(Circular) (Six-base) MAPSORT of: pFN21AE6491.seq Check: 2839 from: 1 to: 6505 pFN21AE6491 6505 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2777 2837 3151 2777 Size: 60 314 6131 Fragments arranged by size: 6131 314 60 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4315 278 Size: 53 83 186 936 2779 2468 Fragments arranged by size: 2779 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2679 3848 1937 Size: 742 1169 4594 Fragments arranged by size: 4594 1169 742 Acc65I G'GTAC_C Cuts at: 3826 3826 Size: 6505 AclI AA'CG_TT Cuts at: 1563 2349 4632 5005 1563 Size: 786 2283 373 3063 Fragments arranged by size: 3063 2283 786 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3782 4568 5952 6220 1632 Size: 2150 786 1384 268 1917 Fragments arranged by size: 2150 1917 1384 786 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6308 AflIII A'CryG_T Cuts at: 1203 5404 6381 1203 Size: 4201 977 1327 Fragments arranged by size: 4201 1327 977 AgeI A'CCGG_T Cuts at: 5336 5336 Size: 6505 AhdI GACnn_n'nnGTC Cuts at: 5234 5234 Size: 6505 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6505 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6505 AlwNI CAG_nnn'CTG Cuts at: 2829 2898 3066 3325 3645 4248 5820 2829 Size: 69 168 259 320 603 1572 3514 Fragments arranged by size: 3514 1572 603 320 259 168 69 ApaLI G'TGCA_C Cuts at: 4562 5718 4562 Size: 1156 5349 ApoI r'AATT_y Cuts at: 1493 3814 4040 4192 6301 1493 Size: 2321 226 152 2109 1697 Fragments arranged by size: 2321 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5059 160 Size: 872 4027 1606 Fragments arranged by size: 4027 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2181 3114 3830 1870 Size: 73 120 118 933 716 4545 Fragments arranged by size: 4545 933 716 120 118 73 BamHI G'GATC_C Cuts at: 3835 4202 3835 Size: 367 6138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2839 3109 3139 3694 Size: 325 217 627 221 831 270 30 555 Cuts at: 3694 3826 5281 618 Size: 132 1455 1842 Fragments arranged by size: 1842 1455 831 627 555 325 270 221 217 132 30 BanII G_rGCy'C Cuts at: 1391 2611 2862 2870 2943 3087 3653 3824 1391 Size: 1220 251 8 73 144 566 171 4072 Fragments arranged by size: 4072 1220 566 251 171 144 73 8 BbeI G_GCGC'C Cuts at: 2012 2843 2012 Size: 831 5674 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2458 928 Size: 971 559 4975 Fragments arranged by size: 4975 971 559 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4696 4730 1903 Size: 34 2759 34 3678 Fragments arranged by size: 3678 2759 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4397 5607 4397 Size: 1210 5295 BclI T'GATC_A Cuts at: 1548 1548 Size: 6505 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3002 3137 5116 Size: 107 122 71 631 605 1330 135 1979 Cuts at: 5116 136 Size: 1525 Fragments arranged by size: 1979 1525 1330 631 605 135 122 107 71 BglII A'GATC_T Cuts at: 2403 3240 6500 2403 Size: 837 3260 2408 Fragments arranged by size: 3260 2408 837 BlpI GC'TnA_GC Cuts at: 3922 3922 Size: 6505 Bme1580I G_kGCm'C Cuts at: 1790 3047 4566 5722 1790 Size: 1257 1519 1156 2573 Fragments arranged by size: 2573 1519 1257 1156 BmgBI CAC'GTC Cuts at: 1318 2110 1318 Size: 792 5713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4236 5194 442 Size: 416 53 83 399 24 2819 958 1753 Fragments arranged by size: 2819 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6505 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 3212 3461 3667 5165 1239 Size: 905 1068 249 206 1498 2579 Fragments arranged by size: 2579 1498 1068 905 249 206 Bpu10I CC'TnA_GC Cuts at: 2856 2937 2856 Size: 81 6424 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2099 2183 2348 2699 3539 4630 5495 5793 2099 Size: 84 165 351 840 1091 865 298 2811 Fragments arranged by size: 2811 1091 865 840 351 298 165 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2613 2937 5168 882 Size: 617 1114 324 2231 2219 Fragments arranged by size: 2231 2219 1114 617 324 BsaAI yAC'GTr Cuts at: 493 1204 3792 4296 493 Size: 711 2588 504 2702 Fragments arranged by size: 2702 2588 711 504 BsaBI GATnn'nnATC Cuts at: 6499 6499 Size: 6505 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2448 2840 4312 Size: 53 83 186 936 476 439 392 1472 Cuts at: 4312 4694 5329 275 Size: 382 635 1451 Fragments arranged by size: 1472 1451 936 635 476 439 392 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2166 3197 4938 5336 5610 5757 6168 2166 Size: 1031 1741 398 274 147 411 2503 Fragments arranged by size: 2503 1741 1031 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 2294 2324 3209 Size: 30 165 30 858 30 3 30 885 Cuts at: 3209 3227 3239 3257 1178 Size: 18 12 18 4426 Fragments arranged by size: 4426 885 858 165 30 30 30 30 18 18 12 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 3530 1186 Size: 1228 1116 4161 Fragments arranged by size: 4161 1228 1116 BseYI C'CCAG_C Cuts at: 2012 2042 2441 2891 2914 5708 6204 2012 Size: 30 399 450 23 2794 496 2313 Fragments arranged by size: 2794 2313 496 450 399 30 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2642 3728 1163 Size: 573 906 1086 3940 Fragments arranged by size: 3940 1086 906 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2266 3712 3955 4716 5744 1540 Size: 275 186 265 1446 243 761 1028 2301 Fragments arranged by size: 2301 1446 1028 761 275 265 243 186 BsiHKAI G_wGCw'C Cuts at: 2109 3653 3758 4566 4651 5722 2109 Size: 1544 105 808 85 1071 2892 Fragments arranged by size: 2892 1544 1071 808 105 85 BsmI GAATG_Cn' Cuts at: 2632 4016 4109 2632 Size: 1384 93 5028 Fragments arranged by size: 5028 1384 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2776 3098 2776 Size: 322 6183 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2611 2862 2870 2943 3047 3087 Size: 399 319 502 251 8 73 104 40 Cuts at: 3087 3653 3758 3824 4566 4651 5722 1391 Size: 566 105 66 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 566 502 399 319 251 105 104 85 73 66 40 8 BspEI T'CCGG_A Cuts at: 2166 3197 6168 2166 Size: 1031 2971 2503 Fragments arranged by size: 2971 2503 1031 BspHI T'CATG_A Cuts at: 2502 3681 4394 6124 2502 Size: 1179 713 1730 2883 Fragments arranged by size: 2883 1730 1179 713 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2368 2777 2837 3151 3291 3860 844 Size: 1524 409 60 314 140 569 3489 Fragments arranged by size: 3489 1524 569 409 314 140 60 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2049 2128 2484 3301 4392 1394 Size: 429 206 20 79 356 817 1091 3507 Fragments arranged by size: 3507 1091 817 429 356 206 79 20 BsrDI GCAATG_nn' Cuts at: 65 1248 2952 5000 5174 65 Size: 1183 1704 2048 174 1396 Fragments arranged by size: 2048 1704 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5149 5336 1700 Size: 110 3339 187 2869 Fragments arranged by size: 3339 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6505 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6505 BssSI C'ACGA_G Cuts at: 4565 5577 4565 Size: 1012 5493 BstAPI GCAn_nnn'nTGC Cuts at: 794 3066 3666 794 Size: 2272 600 3633 Fragments arranged by size: 3633 2272 600 BstBI TT'CG_AA Cuts at: 3354 5309 3354 Size: 1955 4550 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6505 BstYI r'GATC_y Cuts at: 1972 2403 3240 3582 3835 4202 4587 4604 6056 Size: 431 837 342 253 367 385 17 1452 Cuts at: 6056 6142 6154 6500 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 837 431 385 367 346 342 253 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2904 3021 2904 Size: 117 6388 BtgI C'CryG_G Cuts at: 513 1065 2004 2556 513 Size: 552 939 552 4462 Fragments arranged by size: 4462 939 552 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5766 Fragments arranged by size: 5766 683 56 BtsI GCAGTG_nn' Cuts at: 2299 3646 4021 4815 4835 2299 Size: 1347 375 794 20 3969 Fragments arranged by size: 3969 1347 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6505 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6470 DraI TTT'AAA Cuts at: 3809 3809 Size: 6505 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2546 5512 809 Size: 507 1230 2966 1802 Fragments arranged by size: 2966 1802 1230 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2263 2487 2559 Size: 54 1425 50 275 18 433 224 72 Cuts at: 2559 3274 3709 3952 4841 6470 8 Size: 715 435 243 889 1629 43 Fragments arranged by size: 1629 1425 889 715 435 433 275 243 224 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2263 3709 3952 1537 Size: 275 451 1446 243 4090 Fragments arranged by size: 4090 1446 451 275 243 EarI CTCTTCn'nnn_ Cuts at: 1353 2168 4435 1353 Size: 815 2267 3423 Fragments arranged by size: 3423 2267 815 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2978 3368 5092 5466 5612 387 Size: 1064 138 1389 390 1724 374 146 1280 Fragments arranged by size: 1724 1389 1280 1064 390 374 146 138 EcoICRI GAG'CTC Cuts at: 3651 3651 Size: 6505 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 3212 3461 3667 3782 4568 5165 Size: 393 512 1068 249 206 115 786 597 Cuts at: 5165 5952 6220 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1068 787 786 597 512 393 268 249 206 115 EcoNI CCTnn'n_nnAGG Cuts at: 3614 3614 Size: 6505 EcoO109I rG'GnC_Cy Cuts at: 3016 3050 3466 3568 3016 Size: 34 416 102 5953 Fragments arranged by size: 5953 416 102 34 EcoRI G'AATT_C Cuts at: 3814 4192 3814 Size: 378 6127 FspI TGC'GCA Cuts at: 3075 5011 3075 Size: 1936 4569 FspAI rTGC'GCAy Cuts at: 3075 3075 Size: 6505 HaeII r_GCGC'y Cuts at: 1678 2012 2843 3743 5652 1678 Size: 334 831 900 1909 2531 Fragments arranged by size: 2531 1909 900 831 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 3227 3259 5146 Size: 32 135 32 722 32 901 32 1887 Cuts at: 5146 5178 5220 5252 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1887 901 722 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3166 3721 3849 4095 669 Size: 988 281 1228 555 128 246 3079 Fragments arranged by size: 3079 1228 988 555 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 6505 HpaI GTT'AAC Cuts at: 4095 4095 Size: 6505 KasI G'GCGC_C Cuts at: 2008 2839 2008 Size: 831 5674 KpnI G_GTAC'C Cuts at: 3830 3830 Size: 6505 MfeI C'AATT_G Cuts at: 4104 4104 Size: 6505 MluI A'CGCG_T Cuts at: 6381 6381 Size: 6505 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3207 3553 5619 5803 1305 Size: 1902 346 2066 184 2007 Fragments arranged by size: 2066 2007 1902 346 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4683 Fragments arranged by size: 4683 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2507 2513 3680 4463 4822 4981 518 Size: 648 1341 6 1167 783 359 159 2042 Fragments arranged by size: 2042 1341 1167 783 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2037 2122 2142 2580 3066 3636 3708 3921 Size: 431 85 20 438 486 570 72 213 Cuts at: 3921 4598 5746 5991 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 570 486 438 431 245 213 85 72 20 NarI GG'CG_CC Cuts at: 2009 2840 2009 Size: 831 5674 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5014 Fragments arranged by size: 5014 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6505 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6505 NotI GC'GGCC_GC Cuts at: 2263 3709 3952 2263 Size: 1446 243 4816 Fragments arranged by size: 4816 1446 243 NspI r_CATG'y Cuts at: 1132 1229 3863 5408 1132 Size: 97 2634 1545 2229 Fragments arranged by size: 2634 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6505 PciI A'CATG_T Cuts at: 5404 5404 Size: 6505 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6505 PfoI T'CCnGG_A Cuts at: 2391 2391 Size: 6505 PmeI GTTT'AAAC Cuts at: 3809 3809 Size: 6505 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4574 4606 6087 6119 4574 Size: 32 1481 32 4960 Fragments arranged by size: 4960 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3016 3050 3466 3568 3016 Size: 34 416 102 5953 Fragments arranged by size: 5953 416 102 34 PshAI GACnn'nnGTC Cuts at: 1613 3308 3617 1613 Size: 1695 309 4501 Fragments arranged by size: 4501 1695 309 PsiI TTA'TAA Cuts at: 4075 4075 Size: 6505 PstI C_TGCA'G Cuts at: 830 3857 830 Size: 3027 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6505 PvuII CAG'CTG Cuts at: 2122 2122 Size: 6505 SacI G_AGCT'C Cuts at: 3653 3653 Size: 6505 SalI G'TCGA_C Cuts at: 1936 3847 1936 Size: 1911 4594 SanDI GG'GwC_CC Cuts at: 3016 3016 Size: 6505 SbfI CC_TGCA'GG Cuts at: 3857 3857 Size: 6505 ScaI AGT'ACT Cuts at: 4753 6465 4753 Size: 1712 4793 SexAI A'CCwGG_T Cuts at: 2670 2724 3462 2670 Size: 54 738 5713 Fragments arranged by size: 5713 738 54 SfcI C'TryA_G Cuts at: 826 1045 2407 3853 4988 5669 5860 826 Size: 219 1362 1446 1135 681 191 1471 Fragments arranged by size: 1471 1446 1362 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3002 3002 Size: 6505 SfoI GGC'GCC Cuts at: 2010 2841 2010 Size: 831 5674 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6505 SmaI CCC'GGG Cuts at: 1872 2065 2183 3116 3832 1872 Size: 193 118 933 716 4545 Fragments arranged by size: 4545 933 716 193 118 SmlI C'TyrA_G Cuts at: 820 1017 1943 2114 2198 2363 2714 3554 4609 Size: 197 926 171 84 165 351 840 1055 Cuts at: 4609 5510 5772 820 Size: 901 262 1553 Fragments arranged by size: 1553 1055 926 901 840 351 262 197 171 165 84 SnaBI TAC'GTA Cuts at: 493 4296 493 Size: 3803 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6505 SphI G_CATG'C Cuts at: 1132 3863 1132 Size: 2731 3774 SspI AAT'ATT Cuts at: 5 52 4429 5 Size: 47 4377 2081 Fragments arranged by size: 4377 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6505 StyI C'CwwG_G Cuts at: 513 1065 2004 2454 2964 3006 3944 513 Size: 552 939 450 510 42 938 3074 Fragments arranged by size: 3074 939 938 552 510 450 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2628 4532 4549 4702 4887 5332 325 Size: 1255 1048 1904 17 153 185 445 1498 Fragments arranged by size: 1904 1498 1255 1048 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2331 3180 4751 Size: 274 80 33 51 1443 354 849 1571 Cuts at: 4751 6463 96 Size: 1712 138 Fragments arranged by size: 1712 1571 1443 849 354 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6465 6497 6465 Size: 32 6473 Tth111I GACn'n_nGTC Cuts at: 1316 3431 3677 1316 Size: 2115 246 4144 Fragments arranged by size: 4144 2115 246 XbaI T'CTAG_A Cuts at: 3841 3841 Size: 6505 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2671 2752 2971 3556 2671 Size: 81 219 585 5620 Fragments arranged by size: 5620 585 219 81 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6505 XmaI C'CCGG_G Cuts at: 1870 2063 2181 3114 3830 1870 Size: 193 118 933 716 4545 Fragments arranged by size: 4545 933 716 193 118 XmnI GAAnn'nnTTC Cuts at: 4634 4634 Size: 6505 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4313 276 Size: 53 83 186 936 2779 2468 Fragments arranged by size: 2779 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SrfI SwaI