##################################
# NO CUT ENZYMES (  233 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TaqI        
TfiI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   19 enzymes) #
###################################

AvaII         100  GGACC                G^G[AT]CC
BbvI            6  GCTGC                GCAGC(8/12)
BciVI         124  GTATCC               GTATCC(6/5)
BsiYI          79  CCGTCCATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           79  CCGTCCATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          99  GGGAC                GGGAC(10/14)
BsrDI         132  CATTGC               GCAATG(2/0)
Cac8I          51  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        100  GGACC                G^G[ACGT]CC
Fnu4HI          6  GCTGC                GC^[ACGT]GC
HphI           90  GGTGA                GGTGA(8/7)
ItaI            6  GCTGC                GC^[ACGT]GC
MseI          155  TTAA                 T^TAA
MslI           84  CATAGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII         36  CATG                 CATG^
Sau96I        100  GGACC                G^G[ACGT]CC
SspI          147  AATATT               AAT^ATT
Tru9I         155  TTAA                 T^TAA
TseI            6  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

AluI           66  AGCT                 AG^CT
              182  AGCT                
BsrI           29  ACTGG                ACTGG(1/-1)
               72  ACTGG               
MnlI            3  GAGG                 CCTC(7/6)
               21  GAGG                
NlaIV          75  GGAGCC               GG[ACGT]^[ACGT]CC
               99  GGGACC              
TspRI         104  CACTG                CA[GC]TG[ACGT][ACGT]^
              159  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

BpmI