(Circular) (Six-base) MAPSORT of: pFN21AE6504.seq Check: 3761 from: 1 to: 5242 pFN21AE6504 5242 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3052 278 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2585 1937 Size: 648 4594 Acc65I G'GTAC_C Cuts at: 2563 2563 Size: 5242 AclI AA'CG_TT Cuts at: 1563 3369 3742 1563 Size: 1806 373 3063 Fragments arranged by size: 3063 1806 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3305 4689 4957 1632 Size: 1673 1384 268 1917 Fragments arranged by size: 1917 1673 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5045 AflIII A'CryG_T Cuts at: 1203 4141 5118 1203 Size: 2938 977 1327 Fragments arranged by size: 2938 1327 977 AgeI A'CCGG_T Cuts at: 4073 4073 Size: 5242 AhdI GACnn_n'nnGTC Cuts at: 3971 3971 Size: 5242 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5242 AlwNI CAG_nnn'CTG Cuts at: 2471 2985 4557 2471 Size: 514 1572 3156 Fragments arranged by size: 3156 1572 514 ApaI G_GGCC'C Cuts at: 2172 2172 Size: 5242 ApaLI G'TGCA_C Cuts at: 3299 4455 3299 Size: 1156 4086 ApoI r'AATT_y Cuts at: 1493 2017 2551 2777 2929 5038 1493 Size: 524 534 226 152 2109 1697 Fragments arranged by size: 2109 1697 534 524 226 152 AseI AT'TA_AT Cuts at: 160 1032 3796 160 Size: 872 2764 1606 Fragments arranged by size: 2764 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2127 2224 2567 1870 Size: 73 152 32 97 343 4545 Fragments arranged by size: 4545 343 152 97 73 32 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2128 2161 2524 2557 2128 Size: 33 363 33 4813 Fragments arranged by size: 4813 363 33 33 BamHI G'GATC_C Cuts at: 2572 2939 2572 Size: 367 4875 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2563 4018 618 Size: 325 217 627 776 1455 1842 Fragments arranged by size: 1842 1455 776 627 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2561 1391 Size: 781 389 4072 Fragments arranged by size: 4072 781 389 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3433 3467 1903 Size: 34 50 34 1412 34 3678 Fragments arranged by size: 3678 1412 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3134 4344 3134 Size: 1210 4032 BclI T'GATC_A Cuts at: 1548 1548 Size: 5242 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2167 3853 136 Size: 107 122 71 631 605 495 1686 1525 Fragments arranged by size: 1686 1525 631 605 495 122 107 71 BglII A'GATC_T Cuts at: 5237 5237 Size: 5242 BlpI GC'TnA_GC Cuts at: 2659 2659 Size: 5242 Bme1580I G_kGCm'C Cuts at: 1790 2172 3303 4459 1790 Size: 382 1131 1156 2573 Fragments arranged by size: 2573 1156 1131 382 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5242 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2973 3931 442 Size: 416 53 83 399 24 1556 958 1753 Fragments arranged by size: 1753 1556 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5242 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2260 3902 1239 Size: 1021 1642 2579 Fragments arranged by size: 2579 1642 1021 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3367 4232 4530 3367 Size: 865 298 4079 Fragments arranged by size: 4079 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2247 3905 882 Size: 617 748 1658 2219 Fragments arranged by size: 2219 1658 748 617 BsaAI yAC'GTr Cuts at: 493 1204 3033 493 Size: 711 1829 2702 Fragments arranged by size: 2702 1829 711 BsaBI GATnn'nnATC Cuts at: 5236 5236 Size: 5242 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3049 3431 4066 275 Size: 53 83 186 936 1516 382 635 1451 Fragments arranged by size: 1516 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3675 4073 4347 4494 4905 3675 Size: 398 274 147 411 4012 Fragments arranged by size: 4012 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2252 2282 1178 Size: 30 165 30 849 30 4138 Fragments arranged by size: 4138 849 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 2073 1186 Size: 839 48 4355 Fragments arranged by size: 4355 839 48 BseYI C'CCAG_C Cuts at: 2295 4445 4941 2295 Size: 2150 496 2596 Fragments arranged by size: 2596 2150 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4669 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2117 2692 3453 4481 1540 Size: 275 186 116 575 761 1028 2301 Fragments arranged by size: 2301 1028 761 575 275 186 116 BsiHKAI G_wGCw'C Cuts at: 3303 3388 4459 3303 Size: 85 1071 4086 Fragments arranged by size: 4086 1071 85 BsiWI C'GTAC_G Cuts at: 2163 2184 2163 Size: 21 5221 BsmI GAATG_Cn' Cuts at: 2753 2846 2753 Size: 93 5149 BsmBI CGTCTCn'nnnn_ Cuts at: 2124 2124 Size: 5242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2561 3303 3388 4459 1391 Size: 399 382 389 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 389 382 85 BspEI T'CCGG_A Cuts at: 4905 4905 Size: 5242 BspHI T'CATG_A Cuts at: 3131 4861 3131 Size: 1730 3512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2597 844 Size: 1753 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3129 1394 Size: 429 1306 3507 Fragments arranged by size: 3507 1306 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3737 3911 65 Size: 1183 2489 174 1396 Fragments arranged by size: 2489 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2232 3886 4073 1700 Size: 110 422 1654 187 2869 Fragments arranged by size: 2869 1654 422 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5242 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5242 BssSI C'ACGA_G Cuts at: 3302 4314 3302 Size: 1012 4230 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5242 BstBI TT'CG_AA Cuts at: 4046 4046 Size: 5242 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5242 BstYI r'GATC_y Cuts at: 1972 2443 2572 2939 3324 3341 4793 4879 4891 Size: 471 129 367 385 17 1452 86 12 Cuts at: 4891 5237 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 471 385 367 346 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2111 2286 513 Size: 552 1046 175 3469 Fragments arranged by size: 3469 1046 552 175 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4503 Fragments arranged by size: 4503 683 56 BtsI GCAGTG_nn' Cuts at: 2758 3552 3572 2758 Size: 794 20 4428 Fragments arranged by size: 4428 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5207 DraI TTT'AAA Cuts at: 2546 2546 Size: 5242 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4249 809 Size: 507 2933 1802 Fragments arranged by size: 2933 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2114 2689 3578 Size: 54 1425 50 275 18 284 575 889 Cuts at: 3578 5207 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 575 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2114 2689 1537 Size: 275 302 575 4090 Fragments arranged by size: 4090 575 302 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3172 1353 Size: 1819 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2121 3829 4203 4349 387 Size: 1064 138 532 1708 374 146 1280 Fragments arranged by size: 1708 1280 1064 532 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2260 3305 3902 4689 4957 1239 Size: 393 628 1045 597 787 268 1524 Fragments arranged by size: 1524 1045 787 628 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2275 2275 Size: 5242 EcoO109I rG'GnC_Cy Cuts at: 2169 2280 2292 2169 Size: 111 12 5119 Fragments arranged by size: 5119 111 12 EcoRI G'AATT_C Cuts at: 2551 2929 2551 Size: 378 4864 FspI TGC'GCA Cuts at: 3748 3748 Size: 5242 HaeII r_GCGC'y Cuts at: 1678 4389 1678 Size: 2711 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3883 3915 3957 3989 1373 Size: 32 135 32 2311 32 42 32 2626 Fragments arranged by size: 2626 2311 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2586 2832 669 Size: 988 281 648 246 3079 Fragments arranged by size: 3079 988 648 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5242 HpaI GTT'AAC Cuts at: 2832 2832 Size: 5242 KpnI G_GTAC'C Cuts at: 2567 2567 Size: 5242 MfeI C'AATT_G Cuts at: 2841 2841 Size: 5242 MluI A'CGCG_T Cuts at: 5118 5118 Size: 5242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 4356 4540 1305 Size: 1086 1965 184 2007 Fragments arranged by size: 2007 1965 1086 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3420 Fragments arranged by size: 3420 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3200 3559 3718 518 Size: 648 2034 359 159 2042 Fragments arranged by size: 2042 2034 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2288 2658 3335 4483 4728 1606 Size: 682 370 677 1148 245 2120 Fragments arranged by size: 2120 1148 682 677 370 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4690 NdeI CA'TA_TG Cuts at: 387 387 Size: 5242 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5242 NotI GC'GGCC_GC Cuts at: 2689 2689 Size: 5242 NspI r_CATG'y Cuts at: 1132 1229 2600 4145 1132 Size: 97 1371 1545 2229 Fragments arranged by size: 2229 1545 1371 97 PasI CC'CwG_GG Cuts at: 1348 2318 2341 1348 Size: 970 23 4249 Fragments arranged by size: 4249 970 23 PciI A'CATG_T Cuts at: 4141 4141 Size: 5242 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5242 PmeI GTTT'AAAC Cuts at: 2546 2546 Size: 5242 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3311 3343 4824 4856 3311 Size: 32 1481 32 3697 Fragments arranged by size: 3697 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2280 2292 2280 Size: 12 5230 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5242 PsiI TTA'TAA Cuts at: 2812 2812 Size: 5242 PspOMI G'GGCC_C Cuts at: 2168 2168 Size: 5242 PstI C_TGCA'G Cuts at: 830 2594 830 Size: 1764 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5242 SacII CC_GC'GG Cuts at: 2289 2289 Size: 5242 SalI G'TCGA_C Cuts at: 1936 2584 1936 Size: 648 4594 SanDI GG'GwC_CC Cuts at: 2292 2292 Size: 5242 SbfI CC_TGCA'GG Cuts at: 2594 2594 Size: 5242 ScaI AGT'ACT Cuts at: 3490 5202 3490 Size: 1712 3530 SfcI C'TryA_G Cuts at: 826 1045 2472 2590 3725 4406 4597 826 Size: 219 1427 118 1135 681 191 1471 Fragments arranged by size: 1471 1427 1135 681 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5242 SmaI CCC'GGG Cuts at: 1872 2097 2226 2569 1872 Size: 225 129 343 4545 Fragments arranged by size: 4545 343 225 129 SmlI C'TyrA_G Cuts at: 820 1017 1943 2127 3346 4247 4509 820 Size: 197 926 184 1219 901 262 1553 Fragments arranged by size: 1553 1219 926 901 262 197 184 SnaBI TAC'GTA Cuts at: 493 3033 493 Size: 2540 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5242 SphI G_CATG'C Cuts at: 1132 2600 1132 Size: 1468 3774 SspI AAT'ATT Cuts at: 5 52 3166 5 Size: 47 3114 2081 Fragments arranged by size: 3114 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5242 StyI C'CwwG_G Cuts at: 513 1065 2351 2447 2681 513 Size: 552 1286 96 234 3074 Fragments arranged by size: 3074 1286 552 234 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3269 3286 3439 3624 4069 325 Size: 1255 1689 17 153 185 445 1498 Fragments arranged by size: 1689 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3488 5200 96 Size: 274 80 33 51 1443 1511 1712 138 Fragments arranged by size: 1712 1511 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5202 5234 5202 Size: 32 5210 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5242 XbaI T'CTAG_A Cuts at: 2578 2578 Size: 5242 XhoI C'TCGA_G Cuts at: 1943 2127 1943 Size: 184 5058 XmaI C'CCGG_G Cuts at: 1870 2095 2224 2567 1870 Size: 225 129 343 4545 Fragments arranged by size: 4545 343 225 129 XmnI GAAnn'nnTTC Cuts at: 3371 3371 Size: 5242 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3050 276 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI