684 GCTGAGG BpuAI 435 GAAGAC GAAGAC(2/6) 933 GAAGAC BseRI 748 GAGGAG GAGGAG(10/8) 958 GAGGAG BsrI 1103 ACTGG ACTGG(1/-1) 1160 CCAGT BstUI 583 CGCG CG^CG 788 CGCG BstYI 92 AGATCT [GA]^GATC[CT] 578 AGATCC DrdI 376 GACCCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 862 GACCCCTACGTC DsaI 353 CCATGG C^C[GA][CT]GG 839 CCATGG HgaI 1129 GACGC GACGC(5/10) 658 GCGTC MflI 92 AGATCT [GA]^GATC[CT] 578 AGATCC MluNI 50 TGGCCA TGG^CCA 992 TGGCCA MscI 50 TGGCCA TGG^CCA 992 TGGCCA MseI 626 TTAA T^TAA 953 TTAA MslI 54 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1102 CACTGGAGTG MvnI 583 CGCG CG^CG 788 CGCG NcoI 353 CCATGG C^CATGG 839 CCATGG PpuMI 120 AGGACCT [GA]G^G[AT]CC[CT] 473 AGGACCT PstI 191 CTGCAG CTGCA^G 1117 CTGCAG SmaI 67 CCCGGG CCC^GGG 110 CCCGGG Tru9I 626 TTAA T^TAA 953 TTAA XhoII 92 AGATCT [GA]^GATC[CT] 578 AGATCC XmaCI 67 CCCGGG C^CCGGG 110 CCCGGG XmaI 67 CCCGGG C^CCGGG 110 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcyI AluI AlwI AocI AspHI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstAPI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TseI Tsp45I TspRI