################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvrII BalI BanI BbeI BbrPI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflII 390 CTTAAG C^TTAAG AflIII 213 ACATGT A^C[GA][CT]GT Asp700I 4 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 600 GGACC G^G[AT]CC AviII 296 TGCGCA TGC^GCA BamHI 42 GGATCC G^GATCC BanII 531 GGGCTC G[GA]GC[CT]^C BbsI 424 GAAGAC GAAGAC(2/6) BbvCI 763 CCTCAGC CCTCAGC(-5/-2) BclI 372 TGATCA T^GATCA BfaI 793 CTAG C^TAG BfrI 390 CTTAAG C^TTAAG BmyI 531 GGGCTC G[AGT]GC[ACT]^C BpmI 536 CTCCAG CTGGAG(16/14) BpuAI 424 GAAGAC GAAGAC(2/6) BsaBI 373 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 244 GTCTC GTCTC(1/5) Bsp1286I 531 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 411 TGTACA T^GTACA BspLU11I 213 ACATGT A^CATGT BspMI 183 ACCTGC ACCTGC(4/8) BsrGI 411 TGTACA T^GTACA Cac8I 565 GCCAGC GC[ACGT]^[ACGT]GC CfoI 297 GCGC GCG^C Cfr13I 600 GGACC G^G[ACGT]CC EcoT14I 480 CCAAGG C^C[AT][AT]GG FbaI 372 TGATCA T^GATCA FspI 296 TGCGCA TGC^GCA HaeIII 252 GGCC GG^CC HhaI 297 GCGC GCG^C HinP1I 297 GCGC G^CGC MaeI 793 CTAG C^TAG MaeII 678 ACGT A^CGT MamI 373 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 65 CAGCTG C[AC]G^C[GT]G NlaIV 42 GGATCC GG[ACGT]^[ACGT]CC NspI 213 ACATGT [GA]CATG^[CT] PshAI 572 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 65 CAGCTG CAG^CTG SapI 187 GCTCTTC GCTCTTC(1/4) Sau96I 600 GGACC G^G[ACGT]CC SexAI 684 ACCTGGT A^CC[AT]GGT SfaNI 732 GATGC GCATC(5/9) SspBI 411 TGTACA T^GTACA StuI 251 AGGCCT AGG^CCT StyI 480 CCAAGG C^C[AT][AT]GG Tsp45I 842 GTGAC ^GT[GC]AC XmnI 4 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 457 AAATTT [GA]^AATT[CT] 485 GAATTT AlwNI 242 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 353 CAGAATCTG ApoI 457 AAATTT [GA]^AATT[CT] 485 GAATTT BsgI 818 CTGCAC GTGCAG(16/14) 92 GTGCAG BsiYI 591 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 612 CCGAAACCGGG BslI 591 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 612 CCGAAACCGGG BstYI 42 GGATCC [GA]^GATC[CT] 378 GGATCT DdeI 248 CTGAG C^T[ACGT]AG 764 CTCAG EarI 188 CTCTTC CTCTTC(1/4) 282 GAAGAG HindIII 158 AAGCTT A^AGCTT 871 AAGCTT HphI 103 GGTGA GGTGA(8/7) 688 GGTGA Ksp632I 188 CTCTTC CTCTTC(1/4) 282 GAAGAG MaeIII 325 GTAAC ^GT[ACGT]AC 842 GTGAC MflI 42 GGATCC [GA]^GATC[CT] 378 GGATCT MslI 85 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 769 CATATATATG NlaIII 214 CATG CATG^ 472 CATG SmlI 390 CTTAAG C^T[CT][GA]AG 491 CTCAAG TaqI 337 TCGA T^CGA 741 TCGA TspRI 275 CAGTG CA[GC]TG[ACGT][ACGT]^ 345 CAGTG TthHB8I 337 TCGA T^CGA 741 TCGA XhoII 42 GGATCC [GA]^GATC[CT] 378 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI AlwI BbvI BcnI BsaJI BsmFI BsrI BssKI BstNI DpnII EcoRII Fnu4HI HapII HinfI HpaII ItaI MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII PleI RsaI Sau3AI ScrFI SfcI TfiI Tru9I TseI Tsp509I