(Circular) (Six-base) MAPSORT of: pFN21AE6696.seq Check: 5898 from: 1 to: 5905 pFN21AE6696 5905 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2853 3715 278 Size: 53 83 186 936 1317 862 2468 Fragments arranged by size: 2468 1317 936 862 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 2732 3968 5352 5620 1632 Size: 519 581 1236 1384 268 1917 Fragments arranged by size: 1917 1384 1236 581 519 268 AfeI AGC'GCT Cuts at: 3149 3149 Size: 5905 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2064 2924 4804 5781 1203 Size: 861 860 1880 977 1327 Fragments arranged by size: 1880 1327 977 861 860 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 3111 1166 Size: 1945 3960 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2129 2746 3648 5220 2129 Size: 617 902 1572 2814 Fragments arranged by size: 2814 1572 902 617 ApaI G_GGCC'C Cuts at: 2079 2079 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2954 3214 3440 3592 5701 1493 Size: 1461 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 1461 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2115 2642 3087 3230 1870 Size: 73 129 43 527 445 143 4545 Fragments arranged by size: 4545 527 445 143 129 73 43 AvrII C'CTAG_G Cuts at: 2272 2272 Size: 5905 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2796 3083 3226 4681 618 Size: 325 217 627 1009 287 143 1455 1842 Fragments arranged by size: 1842 1455 1009 627 325 287 217 143 BanII G_rGCy'C Cuts at: 1391 2079 2988 3193 3224 1391 Size: 688 909 205 31 4072 Fragments arranged by size: 4072 909 688 205 31 BbeI G_GCGC'C Cuts at: 2800 2800 Size: 5905 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2447 2945 928 Size: 971 548 498 3888 Fragments arranged by size: 3888 971 548 498 BbvCI CC'TCA_GC Cuts at: 2690 3199 2690 Size: 509 5396 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2019 2083 2420 Size: 107 122 71 631 605 347 64 337 Cuts at: 2420 2837 4516 136 Size: 417 1679 1525 Fragments arranged by size: 1679 1525 631 605 417 347 337 122 107 71 64 BglII A'GATC_T Cuts at: 2097 5900 2097 Size: 3803 2102 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2072 2079 2595 3088 3966 5122 1790 Size: 282 7 516 493 878 1156 2573 Fragments arranged by size: 2573 1156 878 516 493 282 7 BmgBI CAC'GTC Cuts at: 1318 2925 1318 Size: 1607 4298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2430 2771 3130 3164 4565 1239 Size: 1064 127 341 359 34 1401 2579 Fragments arranged by size: 2579 1401 1064 359 341 127 34 Bpu10I CC'TnA_GC Cuts at: 2528 2690 3199 2528 Size: 162 509 5234 Fragments arranged by size: 5234 509 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 2591 4030 4895 5193 2336 Size: 255 1439 865 298 3048 Fragments arranged by size: 3048 1439 865 298 255 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2663 2797 2850 3712 Size: 53 83 186 936 1130 134 53 862 Cuts at: 3712 4094 4729 275 Size: 382 635 1451 Fragments arranged by size: 1451 1130 936 862 635 382 186 134 83 53 53 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2294 2324 1178 Size: 30 165 30 891 30 4759 Fragments arranged by size: 4759 891 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2768 2978 1186 Size: 1582 210 4113 Fragments arranged by size: 4113 1582 210 BseYI C'CCAG_C Cuts at: 5108 5604 5108 Size: 496 5409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2299 2306 2381 1163 Size: 573 563 7 75 4687 Fragments arranged by size: 4687 573 563 75 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2026 2305 3186 3355 4116 5144 Size: 275 186 25 279 881 169 761 1028 Cuts at: 5144 1540 Size: 2301 Fragments arranged by size: 2301 1028 881 761 279 275 186 169 25 BsiHKAI G_wGCw'C Cuts at: 2052 2529 2988 3966 4051 5122 2052 Size: 477 459 978 85 1071 2835 Fragments arranged by size: 2835 1071 978 477 459 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2072 2079 2529 2595 2988 3088 Size: 399 262 20 7 450 66 393 100 Cuts at: 3088 3193 3224 3966 4051 5122 1391 Size: 105 31 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 393 262 105 100 85 66 31 20 7 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2148 3792 1394 Size: 429 325 1644 3507 Fragments arranged by size: 3507 1644 429 325 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 2404 2524 2746 794 Size: 1610 120 222 3953 Fragments arranged by size: 3953 1610 222 120 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstXI CCAn_nnnn'nTGG Cuts at: 3010 3010 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2097 2583 3235 3602 3987 4004 5456 5542 Size: 125 486 652 367 385 17 1452 86 Cuts at: 5542 5554 5900 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 652 486 385 367 346 125 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2223 2336 2888 2223 Size: 113 552 5240 Fragments arranged by size: 5240 552 113 BtgI C'CryG_G Cuts at: 513 1065 2358 2844 513 Size: 552 1293 486 3574 Fragments arranged by size: 3574 1293 552 486 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 3421 4215 4235 3421 Size: 794 20 5091 Fragments arranged by size: 5091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DraIII CAC_nnn'GTG Cuts at: 2524 2524 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2387 2873 4912 809 Size: 507 1071 486 2039 1802 Fragments arranged by size: 2039 1802 1071 507 486 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2761 2997 Size: 54 1425 50 275 18 225 706 236 Cuts at: 2997 3183 3352 4241 5870 8 Size: 186 169 889 1629 43 Fragments arranged by size: 1629 1425 889 706 275 236 225 186 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3183 3352 1537 Size: 275 1371 169 4090 Fragments arranged by size: 4090 1371 275 169 EarI CTCTTCn'nnn_ Cuts at: 1353 2677 3835 1353 Size: 1324 1158 3423 Fragments arranged by size: 3423 1324 1158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2081 2588 2744 4492 4866 5012 Size: 1064 138 492 507 156 1748 374 146 Cuts at: 5012 387 Size: 1280 Fragments arranged by size: 1748 1280 1064 507 492 374 156 146 138 EcoICRI GAG'CTC Cuts at: 2986 2986 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2303 2430 2732 2771 3130 3164 Size: 393 519 152 127 302 39 359 34 Cuts at: 3164 3968 4565 5352 5620 1239 Size: 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 519 393 359 302 268 152 127 39 34 EcoO109I rG'GnC_Cy Cuts at: 2126 2158 2347 2479 2126 Size: 32 189 132 5552 Fragments arranged by size: 5552 189 132 32 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2650 2690 2800 3151 5052 1678 Size: 972 40 110 351 1901 2531 Fragments arranged by size: 2531 1901 972 351 110 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2894 2926 4546 Size: 32 135 32 722 32 568 32 1620 Cuts at: 4546 4578 4620 4652 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1620 722 568 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2854 3249 3495 669 Size: 988 281 916 395 246 3079 Fragments arranged by size: 3079 988 916 395 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KasI G'GCGC_C Cuts at: 2796 2796 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2700 5019 5203 1305 Size: 1395 2319 184 2007 Fragments arranged by size: 2319 2007 1395 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 2999 10 Size: 54 1425 343 225 942 2916 Fragments arranged by size: 2916 1425 942 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 3111 3863 4222 4381 518 Size: 648 897 1048 752 359 159 2042 Fragments arranged by size: 2042 1048 897 752 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2493 2659 2684 3321 3998 5146 5391 1606 Size: 887 166 25 637 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 637 245 166 25 NarI GG'CG_CC Cuts at: 2797 2797 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 2358 2844 513 Size: 552 1293 486 3574 Fragments arranged by size: 3574 1293 552 486 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 3073 1348 Size: 1725 4180 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2043 2043 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2126 2479 2126 Size: 353 5552 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2075 2075 Size: 5905 PspXI vC'TCGA_Gb Cuts at: 2642 2642 Size: 5905 PstI C_TGCA'G Cuts at: 830 2200 3126 3257 830 Size: 1370 926 131 3478 Fragments arranged by size: 3478 1370 926 131 PvuI CG_AT'CG Cuts at: 2001 2026 2001 Size: 25 5880 SacI G_AGCT'C Cuts at: 2988 2988 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2196 2326 2785 3122 3253 4388 5069 Size: 219 1151 130 459 337 131 1135 681 Cuts at: 5069 5260 826 Size: 191 1471 Fragments arranged by size: 1471 1151 1135 681 459 337 219 191 131 130 SfiI GGCCn_nnn'nGGCC Cuts at: 2083 2083 Size: 5905 SfoI GGC'GCC Cuts at: 2798 2798 Size: 5905 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 2074 2117 3232 1872 Size: 202 43 1115 4545 Fragments arranged by size: 4545 1115 202 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 2606 2642 4009 4910 5172 Size: 197 926 408 255 36 1367 901 262 Cuts at: 5172 820 Size: 1553 Fragments arranged by size: 1553 1367 926 901 408 262 255 197 36 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2206 2272 2343 2358 2844 3344 513 Size: 552 1141 66 71 15 486 500 3074 Fragments arranged by size: 3074 1141 552 500 486 71 66 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3932 3949 4102 4287 4732 325 Size: 1255 2352 17 153 185 445 1498 Fragments arranged by size: 2352 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4151 5863 96 Size: 274 80 33 51 1443 2174 1712 138 Fragments arranged by size: 2174 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2446 2478 5865 5897 2446 Size: 32 3387 32 2454 Fragments arranged by size: 3387 2454 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3007 3007 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 2642 1943 Size: 699 5206 XmaI C'CCGG_G Cuts at: 1870 2072 2115 3230 1870 Size: 202 43 1115 4545 Fragments arranged by size: 4545 1115 202 43 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2851 3713 276 Size: 53 83 186 936 1317 862 2468 Fragments arranged by size: 2468 1317 936 862 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI SapI SexAI SrfI SwaI