Restriction map for
pFN21AE6572
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI HgaI
Hin1I HincII HindII HinfI HpaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AluI 226 AGCT AG^CT
AlwI 327 GGATC GGATC(4/5)
ApaI 129 GGGCCC GGGCC^C
AvaI 5 CCCGAG C^[CT]CG[GA]G
AviII 260 TGCGCA TGC^GCA
BanI 307 GGTGCC G^G[CT][GA]CC
BbsI 352 GAAGAC GAAGAC(2/6)
BpuAI 352 GAAGAC GAAGAC(2/6)
BsiEI 315 CGACCG CG[GA][CT]^CG
BsoBI 5 CCCGAG C^[CT]CG[GA]G
BspMI 122 ACCTGC ACCTGC(4/8)
BsrBI 361 GAGCGG CCGCTC(-3/-3)
BstYI 327 GGATCT [GA]^GATC[CT]
DdeI 348 CTCAG C^T[ACGT]AG
DpnII 328 GATC ^GATC
DsaI 44 CCGCGG C^C[GA][CT]GG
EaeI 80 CGGCCA [CT]^GGCC[GA]
EcoT14I 83 CCAAGG C^C[AT][AT]GG
FspI 260 TGCGCA TGC^GCA
HaeII 13 AGCGCC [GA]GCGC^[CT]
HindIII 225 AAGCTT A^AGCTT
HphI 179 TCACC GGTGA(8/7)
KspI 44 CCGCGG CCGC^GG
MboI 328 GATC ^GATC
MflI 327 GGATCT [GA]^GATC[CT]
MspA1I 44 CCGCGG C[AC]G^C[GT]G
NaeI 78 GCCGGC GCC^GGC
NdeII 328 GATC ^GATC
NgoMIV 78 GCCGGC G^CCGGC
NspI 135 ACATGC [GA]CATG^[CT]
SacII 44 CCGCGG CCGC^GG
Sau3AI 328 GATC ^GATC
SfaNI 291 GCATC GCATC(5/9)
StyI 83 CCAAGG C^C[AT][AT]GG
XhoII 327 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 45 CGCG CG^CG
114 CGCG
BanII 67 GGGCTC G[GA]GC[CT]^C
129 GGGCCC
BbvI 11 GCAGC GCAGC(8/12)
340 GCAGC
BsrFI 78 GCCGGC [GA]^CCGG[CT]
304 GCCGGT
BssKI 220 CCAGG ^CC[ACGT]GG
324 CCTGG
BstNI 220 CCAGG CC^[AT]GG
324 CCTGG
BstUI 45 CGCG CG^CG
114 CGCG
Cfr10I 78 GCCGGC [GA]^CCGG[CT]
304 GCCGGT
EcoRII 220 CCAGG ^CC[AT]GG
324 CCTGG
HapII 79 CCGG C^CGG
305 CCGG
HpaII 79 CCGG C^CGG
305 CCGG
MboII 205 GAAGA GAAGA(8/7)
352 GAAGA
MspI 79 CCGG C^CGG
305 CCGG
MvaI 220 CCAGG CC^[AT]GG
324 CCTGG
MvnI 45 CGCG CG^CG
114 CGCG
ScrFI 220 CCAGG CC^[ACGT]GG
324 CCTGG
TaqI 314 TCGA T^CGA
359 TCGA
TseI 11 GCAGC G^C[AT]GC
340 GCAGC
TthHB8I 314 TCGA T^CGA
359 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AvaII BmyI BsaJI BseRI
BsiYI BslI Bsp1286I Cac8I CfoI
Cfr13I FauI Fnu4HI HaeIII HhaI
HinP1I ItaI MnlI MwoI NlaIII
NlaIV Sau96I