(Circular) (Six-base) MAPSORT of: pFN21AE6694.seq Check: 711 from: 1 to: 5899 pFN21AE6694 5899 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2365 2365 Size: 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3962 5346 5614 1632 Size: 2330 1384 268 1917 Fragments arranged by size: 2330 1917 1384 268 AfeI AGC'GCT Cuts at: 2547 2547 Size: 5899 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 3120 4798 5775 1203 Size: 1917 1678 977 1327 Fragments arranged by size: 1917 1678 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 2512 4628 2512 Size: 2116 3783 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 3642 5214 3642 Size: 1572 4327 ApaI G_GGCC'C Cuts at: 2096 2269 2440 3027 2096 Size: 173 171 587 4968 Fragments arranged by size: 4968 587 173 171 ApaLI G'TGCA_C Cuts at: 2320 3956 5112 2320 Size: 1636 1156 3107 Fragments arranged by size: 3107 1636 1156 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2084 2131 2285 2342 2488 2633 Size: 73 127 14 47 154 57 146 145 Cuts at: 2633 3129 3190 3224 1870 Size: 496 61 34 4545 Fragments arranged by size: 4545 496 154 146 145 127 73 61 57 47 34 14 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2684 2783 2845 3062 Size: 325 217 627 420 477 99 62 217 Cuts at: 3062 3091 3220 4675 618 Size: 29 129 1455 1842 Fragments arranged by size: 1842 1455 627 477 420 325 217 217 129 99 62 29 BanII G_rGCy'C Cuts at: 1391 2096 2269 2349 2440 3027 3077 3218 1391 Size: 705 173 80 91 587 50 141 4072 Fragments arranged by size: 4072 705 587 173 141 91 80 50 BbeI G_GCGC'C Cuts at: 2211 2688 2849 2211 Size: 477 161 5261 Fragments arranged by size: 5261 477 161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2206 928 Size: 971 307 4621 Fragments arranged by size: 4621 971 307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 2045 2072 Size: 107 122 71 631 605 361 12 27 Cuts at: 2072 2154 2273 2435 2781 4510 136 Size: 82 119 162 346 1729 1525 Fragments arranged by size: 1729 1525 631 605 361 346 162 122 119 107 82 71 27 12 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 2400 2417 2717 2732 3316 2400 Size: 17 300 15 584 4983 Fragments arranged by size: 4983 584 300 17 15 Bme1580I G_kGCm'C Cuts at: 1790 2096 2269 2324 2440 2646 3027 3960 5116 Size: 306 173 55 116 206 381 933 1156 Cuts at: 5116 1790 Size: 2573 Fragments arranged by size: 2573 1156 933 381 306 206 173 116 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5899 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 3630 4588 Size: 416 53 83 399 24 906 1307 958 Cuts at: 4588 442 Size: 1753 Fragments arranged by size: 1753 1307 958 906 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4559 1239 Size: 3320 2579 Bpu10I CC'TnA_GC Cuts at: 2120 2120 Size: 5899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4024 4889 5187 4024 Size: 865 298 4736 Fragments arranged by size: 4736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2257 3121 3690 493 Size: 711 1053 864 569 2702 Fragments arranged by size: 2702 1053 864 711 569 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 2374 2685 2846 Size: 53 83 186 936 675 166 311 161 Cuts at: 2846 2991 3706 4088 4723 275 Size: 145 715 382 635 1451 Fragments arranged by size: 1451 936 715 675 635 382 311 186 166 161 145 83 53 BsaWI w'CCGG_w Cuts at: 3077 4332 4730 5004 5151 5562 3077 Size: 1255 398 274 147 411 3414 Fragments arranged by size: 3414 1255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3072 3102 1178 Size: 30 165 30 1669 30 3975 Fragments arranged by size: 3975 1669 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2540 2603 2612 2615 1186 Size: 1354 63 9 3 4470 Fragments arranged by size: 4470 1354 63 9 3 BseYI C'CCAG_C Cuts at: 2548 2566 5102 5598 2548 Size: 18 2536 496 2849 Fragments arranged by size: 2849 2536 496 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2279 1163 Size: 573 501 42 4783 Fragments arranged by size: 4783 573 501 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2205 2277 3349 4110 5138 Size: 275 186 39 165 72 1072 761 1028 Cuts at: 5138 1540 Size: 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 165 72 39 BsiHKAI G_wGCw'C Cuts at: 2324 2733 3960 4045 5116 2324 Size: 409 1227 85 1071 3107 Fragments arranged by size: 3107 1227 1071 409 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2269 2324 2349 2440 2646 2733 Size: 399 306 173 55 25 91 206 87 Cuts at: 2733 3027 3077 3218 3960 4045 5116 1391 Size: 294 50 141 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 294 206 173 141 91 87 85 55 50 25 BspEI T'CCGG_A Cuts at: 3077 5562 3077 Size: 2485 3414 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2253 2365 3254 844 Size: 1409 112 889 3489 Fragments arranged by size: 3489 1409 889 112 BsrBI CCG'CTC Cuts at: 1394 1823 2302 2851 3786 1394 Size: 429 479 549 935 3507 Fragments arranged by size: 3507 935 549 479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2657 4394 4568 65 Size: 1183 1409 1737 174 1396 Fragments arranged by size: 1737 1409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2204 2744 2864 4543 4730 1700 Size: 110 394 540 120 1679 187 2869 Fragments arranged by size: 2869 1679 540 394 187 120 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 2447 1925 Size: 522 5377 BssSI C'ACGA_G Cuts at: 3959 4971 3959 Size: 1012 4887 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstXI CCAn_nnnn'nTGG Cuts at: 3148 3148 Size: 5899 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2096 2149 2199 2277 2368 2616 2817 Size: 552 1031 53 50 78 91 248 201 Cuts at: 2817 2996 3039 513 Size: 179 43 3373 Fragments arranged by size: 3373 1031 552 248 201 179 91 78 53 50 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 2021 2267 2750 3051 3415 4209 4229 2021 Size: 246 483 301 364 794 20 3691 Fragments arranged by size: 3691 794 483 364 301 246 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2202 2274 Size: 54 1425 50 275 18 207 165 72 Cuts at: 2274 2742 2964 3346 4235 5864 8 Size: 468 222 382 889 1629 43 Fragments arranged by size: 1629 1425 889 468 382 275 222 207 165 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2037 2202 2274 3346 1537 Size: 275 225 165 72 1072 4090 Fragments arranged by size: 4090 1072 275 225 165 72 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 3829 1353 Size: 1106 1370 3423 Fragments arranged by size: 3423 1370 1106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2546 4486 4860 5006 387 Size: 1064 138 957 1940 374 146 1280 Fragments arranged by size: 1940 1280 1064 957 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3962 4559 5346 5614 1239 Size: 393 2330 597 787 268 1524 Fragments arranged by size: 2330 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2266 2282 3024 3145 2266 Size: 16 742 121 5020 Fragments arranged by size: 5020 742 121 16 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FseI GG_CCGG'CC Cuts at: 2748 2748 Size: 5899 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 2067 2211 2485 2549 2688 2828 2837 2849 Size: 389 144 274 64 139 140 9 12 Cuts at: 2849 5046 1678 Size: 2197 2531 Fragments arranged by size: 2531 2197 389 274 144 140 139 64 12 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 4540 4572 4614 Size: 32 135 32 926 32 2010 32 42 Cuts at: 4614 4646 1373 Size: 32 2626 Fragments arranged by size: 2626 2010 926 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KasI G'GCGC_C Cuts at: 2207 2684 2845 2207 Size: 477 161 5261 Fragments arranged by size: 5261 477 161 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 2837 5013 5197 1305 Size: 1191 341 2176 184 2007 Fragments arranged by size: 2176 2007 1191 341 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 3857 4216 4375 518 Size: 648 1455 1236 359 159 2042 Fragments arranged by size: 2042 1455 1236 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2109 2201 2279 2370 2471 2819 2957 3041 Size: 503 92 78 91 101 348 138 84 Cuts at: 3041 3315 3992 5140 5385 1606 Size: 274 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 503 348 274 245 138 101 92 91 84 78 NaeI GCC'GGC Cuts at: 2206 2746 2866 2206 Size: 540 120 5239 Fragments arranged by size: 5239 540 120 NarI GG'CG_CC Cuts at: 2208 2685 2846 2208 Size: 477 161 5261 Fragments arranged by size: 5261 477 161 NcoI C'CATG_G Cuts at: 513 1065 2616 513 Size: 552 1551 3796 Fragments arranged by size: 3796 1551 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NgoMIV G'CCGG_C Cuts at: 2204 2744 2864 2204 Size: 540 120 5239 Fragments arranged by size: 5239 540 120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 2312 1348 Size: 964 4935 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5899 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PmlI CAC'GTG Cuts at: 2257 3121 2257 Size: 864 5035 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2282 3145 2282 Size: 863 5036 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2092 2265 2436 3023 2092 Size: 173 171 587 4968 Fragments arranged by size: 4968 587 173 171 PspXI vC'TCGA_Gb Cuts at: 2342 2342 Size: 5899 PstI C_TGCA'G Cuts at: 830 2108 2385 3251 830 Size: 1278 277 866 3478 Fragments arranged by size: 3478 1278 866 277 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 SacII CC_GC'GG Cuts at: 2202 2280 2371 2820 3042 2202 Size: 78 91 449 222 5059 Fragments arranged by size: 5059 449 222 91 78 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SanDI GG'GwC_CC Cuts at: 2282 2282 Size: 5899 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2104 2381 3247 4382 5063 5254 826 Size: 219 1059 277 866 1135 681 191 1471 Fragments arranged by size: 1471 1135 1059 866 681 277 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2273 2435 2273 Size: 162 5737 SfoI GGC'GCC Cuts at: 2209 2686 2847 2209 Size: 477 161 5261 Fragments arranged by size: 5261 477 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 2072 2133 2287 3192 3226 1872 Size: 200 61 154 905 34 4545 Fragments arranged by size: 4545 905 200 154 61 34 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 2488 4003 4904 5166 820 Size: 197 926 399 146 1515 901 262 1553 Fragments arranged by size: 1553 1515 926 901 399 262 197 146 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SrfI GCCC'GGGC Cuts at: 2133 2133 Size: 5899 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 2616 2646 3338 513 Size: 552 1551 30 692 3074 Fragments arranged by size: 3074 1551 692 552 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2863 3926 3943 4096 4281 4726 325 Size: 1255 1283 1063 17 153 185 445 1498 Fragments arranged by size: 1498 1283 1255 1063 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3072 3104 5859 5891 3072 Size: 32 2755 32 3080 Fragments arranged by size: 3080 2755 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 2342 2488 1943 Size: 399 146 5354 Fragments arranged by size: 5354 399 146 XmaI C'CCGG_G Cuts at: 1870 2070 2131 2285 3190 3224 1870 Size: 200 61 154 905 34 4545 Fragments arranged by size: 4545 905 200 154 61 34 XmnI GAAnn'nnTTC Cuts at: 4028 4028 Size: 5899 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PsrI PvuII RsrII SacI SapI SexAI SwaI XcmI