Restriction map for
pFN21AE6694
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AgeI AlwNI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpmI BsaBI
BsaI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MflI MluI
MluNI MscI MseI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 1072 TCCGGA T^CCGGA
AflIII 1115 ACGTGT A^C[GA][CT]GT
AhdI 502 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 315 GTGCAC G^TGCAC
AlwI 528 GGATC GGATC(4/5)
Aor51HI 540 AGCGCT AGC^GCT
ApaLI 315 GTGCAC G^TGCAC
AspEI 502 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 208 GTCTTC GAAGAC(2/6)
BfaI 574 CTAG C^TAG
BpuAI 208 GTCTTC GAAGAC(2/6)
BsaWI 1072 TCCGGA [AT]^CCGG[AT]
BseAI 1072 TCCGGA T^CCGGA
BsmAI 1010 GAGAC GTCTC(1/5)
BspEI 1072 TCCGGA T^CCGGA
BsrDI 653 CATTGC GCAATG(2/0)
BsrI 324 CCAGT ACTGG(1/-1)
BssHII 442 GCGCGC G^CGCGC
BstF5I 498 GGATG GGATG(2/0)
BstXI 1136 CCAGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 529 GATC ^GATC
Eam1105I 502 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 448 CTCTTC CTCTTC(1/4)
Eco47III 540 AGCGCT AGC^GCT
FokI 498 GGATG GGATG(9/13)
FseI 738 GGCCGGCC GGCCGG^CC
HinfI 1012 GACTC G^A[ACGT]TC
Ksp632I 448 CTCTTC CTCTTC(1/4)
MaeI 574 CTAG C^TAG
MaeIII 1102 GTGAC ^GT[ACGT]AC
MboI 529 GATC ^GATC
MroI 1072 TCCGGA T^CCGGA
MslI 612 CATGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 611 CCATGG C^CATGG
NdeII 529 GATC ^GATC
PleI 1012 GACTC GAGTC(4/5)
Sau3AI 529 GATC ^GATC
SfaNI 152 GCATC GCATC(5/9)
Tsp45I 1102 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 315 GTGCAC G[AT]GC[AT]^C
724 GTGCTC
AvaII 277 GGTCC G^G[AT]CC
1140 GGTCC
BbrPI 250 CACGTG CAC^GTG
1114 CACGTG
BsaAI 250 CACGTG [CT]AC^GT[GA]
1114 CACGTG
BsgI 247 CTGCAC GTGCAG(16/14)
253 GTGCAG
BsiHKAI 315 GTGCAC G[AT]GC[AT]^C
724 GTGCTC
BspMI 239 ACCTGC ACCTGC(4/8)
351 ACCTGC
BsrBI 844 CCGCTC CCGCTC(-3/-3)
295 GAGCGG
EcoT14I 611 CCATGG C^C[AT][AT]GG
641 CCAAGG
PaeR7I 337 CTCGAG C^TCGAG
483 CTCGAG
PmaCI 250 CACGTG CAC^GTG
1114 CACGTG
PmlI 250 CACGTG CAC^GTG
1114 CACGTG
PpuMI 276 GGGTCCC [GA]G^G[AT]CC[CT]
1139 GGGTCCT
PstI 99 CTGCAG CTGCA^G
376 CTGCAG
SfcI 99 CTGCAG C^T[GA][CT]AG
376 CTGCAG
SfiI 261 GGCCCCAACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
423 GGCCTTCTGGGCC
SmlI 337 CTCGAG C^T[CT][GA]AG
483 CTCGAG
StyI 611 CCATGG C^C[AT][AT]GG
641 CCAAGG
TaqI 338 TCGA T^CGA
484 TCGA
TthHB8I 338 TCGA T^CGA
484 TCGA
XhoI 337 CTCGAG C^TCGAG
483 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI BanI BanII BbeI BbvI
BcnI BglI BlpI BmyI Bpu1102I
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
KspI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIII NlaIV
SacII Sau96I ScrFI SfoI SmaI
TseI TspRI XmaCI XmaI