(Linear) (Six-base) MAPSORT of: KIEE6696.seq Check: 1373 from: 1 to: 1200 KIEE6696.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AatII G_ACGT'C Cuts at: 0 848 1200 Size: 848 352 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 727 1200 Size: 146 581 473 Fragments arranged by size: 581 473 146 AfeI AGC'GCT Cuts at: 0 1144 1200 Size: 1144 56 AflIII A'CryG_T Cuts at: 0 59 919 1200 Size: 59 860 281 Fragments arranged by size: 860 281 59 AleI CACnn'nnGTG Cuts at: 0 1106 1200 Size: 1106 94 AlwNI CAG_nnn'CTG Cuts at: 0 124 741 1200 Size: 124 617 459 Fragments arranged by size: 617 459 124 ApaI G_GGCC'C Cuts at: 0 74 1200 Size: 74 1126 ApoI r'AATT_y Cuts at: 0 949 1200 Size: 949 251 AvaI C'yCGr_G Cuts at: 0 67 110 637 1082 1200 Size: 67 43 527 445 118 Fragments arranged by size: 527 445 118 67 43 AvrII C'CTAG_G Cuts at: 0 267 1200 Size: 267 933 BanI G'GyrC_C Cuts at: 0 791 1078 1200 Size: 791 287 122 Fragments arranged by size: 791 287 122 BanII G_rGCy'C Cuts at: 0 74 983 1188 1200 Size: 74 909 205 12 Fragments arranged by size: 909 205 74 12 BbeI G_GCGC'C Cuts at: 0 795 1200 Size: 795 405 BbsI GAAGACnn'nnnn_ Cuts at: 0 442 940 1200 Size: 442 498 260 Fragments arranged by size: 498 442 260 BbvCI CC'TCA_GC Cuts at: 0 685 1194 1200 Size: 685 509 6 Fragments arranged by size: 685 509 6 BglI GCCn_nnn'nGGC Cuts at: 0 14 78 415 832 1200 Size: 14 64 337 417 368 Fragments arranged by size: 417 368 337 64 14 BglII A'GATC_T Cuts at: 0 92 1200 Size: 92 1108 Bme1580I G_kGCm'C Cuts at: 0 67 74 590 1083 1200 Size: 67 7 516 493 117 Fragments arranged by size: 516 493 117 67 7 BmgBI CAC'GTC Cuts at: 0 920 1200 Size: 920 280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 425 766 1125 1159 1200 Size: 298 127 341 359 34 41 Fragments arranged by size: 359 341 298 127 41 34 Bpu10I CC'TnA_GC Cuts at: 0 523 685 1194 1200 Size: 523 162 509 6 Fragments arranged by size: 523 509 162 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 586 1200 Size: 331 255 614 Fragments arranged by size: 614 331 255 BsaHI Gr'CG_yC Cuts at: 0 658 792 845 1200 Size: 658 134 53 355 Fragments arranged by size: 658 355 134 53 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 289 319 1200 Size: 289 30 881 Fragments arranged by size: 881 289 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 763 973 1200 Size: 763 210 227 Fragments arranged by size: 763 227 210 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 301 376 1200 Size: 294 7 75 824 Fragments arranged by size: 824 294 75 7 BsiEI CG_ry'CG Cuts at: 0 21 300 1181 1200 Size: 21 279 881 19 Fragments arranged by size: 881 279 21 19 BsiHKAI G_wGCw'C Cuts at: 0 47 524 983 1200 Size: 47 477 459 217 Fragments arranged by size: 477 459 217 47 Bsp1286I G_dGCh'C Cuts at: 0 47 67 74 524 590 983 1083 1188 Size: 47 20 7 450 66 393 100 105 Cuts at: 1188 1200 Size: 12 Fragments arranged by size: 450 393 105 100 66 47 20 12 7 BsrBI CCG'CTC Cuts at: 0 143 1200 Size: 143 1057 BstAPI GCAn_nnn'nTGC Cuts at: 0 399 519 741 1200 Size: 399 120 222 459 Fragments arranged by size: 459 399 222 120 BstXI CCAn_nnnn'nTGG Cuts at: 0 1005 1200 Size: 1005 195 BstYI r'GATC_y Cuts at: 0 92 578 1200 Size: 92 486 622 Fragments arranged by size: 622 486 92 Bsu36I CC'TnA_GG Cuts at: 0 218 331 883 1200 Size: 218 113 552 317 Fragments arranged by size: 552 317 218 113 BtgI C'CryG_G Cuts at: 0 353 839 1200 Size: 353 486 361 Fragments arranged by size: 486 361 353 DraIII CAC_nnn'GTG Cuts at: 0 519 1200 Size: 519 681 DrdI GACnn_nn'nnGTC Cuts at: 0 382 868 1200 Size: 382 486 332 Fragments arranged by size: 486 382 332 EaeI y'GGCC_r Cuts at: 0 50 756 992 1178 1200 Size: 50 706 236 186 22 Fragments arranged by size: 706 236 186 50 22 EagI C'GGCC_G Cuts at: 0 1178 1200 Size: 1178 22 EarI CTCTTCn'nnn_ Cuts at: 0 672 1200 Size: 672 528 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 76 583 739 1200 Size: 76 507 156 461 Fragments arranged by size: 507 461 156 76 EcoICRI GAG'CTC Cuts at: 0 981 1200 Size: 981 219 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 298 425 727 766 1125 1159 1200 Size: 146 152 127 302 39 359 34 41 Fragments arranged by size: 359 302 152 146 127 41 39 34 EcoO109I rG'GnC_Cy Cuts at: 0 121 153 342 474 1200 Size: 121 32 189 132 726 Fragments arranged by size: 726 189 132 121 32 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 411 1200 Size: 411 789 HaeII r_GCGC'y Cuts at: 0 645 685 795 1146 1200 Size: 645 40 110 351 54 Fragments arranged by size: 645 351 110 54 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 289 321 889 921 1200 Size: 289 32 568 32 279 Fragments arranged by size: 568 289 279 32 32 HincII GTy'rAC Cuts at: 0 849 1200 Size: 849 351 KasI G'GCGC_C Cuts at: 0 791 1200 Size: 791 409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 695 1200 Size: 695 505 MscI TGG'CCA Cuts at: 0 52 994 1200 Size: 52 942 206 Fragments arranged by size: 942 206 52 MslI CAynn'nnrTG Cuts at: 0 58 1106 1200 Size: 58 1048 94 Fragments arranged by size: 1048 94 58 MspA1I CmG'CkG Cuts at: 0 488 654 679 1200 Size: 488 166 25 521 Fragments arranged by size: 521 488 166 25 NarI GG'CG_CC Cuts at: 0 792 1200 Size: 792 408 NcoI C'CATG_G Cuts at: 0 353 839 1200 Size: 353 486 361 Fragments arranged by size: 486 361 353 PasI CC'CwG_GG Cuts at: 0 1068 1200 Size: 1068 132 PfoI T'CCnGG_A Cuts at: 0 38 1200 Size: 38 1162 PpuMI rG'GwC_Cy Cuts at: 0 121 474 1200 Size: 121 353 726 Fragments arranged by size: 726 353 121 PspOMI G'GGCC_C Cuts at: 0 70 1200 Size: 70 1130 PspXI vC'TCGA_Gb Cuts at: 0 637 1200 Size: 637 563 PstI C_TGCA'G Cuts at: 0 195 1121 1200 Size: 195 926 79 Fragments arranged by size: 926 195 79 PvuI CG_AT'CG Cuts at: 0 21 1200 Size: 21 1179 SacI G_AGCT'C Cuts at: 0 983 1200 Size: 983 217 SfcI C'TryA_G Cuts at: 0 191 321 780 1117 1200 Size: 191 130 459 337 83 Fragments arranged by size: 459 337 191 130 83 SfiI GGCCn_nnn'nGGCC Cuts at: 0 78 1200 Size: 78 1122 SfoI GGC'GCC Cuts at: 0 793 1200 Size: 793 407 SmaI CCC'GGG Cuts at: 0 69 112 1200 Size: 69 43 1088 Fragments arranged by size: 1088 69 43 SmlI C'TyrA_G Cuts at: 0 346 601 637 1200 Size: 346 255 36 563 Fragments arranged by size: 563 346 255 36 StyI C'CwwG_G Cuts at: 0 201 267 338 353 839 1200 Size: 201 66 71 15 486 361 Fragments arranged by size: 486 361 201 71 66 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 441 473 1200 Size: 441 32 727 Fragments arranged by size: 727 441 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1002 1200 Size: 1002 198 XhoI C'TCGA_G Cuts at: 0 637 1200 Size: 637 563 XmaI C'CCGG_G Cuts at: 0 67 110 1200 Size: 67 43 1090 Fragments arranged by size: 1090 67 43 ZraI GAC'GTC Cuts at: 0 846 1200 Size: 846 354 Enzymes that do cut: AatII AcuI AfeI AflIII AleI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BstAPI BstXI BstYI Bsu36I BtgI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI PasI PfoI PpuMI PspOMI PspXI PstI PvuI SacI SfcI SfiI SfoI SmaI SmlI StyI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XmnI