(Circular) (Six-base) MAPSORT of: pFN21AE6572.seq Check: 2408 from: 1 to: 5074 pFN21AE6572 5074 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2884 278 Size: 53 83 186 936 1348 2468 Fragments arranged by size: 2468 1348 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2417 1937 Size: 480 4594 Acc65I G'GTAC_C Cuts at: 2395 2395 Size: 5074 AclI AA'CG_TT Cuts at: 1563 3201 3574 1563 Size: 1638 373 3063 Fragments arranged by size: 3063 1638 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3137 4521 4789 1632 Size: 1505 1384 268 1917 Fragments arranged by size: 1917 1505 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4877 AflIII A'CryG_T Cuts at: 1203 3973 4950 1203 Size: 2770 977 1327 Fragments arranged by size: 2770 1327 977 AgeI A'CCGG_T Cuts at: 3905 3905 Size: 5074 AhdI GACnn_n'nnGTC Cuts at: 3803 3803 Size: 5074 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5074 AlwNI CAG_nnn'CTG Cuts at: 2817 4389 2817 Size: 1572 3502 ApaI G_GGCC'C Cuts at: 2138 2138 Size: 5074 ApaLI G'TGCA_C Cuts at: 3131 4287 3131 Size: 1156 3918 ApoI r'AATT_y Cuts at: 1493 2383 2609 2761 4870 1493 Size: 890 226 152 2109 1697 Fragments arranged by size: 2109 1697 890 226 152 AseI AT'TA_AT Cuts at: 160 1032 3628 160 Size: 872 2596 1606 Fragments arranged by size: 2596 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5074 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2399 1870 Size: 73 67 389 4545 Fragments arranged by size: 4545 389 73 67 BamHI G'GATC_C Cuts at: 2404 2771 2404 Size: 367 4707 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 2395 3850 618 Size: 325 217 627 525 83 1455 1842 Fragments arranged by size: 1842 1455 627 525 325 217 83 BanII G_rGCy'C Cuts at: 1391 2076 2138 2393 1391 Size: 685 62 255 4072 Fragments arranged by size: 4072 685 255 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 928 Size: 971 465 3638 Fragments arranged by size: 3638 971 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3265 3299 1903 Size: 34 1328 34 3678 Fragments arranged by size: 3678 1328 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2966 4176 2966 Size: 1210 3864 BclI T'GATC_A Cuts at: 1548 1548 Size: 5074 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3685 136 Size: 107 122 71 631 605 2013 1525 Fragments arranged by size: 2013 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5069 5069 Size: 5074 BlpI GC'TnA_GC Cuts at: 2491 2491 Size: 5074 Bme1580I G_kGCm'C Cuts at: 1790 2138 2317 3135 4291 1790 Size: 348 179 818 1156 2573 Fragments arranged by size: 2573 1156 818 348 179 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2805 3763 442 Size: 416 53 83 399 24 1388 958 1753 Fragments arranged by size: 1753 1388 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5074 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3734 1239 Size: 2495 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3199 4064 4362 3199 Size: 865 298 3911 Fragments arranged by size: 3911 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3737 882 Size: 617 2238 2219 Fragments arranged by size: 2238 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2865 493 Size: 711 1661 2702 Fragments arranged by size: 2702 1661 711 BsaBI GATnn'nnATC Cuts at: 5068 5068 Size: 5074 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2881 3263 3898 275 Size: 53 83 186 936 1348 382 635 1451 Fragments arranged by size: 1451 1348 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3507 3905 4179 4326 4737 3507 Size: 398 274 147 411 3844 Fragments arranged by size: 3844 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4849 Fragments arranged by size: 4849 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2033 2341 1186 Size: 844 3 308 3919 Fragments arranged by size: 3919 844 308 3 BseYI C'CCAG_C Cuts at: 2131 4277 4773 2131 Size: 2146 496 2432 Fragments arranged by size: 2432 2146 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4501 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 2524 3285 4313 1540 Size: 275 186 322 201 761 1028 2301 Fragments arranged by size: 2301 1028 761 322 275 201 186 BsiHKAI G_wGCw'C Cuts at: 3135 3220 4291 3135 Size: 85 1071 3918 Fragments arranged by size: 3918 1071 85 BsmI GAATG_Cn' Cuts at: 2585 2678 2585 Size: 93 4981 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2138 2317 2393 3135 3220 4291 Size: 399 286 62 179 76 742 85 1071 Cuts at: 4291 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 286 179 85 76 62 BspEI T'CCGG_A Cuts at: 4737 4737 Size: 5074 BspHI T'CATG_A Cuts at: 2963 4693 2963 Size: 1730 3344 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2429 844 Size: 1292 293 3489 Fragments arranged by size: 3489 1292 293 BsrBI CCG'CTC Cuts at: 1394 1823 2368 2961 1394 Size: 429 545 593 3507 Fragments arranged by size: 3507 593 545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3569 3743 65 Size: 1183 2321 174 1396 Fragments arranged by size: 2321 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 2309 3718 3905 1700 Size: 110 273 226 1409 187 2869 Fragments arranged by size: 2869 1409 273 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5074 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5074 BssSI C'ACGA_G Cuts at: 3134 4146 3134 Size: 1012 4062 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5074 BstBI TT'CG_AA Cuts at: 3878 3878 Size: 5074 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5074 BstYI r'GATC_y Cuts at: 1972 2332 2404 2771 3156 3173 4625 4711 4723 Size: 360 72 367 385 17 1452 86 12 Cuts at: 4723 5069 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 360 346 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 513 Size: 552 939 45 3538 Fragments arranged by size: 3538 939 552 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4335 Fragments arranged by size: 4335 683 56 BtsI GCAGTG_nn' Cuts at: 2590 3384 3404 2590 Size: 794 20 4260 Fragments arranged by size: 4260 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5074 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5039 DraI TTT'AAA Cuts at: 2378 2378 Size: 5074 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4081 809 Size: 507 2765 1802 Fragments arranged by size: 2765 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2521 3410 Size: 54 1425 50 275 18 255 436 889 Cuts at: 3410 5039 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 436 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2521 1537 Size: 275 709 4090 Fragments arranged by size: 4090 709 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3004 1353 Size: 1651 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3661 4035 4181 387 Size: 1064 138 2072 374 146 1280 Fragments arranged by size: 2072 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3137 3734 4521 4789 1239 Size: 393 1505 597 787 268 1524 Fragments arranged by size: 1524 1505 787 597 393 268 EcoRI G'AATT_C Cuts at: 2383 2761 2383 Size: 378 4696 FspI TGC'GCA Cuts at: 2267 3580 2267 Size: 1313 3761 FspAI rTGC'GCAy Cuts at: 2267 2267 Size: 5074 HaeII r_GCGC'y Cuts at: 1678 2022 4221 1678 Size: 344 2199 2531 Fragments arranged by size: 2531 2199 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 3715 3747 3789 Size: 32 135 32 698 32 1413 32 42 Cuts at: 3789 3821 1373 Size: 32 2626 Fragments arranged by size: 2626 1413 698 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2418 2664 669 Size: 988 281 480 246 3079 Fragments arranged by size: 3079 988 480 281 246 HindIII A'AGCT_T Cuts at: 748 2230 748 Size: 1482 3592 HpaI GTT'AAC Cuts at: 2664 2664 Size: 5074 KpnI G_GTAC'C Cuts at: 2399 2399 Size: 5074 MfeI C'AATT_G Cuts at: 2673 2673 Size: 5074 MluI A'CGCG_T Cuts at: 4950 4950 Size: 5074 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4188 4372 1305 Size: 2883 184 2007 Fragments arranged by size: 2883 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3252 Fragments arranged by size: 3252 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3032 3391 3550 518 Size: 648 1866 359 159 2042 Fragments arranged by size: 2042 1866 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2490 3167 4315 4560 1606 Size: 445 439 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 445 439 245 NaeI GCC'GGC Cuts at: 2085 2085 Size: 5074 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3583 Fragments arranged by size: 3583 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5074 NgoMIV G'CCGG_C Cuts at: 2083 2083 Size: 5074 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5074 NotI GC'GGCC_GC Cuts at: 2521 2521 Size: 5074 NspI r_CATG'y Cuts at: 1132 1229 2144 2432 3977 1132 Size: 97 915 288 1545 2229 Fragments arranged by size: 2229 1545 915 288 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5074 PciI A'CATG_T Cuts at: 3973 3973 Size: 5074 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5074 PfoI T'CCnGG_A Cuts at: 2328 2328 Size: 5074 PmeI GTTT'AAAC Cuts at: 2378 2378 Size: 5074 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3143 3175 4656 4688 3143 Size: 32 1481 32 3529 Fragments arranged by size: 3529 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5074 PsiI TTA'TAA Cuts at: 2644 2644 Size: 5074 PspOMI G'GGCC_C Cuts at: 2134 2134 Size: 5074 PstI C_TGCA'G Cuts at: 830 2426 830 Size: 1596 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5074 RsrII CG'GwC_CG Cuts at: 2370 2370 Size: 5074 SacII CC_GC'GG Cuts at: 2052 2052 Size: 5074 SalI G'TCGA_C Cuts at: 1936 2416 1936 Size: 480 4594 SbfI CC_TGCA'GG Cuts at: 2426 2426 Size: 5074 ScaI AGT'ACT Cuts at: 3322 5034 3322 Size: 1712 3362 SfcI C'TryA_G Cuts at: 826 1045 2422 3557 4238 4429 826 Size: 219 1377 1135 681 191 1471 Fragments arranged by size: 1471 1377 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5074 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5074 SmaI CCC'GGG Cuts at: 1872 2401 1872 Size: 529 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3178 4079 4341 820 Size: 197 926 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2865 493 Size: 2372 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5074 SphI G_CATG'C Cuts at: 1132 2432 1132 Size: 1300 3774 SspI AAT'ATT Cuts at: 5 52 2998 5 Size: 47 2946 2081 Fragments arranged by size: 2946 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5074 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2513 513 Size: 552 939 84 425 3074 Fragments arranged by size: 3074 939 552 425 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3101 3118 3271 3456 3901 325 Size: 1255 1521 17 153 185 445 1498 Fragments arranged by size: 1521 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3320 5032 96 Size: 274 80 33 51 1443 1343 1712 138 Fragments arranged by size: 1712 1443 1343 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5034 5066 5034 Size: 32 5042 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5074 XbaI T'CTAG_A Cuts at: 2410 2410 Size: 5074 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5074 XmaI C'CCGG_G Cuts at: 1870 2399 1870 Size: 529 4545 XmnI GAAnn'nnTTC Cuts at: 3203 3203 Size: 5074 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2882 276 Size: 53 83 186 936 1348 2468 Fragments arranged by size: 2468 1348 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI