Restriction map for
pFN21AE6695
(Circular) (Six-base) MAPSORT of: pFN21AE6695.seq Check: 8469 from: 1 to: 5710
pFN21AE6695 5710 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3773 5157 5425 1632
Size: 2141 1384 268 1917
Fragments arranged by size:
2141 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2964 4609 5586 1203
Size: 1761 1645 977 1327
Fragments arranged by size:
1761 1645 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2193 1184
Size: 1009 4701
AlwNI CAG_nnn'CTG
Cuts at: 2187 2427 2509 3453 5025 2187
Size: 240 82 944 1572 2872
Fragments arranged by size:
2872 1572 944 240 82
ApaI G_GGCC'C
Cuts at: 2452 2452
Size: 5710
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2383 3019 3245 3397 5506 1493
Size: 890 636 226 152 2109 1697
Fragments arranged by size:
2109 1697 890 636 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2267 2300 2267
Size: 33 5677
BamHI G'GATC_C
Cuts at: 2883 3040 3407 2883
Size: 157 367 5186
Fragments arranged by size:
5186 367 157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2411 3031 4486 618
Size: 325 217 627 279 345 620 1455 1842
Fragments arranged by size:
1842 1455 627 620 345 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2452 3029 1391
Size: 1061 577 4072
Fragments arranged by size:
4072 1061 577
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2616 928
Size: 971 717 4022
Fragments arranged by size:
4022 971 717
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4321 136
Size: 107 122 71 631 605 337 2312 1525
Fragments arranged by size:
2312 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 2311 3127 2311
Size: 816 4894
Bme1580I G_kGCm'C
Cuts at: 1790 2452 3771 4927 1790
Size: 662 1319 1156 2573
Fragments arranged by size:
2573 1319 1156 662
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2454 2914 3441
Size: 416 53 83 399 24 1037 460 527
Cuts at: 3441 4399 442
Size: 958 1753
Fragments arranged by size:
1753 1037 958 527 460 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2209 2474 2791 2814 4370 1239
Size: 970 265 317 23 1556 2579
Fragments arranged by size:
2579 1556 970 317 265 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2561 4373 882
Size: 617 1062 1812 2219
Fragments arranged by size:
2219 1812 1062 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2477 4143 4541 4815 4962 5373 2477
Size: 1666 398 274 147 411 2814
Fragments arranged by size:
2814 1666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 1178
Size: 30 165 30 640 30 4815
Fragments arranged by size:
4815 640 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 1186
Size: 979 4731
BseYI C'CCAG_C
Cuts at: 2561 4913 5409 2561
Size: 2352 496 2862
Fragments arranged by size:
2862 2352 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2452 3029 3771 3856 4927 1391
Size: 399 662 577 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 662 577 399 85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 2781 3599 5329 2781
Size: 818 1730 3162
Fragments arranged by size:
3162 1730 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3065 844
Size: 2221 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 2586 96
Size: 2490 3220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2855 794
Size: 2061 3649
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2213 3004 2213
Size: 791 4919
BstYI r'GATC_y
Cuts at: 1972 2883 3040 3407 3792 3809 5261 5347 5359
Size: 911 157 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 911 385 367 346 157 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2361 513
Size: 552 1296 3862
Fragments arranged by size:
3862 1296 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 2732 487
Size: 683 56 1229 277 3465
Fragments arranged by size:
3465 1229 683 277 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 2230 3014 2230
Size: 784 4926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675
Size: 54 1425 50 275 18 1327 889 1629
Cuts at: 5675 8
Size: 43
Fragments arranged by size:
1629 1425 1327 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2386 2513 4297 4671 4817 387
Size: 1064 138 797 127 1784 374 146 1280
Fragments arranged by size:
1784 1280 1064 797 374 146 138 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2209 2474 2791 2814 3773 4370 5157
Size: 393 577 265 317 23 959 597 787
Cuts at: 5157 5425 1239
Size: 268 1524
Fragments arranged by size:
1524 959 787 597 577 393 317 268
265 23
EcoO109I rG'GnC_Cy
Cuts at: 2037 2448 2449 2915 2037
Size: 411 1 466 4832
Fragments arranged by size:
4832 466 411 1
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 4857 1678
Size: 3179 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2075 4351 4383 4425
Size: 32 135 32 471 32 2276 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 2276 471 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2677 4824 5008 1305
Size: 1372 2147 184 2007
Fragments arranged by size:
2147 2007 1372 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 3668 4027 4186 518
Size: 648 1045 1457 359 159 2042
Fragments arranged by size:
2042 1457 1045 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2509 3126 3803 4951 5196 1606
Size: 417 486 617 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 617 486 417 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5158
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2968 3068 4613 1132
Size: 97 1739 100 1545 2229
Fragments arranged by size:
2229 1739 1545 100 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 2964 4609 2964
Size: 1645 4065
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2187 1108
Size: 1079 4631
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2915 2915
Size: 5710
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspOMI G'GGCC_C
Cuts at: 2448 2448
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2172 3062 830
Size: 1342 890 3478
Fragments arranged by size:
3478 1342 890
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2509 2509
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SanDI GG'GwC_CC
Cuts at: 2915 2915
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 2812 2853 3958 5670 2812
Size: 41 1105 1712 2852
Fragments arranged by size:
2852 1712 1105 41
SfcI C'TryA_G
Cuts at: 826 1045 2140 2168 3058 4193 4874 5065 826
Size: 219 1095 28 890 1135 681 191 1471
Fragments arranged by size:
1471 1135 1095 890 681 219 191 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2493 2548 3149 513
Size: 552 1428 55 601 3074
Fragments arranged by size:
3074 1428 601 552 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 3737 3754 3907 4092 4537 325
Size: 1255 1014 1143 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1143 1014 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 2810 2851
Size: 274 80 33 51 1443 609 224 41
Cuts at: 2851 3956 5668 96
Size: 1105 1712 138
Fragments arranged by size:
1712 1443 1105 609 274 224 138 80
51 41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2590 2622 5670 5702 2590
Size: 32 3048 32 2598
Fragments arranged by size:
3048 2598 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 2633 3004 2449
Size: 184 371 5155
Fragments arranged by size:
5155 371 184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI