Restriction map for
pFN21AE6504
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiHKAI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DdeI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I TspRI
Tth111I Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 12 GAATTT [GA]^AATT[CT]
AlwNI 461 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 163 GGGCCC GGGCC^C
ApoI 12 GAATTT [GA]^AATT[CT]
BanII 163 GGGCCC G[GA]GC[CT]^C
BglI 156 GCCGTACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 163 GGGCCC G[AGT]GC[ACT]^C
BpmI 270 CTCCAG CTGGAG(16/14)
BsaI 236 GGTCTC GGTCTC(1/5)
BsiEI 109 CGGCCG CG[GA][CT]^CG
BsmBI 125 GAGACG CGTCTC(1/5)
Bsp1286I 163 GGGCCC G[AGT]GC[ACT]^C
BsrFI 227 GCCGGT [GA]^CCGG[CT]
BsrI 460 CCAGT ACTGG(1/-1)
BstYI 438 AGATCC [GA]^GATC[CT]
Cac8I 448 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 227 GCCGGT [GA]^CCGG[CT]
EaeI 109 CGGCCG [CT]^GGCC[GA]
EagI 109 CGGCCG C^GGCCG
EclXI 109 CGGCCG C^GGCCG
Eco52I 109 CGGCCG C^GGCCG
EcoNI 266 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 138 GAGTC G^A[ACGT]TC
KspI 281 CCGCGG CCGC^GG
MaeIII 191 GTCAC ^GT[ACGT]AC
MflI 438 AGATCC [GA]^GATC[CT]
MspA1I 281 CCGCGG C[AC]G^C[GT]G
PaeR7I 122 CTCGAG C^TCGAG
PleI 138 GAGTC GAGTC(4/5)
SacII 281 CCGCGG CCGC^GG
SfcI 467 CTGTAG C^T[GA][CT]AG
SmlI 122 CTCGAG C^T[CT][GA]AG
TaqI 123 TCGA T^CGA
Tsp45I 191 GTCAC ^GT[GC]AC
TthHB8I 123 TCGA T^CGA
XhoI 122 CTCGAG C^TCGAG
XhoII 438 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 200 CGCG CG^CG
282 CGCG
AluI 76 AGCT AG^CT
214 AGCT
AlwI 439 GATCC GGATC(4/5)
367 GGATC
BfaI 173 CTAG C^TAG
454 CTAG
BseRI 29 CTCCTC GAGGAG(10/8)
53 GAGGAG
BsiWI 158 CGTACG C^GTACG
179 CGTACG
BsmAI 125 GAGAC GTCTC(1/5)
237 GTCTC
BstUI 200 CGCG CG^CG
282 CGCG
DpnII 368 GATC ^GATC
439 GATC
DsaI 106 CCACGG C^C[GA][CT]GG
281 CCGCGG
EcoT14I 346 CCAAGG C^C[AT][AT]GG
442 CCTTGG
MaeI 173 CTAG C^TAG
454 CTAG
MboI 368 GATC ^GATC
439 GATC
MboII 436 GAAGA GAAGA(8/7)
524 GAAGA
MvnI 200 CGCG CG^CG
282 CGCG
NdeII 368 GATC ^GATC
439 GATC
PpuMI 274 AGGTCCC [GA]G^G[AT]CC[CT]
286 GGGTCCC
Sau3AI 368 GATC ^GATC
439 GATC
SmaI 90 CCCGGG CCC^GGG
219 CCCGGG
StyI 346 CCAAGG C^C[AT][AT]GG
442 CCTTGG
XmaCI 90 CCCGGG C^CCGGG
219 CCCGGG
XmaI 90 CCCGGG C^CCGGG
219 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AvaI AvaII BbvI
BcnI BsaJI BsiYI BslI BsmFI
BsoBI BssKI BstNI Cfr13I DraII
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HpaII ItaI MnlI MspI
MvaI MwoI NciI NlaIV RsaI
Sau96I ScrFI TseI Tsp509I