(Linear) (Six-base) MAPSORT of: KIEE6694.seq Check: 8320 from: 1 to: 1194 KIEE6694.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 360 1194 Size: 360 834 AfeI AGC'GCT Cuts at: 0 542 1194 Size: 542 652 AflIII A'CryG_T Cuts at: 0 1115 1194 Size: 1115 79 AhdI GACnn_n'nnGTC Cuts at: 0 507 1194 Size: 507 687 ApaI G_GGCC'C Cuts at: 0 91 264 435 1022 1194 Size: 91 173 171 587 172 Fragments arranged by size: 587 173 172 171 91 ApaLI G'TGCA_C Cuts at: 0 315 1194 Size: 315 879 AvaI C'yCGr_G Cuts at: 0 65 79 126 280 337 483 628 1124 Size: 65 14 47 154 57 146 145 496 Cuts at: 1124 1185 1194 Size: 61 9 Fragments arranged by size: 496 154 146 145 65 61 57 47 14 9 BanI G'GyrC_C Cuts at: 0 202 679 778 840 1057 1086 1194 Size: 202 477 99 62 217 29 108 Fragments arranged by size: 477 217 202 108 99 62 29 BanII G_rGCy'C Cuts at: 0 91 264 344 435 1022 1072 1194 Size: 91 173 80 91 587 50 122 Fragments arranged by size: 587 173 122 91 91 80 50 BbeI G_GCGC'C Cuts at: 0 206 683 844 1194 Size: 206 477 161 350 Fragments arranged by size: 477 350 206 161 BbsI GAAGACnn'nnnn_ Cuts at: 0 201 1194 Size: 201 993 BglI GCCn_nnn'nGGC Cuts at: 0 28 40 67 149 268 430 776 1194 Size: 28 12 27 82 119 162 346 418 Fragments arranged by size: 418 346 162 119 82 28 27 12 BlpI GC'TnA_GC Cuts at: 0 395 412 712 727 1194 Size: 395 17 300 15 467 Fragments arranged by size: 467 395 300 17 15 Bme1580I G_kGCm'C Cuts at: 0 91 264 319 435 641 1022 1194 Size: 91 173 55 116 206 381 172 Fragments arranged by size: 381 206 173 172 116 91 55 BmrI ACTGGGnnnn_n' Cuts at: 0 318 1194 Size: 318 876 Bpu10I CC'TnA_GC Cuts at: 0 115 1194 Size: 115 1079 BsaAI yAC'GTr Cuts at: 0 252 1116 1194 Size: 252 864 78 Fragments arranged by size: 864 252 78 BsaHI Gr'CG_yC Cuts at: 0 203 369 680 841 986 1194 Size: 203 166 311 161 145 208 Fragments arranged by size: 311 208 203 166 161 145 BsaWI w'CCGG_w Cuts at: 0 1072 1194 Size: 1072 122 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1067 1097 1194 Size: 1067 30 97 Fragments arranged by size: 1067 97 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 535 598 607 610 1194 Size: 535 63 9 3 584 Fragments arranged by size: 584 535 63 9 3 BseYI C'CCAG_C Cuts at: 0 543 561 1194 Size: 543 18 633 Fragments arranged by size: 633 543 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 274 1194 Size: 232 42 920 Fragments arranged by size: 920 232 42 BsiEI CG_ry'CG Cuts at: 0 35 200 272 1194 Size: 35 165 72 922 Fragments arranged by size: 922 165 72 35 BsiHKAI G_wGCw'C Cuts at: 0 319 728 1194 Size: 319 409 466 Fragments arranged by size: 466 409 319 Bsp1286I G_dGCh'C Cuts at: 0 91 264 319 344 435 641 728 1022 Size: 91 173 55 25 91 206 87 294 Cuts at: 1022 1072 1194 Size: 50 122 Fragments arranged by size: 294 206 173 122 91 91 87 55 50 25 BspEI T'CCGG_A Cuts at: 0 1072 1194 Size: 1072 122 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 248 360 1194 Size: 248 112 834 Fragments arranged by size: 834 248 112 BsrBI CCG'CTC Cuts at: 0 297 846 1194 Size: 297 549 348 Fragments arranged by size: 549 348 297 BsrDI GCAATG_nn' Cuts at: 0 652 1194 Size: 652 542 BsrFI r'CCGG_y Cuts at: 0 199 739 859 1194 Size: 199 540 120 335 Fragments arranged by size: 540 335 199 120 BssHII G'CGCG_C Cuts at: 0 442 1194 Size: 442 752 BstXI CCAn_nnnn'nTGG Cuts at: 0 1143 1194 Size: 1143 51 BtgI C'CryG_G Cuts at: 0 91 144 194 272 363 611 812 991 Size: 91 53 50 78 91 248 201 179 Cuts at: 991 1034 1194 Size: 43 160 Fragments arranged by size: 248 201 179 160 91 91 78 53 50 43 BtsI GCAGTG_nn' Cuts at: 0 16 262 745 1046 1194 Size: 16 246 483 301 148 Fragments arranged by size: 483 301 246 148 16 EaeI y'GGCC_r Cuts at: 0 32 197 269 737 959 1194 Size: 32 165 72 468 222 235 Fragments arranged by size: 468 235 222 165 72 32 EagI C'GGCC_G Cuts at: 0 32 197 269 1194 Size: 32 165 72 925 Fragments arranged by size: 925 165 72 32 EarI CTCTTCn'nnn_ Cuts at: 0 454 1194 Size: 454 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 541 1194 Size: 541 653 EcoO109I rG'GnC_Cy Cuts at: 0 261 277 1019 1140 1194 Size: 261 16 742 121 54 Fragments arranged by size: 742 261 121 54 16 FseI GG_CCGG'CC Cuts at: 0 743 1194 Size: 743 451 HaeII r_GCGC'y Cuts at: 0 62 206 480 544 683 823 832 844 Size: 62 144 274 64 139 140 9 12 Cuts at: 844 1194 Size: 350 Fragments arranged by size: 350 274 144 140 139 64 62 12 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 493 525 1194 Size: 493 32 669 Fragments arranged by size: 669 493 32 KasI G'GCGC_C Cuts at: 0 202 679 840 1194 Size: 202 477 161 354 Fragments arranged by size: 477 354 202 161 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 491 832 1194 Size: 491 341 362 Fragments arranged by size: 491 362 341 MslI CAynn'nnrTG Cuts at: 0 616 1194 Size: 616 578 MspA1I CmG'CkG Cuts at: 0 104 196 274 365 466 814 952 1036 Size: 104 92 78 91 101 348 138 84 Cuts at: 1036 1194 Size: 158 Fragments arranged by size: 348 158 138 104 101 92 91 84 78 NaeI GCC'GGC Cuts at: 0 201 741 861 1194 Size: 201 540 120 333 Fragments arranged by size: 540 333 201 120 NarI GG'CG_CC Cuts at: 0 203 680 841 1194 Size: 203 477 161 353 Fragments arranged by size: 477 353 203 161 NcoI C'CATG_G Cuts at: 0 611 1194 Size: 611 583 NgoMIV G'CCGG_C Cuts at: 0 199 739 859 1194 Size: 199 540 120 335 Fragments arranged by size: 540 335 199 120 PasI CC'CwG_GG Cuts at: 0 307 1194 Size: 307 887 PmlI CAC'GTG Cuts at: 0 252 1116 1194 Size: 252 864 78 Fragments arranged by size: 864 252 78 PpuMI rG'GwC_Cy Cuts at: 0 277 1140 1194 Size: 277 863 54 Fragments arranged by size: 863 277 54 PspOMI G'GGCC_C Cuts at: 0 87 260 431 1018 1194 Size: 87 173 171 587 176 Fragments arranged by size: 587 176 173 171 87 PspXI vC'TCGA_Gb Cuts at: 0 337 1194 Size: 337 857 PstI C_TGCA'G Cuts at: 0 103 380 1194 Size: 103 277 814 Fragments arranged by size: 814 277 103 SacII CC_GC'GG Cuts at: 0 197 275 366 815 1037 1194 Size: 197 78 91 449 222 157 Fragments arranged by size: 449 222 197 157 91 78 SanDI GG'GwC_CC Cuts at: 0 277 1194 Size: 277 917 SfcI C'TryA_G Cuts at: 0 99 376 1194 Size: 99 277 818 Fragments arranged by size: 818 277 99 SfiI GGCCn_nnn'nGGCC Cuts at: 0 268 430 1194 Size: 268 162 764 Fragments arranged by size: 764 268 162 SfoI GGC'GCC Cuts at: 0 204 681 842 1194 Size: 204 477 161 352 Fragments arranged by size: 477 352 204 161 SmaI CCC'GGG Cuts at: 0 67 128 282 1187 1194 Size: 67 61 154 905 7 Fragments arranged by size: 905 154 67 61 7 SmlI C'TyrA_G Cuts at: 0 337 483 1194 Size: 337 146 711 Fragments arranged by size: 711 337 146 SrfI GCCC'GGGC Cuts at: 0 128 1194 Size: 128 1066 StyI C'CwwG_G Cuts at: 0 611 641 1194 Size: 611 30 553 Fragments arranged by size: 611 553 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 858 1194 Size: 858 336 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1067 1099 1194 Size: 1067 32 95 Fragments arranged by size: 1067 95 32 XhoI C'TCGA_G Cuts at: 0 337 483 1194 Size: 337 146 711 Fragments arranged by size: 711 337 146 XmaI C'CCGG_G Cuts at: 0 65 126 280 1185 1194 Size: 65 61 154 905 9 Fragments arranged by size: 905 154 65 61 9 Enzymes that do cut: AarI AfeI AflIII AhdI ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmrI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstXI BtgI BtsI EaeI EagI EarI EciI EcoO109I FseI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PmlI PpuMI PspOMI PspXI PstI SacII SanDI SfcI SfiI SfoI SmaI SmlI SrfI StyI TaqII TstI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AgeI AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspHI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TatI Tth111I XbaI XcmI XmnI ZraI