Restriction map for
pFN21AE6491
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsiWI Bsp1407I BspDI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MscI MseI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AccI 673 GTCTAC GT^[AC][GT]AC
AclI 343 AACGTT AA^CGTT
AviII 1068 TGCGCA TGC^GCA
BbsI 460 GTCTTC GAAGAC(2/6)
BpuAI 460 GTCTTC GAAGAC(2/6)
BsaAI 1785 TACGTG [CT]AC^GT[GA]
BsmI 621 GAATGC GAATGC(1/-1)
BsrDI 948 CATTGC GCAATG(2/0)
BstBI 1348 TTCGAA TT^CGAA
DrdI 535 GACATCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 551 CCGTGG C^C[GA][CT]GG
EarI 157 CTCTTC CTCTTC(1/4)
EcoNI 1605 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1068 TGCGCA TGC^GCA
HinfI 1189 GAGTC G^A[ACGT]TC
Ksp632I 157 CTCTTC CTCTTC(1/4)
NspV 1348 TTCGAA TT^CGAA
PleI 1189 GAGTC GAGTC(4/5)
Psp1406I 343 AACGTT AA^CGTT
PvuII 115 CAGCTG CAG^CTG
SacI 1644 GAGCTC GAGCT^C
SfcI 402 CTACAG C^T[GA][CT]AG
SfiI 990 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1348 TTCGAA TT^CGAA
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccIII 161 TCCGGA T^CCGGA
1192 TCCGGA
AlwI 1577 GGATC GGATC(4/5)
1790 GGATC
AocI 898 CCTGAGG CC^T[ACGT]AGG
1015 CCTGAGG
AspI 1423 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1669 GACATGGTC
BbeI 3 GGCGCC GGCGC^C
834 GGCGCC
BfaI 1084 CTAG C^TAG
1342 CTAG
BglI 991 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1126 GCCTCGGAGGC
BglII 398 AGATCT A^GATCT
1235 AGATCT
BsaI 614 GAGACC GGTCTC(1/5)
926 GGTCTC
BsaWI 161 TCCGGA [AT]^CCGG[AT]
1192 TCCGGA
BseAI 161 TCCGGA T^CCGGA
1192 TCCGGA
BseRI 394 GAGGAG GAGGAG(10/8)
1510 GAGGAG
BsgI 652 CTGCAC GTGCAG(16/14)
1738 CTGCAC
BsiEI 258 CGGCCG CG[GA][CT]^CG
1704 CGGCCG
BsmBI 765 CGTCTC CGTCTC(1/5)
1099 GAGACG
BspEI 161 TCCGGA T^CCGGA
1192 TCCGGA
BspHI 497 TCATGA T^CATGA
1676 TCATGA
BsrI 329 ACTGG ACTGG(1/-1)
1314 CCAGT
BstAPI 1055 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1655 GCAAGAGCTGC
Bsu36I 898 CCTGAGG CC^T[ACGT]AGG
1015 CCTGAGG
EagI 258 CGGCCG C^GGCCG
1704 CGGCCG
EclXI 258 CGGCCG C^GGCCG
1704 CGGCCG
Eco52I 258 CGGCCG C^GGCCG
1704 CGGCCG
Eco81I 898 CCTGAGG CC^T[ACGT]AGG
1015 CCTGAGG
HgaI 442 GACGC GACGC(5/10)
1381 GACGC
HincII 1159 GTCAAC GT[CT]^[GA]AC
1714 GTCAAC
HindII 1159 GTCAAC GT[CT]^[GA]AC
1714 GTCAAC
HphI 784 GGTGA GGTGA(8/7)
1254 GGTGA
KasI 3 GGCGCC G^GCGCC
834 GGCGCC
MaeI 1084 CTAG C^TAG
1342 CTAG
MroI 161 TCCGGA T^CCGGA
1192 TCCGGA
NarI 3 GGCGCC GG^CGCC
834 GGCGCC
NotI 257 GCGGCCGC GC^GGCCGC
1703 GCGGCCGC
PflFI 1423 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1669 GACATGGTC
PshAI 1299 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1608 GACTGAGGTC
RcaI 497 TCATGA T^CATGA
1676 TCATGA
SfaNI 492 GATGC GCATC(5/9)
592 GATGC
SfoI 3 GGCGCC GGC^GCC
834 GGCGCC
Tth111I 1423 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1669 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SexAI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI