Restriction map for
pFN21AE6695
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 949 CGCG CG^CG
AcsI 378 AAATTT [GA]^AATT[CT]
AflIII 959 ACATGT A^C[GA][CT]GT
ApaI 443 GGGCCC GGGCC^C
ApoI 378 AAATTT [GA]^AATT[CT]
BamHI 878 GGATCC G^GATCC
BanII 443 GGGCCC G[GA]GC[CT]^C
BbsI 604 GAAGAC GAAGAC(2/6)
BcnI 29 CCGGG CC^[GC]GG
BlpI 305 GCTCAGC GC^T[ACGT]AGC
BmyI 443 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 305 GCTCAGC GC^T[ACGT]AGC
BpuAI 604 GAAGAC GAAGAC(2/6)
BsaI 550 GGTCTC GGTCTC(1/5)
BsaWI 472 ACCGGA [AT]^CCGG[AT]
BseRI 145 GAGGAG GAGGAG(10/8)
Bsp1286I 443 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 581 TGTACA T^GTACA
BspHI 776 TCATGA T^CATGA
BspLU11I 959 ACATGT A^CATGT
BsrGI 581 TGTACA T^GTACA
BstAPI 844 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 949 CGCG CG^CG
BstYI 878 GGATCC [GA]^GATC[CT]
CelII 305 GCTCAGC GC^T[ACGT]AGC
DraI 223 TTTAAA TTT^AAA
DsaI 356 CCGTGG C^C[GA][CT]GG
MaeII 169 ACGT A^CGT
MflI 878 GGATCC [GA]^GATC[CT]
MseI 224 TTAA T^TAA
MslI 202 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 949 CGCG CG^CG
NciI 29 CCGGG CC^[GC]GG
NspI 959 ACATGT [GA]CATG^[CT]
PflMI 176 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 909 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 163 CTGCAG CTGCA^G
PvuII 502 CAGCTG CAG^CTG
RcaI 776 TCATGA T^CATGA
SspBI 581 TGTACA T^GTACA
Tru9I 224 TTAA T^TAA
Tsp45I 763 GTGAC ^GT[GC]AC
Van91I 176 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 878 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 340 GGTCC G^G[AT]CC
910 GGTCC
BanI 61 GGTGCC G^G[CT][GA]CC
406 GGTGCC
BsmAI 385 GTCTC GTCTC(1/5)
551 GTCTC
BstXI 201 CCACAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
992 CCATCCCTCTGG
EcoT14I 488 CCTTGG C^C[AT][AT]GG
543 CCAAGG
HapII 29 CCGG C^CGG
473 CCGG
HpaII 29 CCGG C^CGG
473 CCGG
MaeIII 320 GTAAC ^GT[ACGT]AC
763 GTGAC
MboII 189 GAAGA GAAGA(8/7)
604 GAAGA
MspA1I 16 CCGCTG C[AC]G^C[GT]G
502 CAGCTG
MspI 29 CCGG C^CGG
473 CCGG
ScaI 805 AGTACT AGT^ACT
846 AGTACT
SfaNI 480 GATGC GCATC(5/9)
794 GCATC
SfcI 135 CTACAG C^T[GA][CT]AG
163 CTGCAG
StyI 488 CCTTGG C^C[AT][AT]GG
543 CCAAGG
XcmI 437 CCACTGGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
621 CCAGGGCAAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MboI MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TaqI TseI
Tsp509I TspRI TthHB8I