(Linear) (Six-base) MAPSORT of: KIEE6491.seq Check: 2034 from: 1 to: 1800 KIEE6491.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 772 832 1146 1800 Size: 772 60 314 654 Fragments arranged by size: 772 654 314 60 AccI GT'mk_AC Cuts at: 0 674 1800 Size: 674 1126 AclI AA'CG_TT Cuts at: 0 344 1800 Size: 344 1456 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1777 1800 Size: 1777 23 AlwNI CAG_nnn'CTG Cuts at: 0 824 893 1061 1320 1640 1800 Size: 824 69 168 259 320 160 Fragments arranged by size: 824 320 259 168 160 69 AvaI C'yCGr_G Cuts at: 0 58 176 1109 1800 Size: 58 118 933 691 Fragments arranged by size: 933 691 118 58 BanI G'GyrC_C Cuts at: 0 3 834 1104 1134 1689 1800 Size: 3 831 270 30 555 111 Fragments arranged by size: 831 555 270 111 30 3 BanII G_rGCy'C Cuts at: 0 606 857 865 938 1082 1648 1800 Size: 606 251 8 73 144 566 152 Fragments arranged by size: 606 566 251 152 144 73 8 BbeI G_GCGC'C Cuts at: 0 7 838 1800 Size: 7 831 962 Fragments arranged by size: 962 831 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 453 1800 Size: 453 1347 BglI GCCn_nnn'nGGC Cuts at: 0 997 1132 1800 Size: 997 135 668 Fragments arranged by size: 997 668 135 BglII A'GATC_T Cuts at: 0 398 1235 1800 Size: 398 837 565 Fragments arranged by size: 837 565 398 Bme1580I G_kGCm'C Cuts at: 0 1042 1800 Size: 1042 758 BmgBI CAC'GTC Cuts at: 0 105 1800 Size: 105 1695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 1207 1456 1662 1800 Size: 139 1068 249 206 138 Fragments arranged by size: 1068 249 206 139 138 Bpu10I CC'TnA_GC Cuts at: 0 851 932 1800 Size: 851 81 868 Fragments arranged by size: 868 851 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 178 343 694 1534 1800 Size: 94 84 165 351 840 266 Fragments arranged by size: 840 351 266 165 94 84 BsaI GGTCTCn'nnnn_ Cuts at: 0 608 932 1800 Size: 608 324 868 Fragments arranged by size: 868 608 324 BsaAI yAC'GTr Cuts at: 0 1787 1800 Size: 1787 13 BsaHI Gr'CG_yC Cuts at: 0 4 443 835 1800 Size: 4 439 392 965 Fragments arranged by size: 965 439 392 4 BsaWI w'CCGG_w Cuts at: 0 161 1192 1800 Size: 161 1031 608 Fragments arranged by size: 1031 608 161 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 256 286 289 319 1204 1222 1234 1252 Size: 256 30 3 30 885 18 12 18 Cuts at: 1252 1800 Size: 548 Fragments arranged by size: 885 548 256 30 30 18 18 12 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 409 1525 1800 Size: 409 1116 275 Fragments arranged by size: 1116 409 275 BseYI C'CCAG_C Cuts at: 0 7 37 436 886 909 1800 Size: 7 30 399 450 23 891 Fragments arranged by size: 891 450 399 30 23 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 1723 1800 Size: 637 1086 77 Fragments arranged by size: 1086 637 77 BsiEI CG_ry'CG Cuts at: 0 261 1707 1800 Size: 261 1446 93 Fragments arranged by size: 1446 261 93 BsiHKAI G_wGCw'C Cuts at: 0 104 1648 1753 1800 Size: 104 1544 105 47 Fragments arranged by size: 1544 105 104 47 BsmI GAATG_Cn' Cuts at: 0 627 1800 Size: 627 1173 BsmBI CGTCTCn'nnnn_ Cuts at: 0 771 1093 1800 Size: 771 322 707 Fragments arranged by size: 771 707 322 Bsp1286I G_dGCh'C Cuts at: 0 104 606 857 865 938 1042 1082 1648 Size: 104 502 251 8 73 104 40 566 Cuts at: 1648 1753 1800 Size: 105 47 Fragments arranged by size: 566 502 251 105 104 104 73 47 40 8 BspEI T'CCGG_A Cuts at: 0 161 1192 1800 Size: 161 1031 608 Fragments arranged by size: 1031 608 161 BspHI T'CATG_A Cuts at: 0 497 1676 1800 Size: 497 1179 124 Fragments arranged by size: 1179 497 124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 363 772 832 1146 1286 1800 Size: 363 409 60 314 140 514 Fragments arranged by size: 514 409 363 314 140 60 BsrBI CCG'CTC Cuts at: 0 24 44 123 479 1296 1800 Size: 24 20 79 356 817 504 Fragments arranged by size: 817 504 356 79 24 20 BsrDI GCAATG_nn' Cuts at: 0 947 1800 Size: 947 853 BstAPI GCAn_nnn'nTGC Cuts at: 0 1061 1661 1800 Size: 1061 600 139 Fragments arranged by size: 1061 600 139 BstBI TT'CG_AA Cuts at: 0 1349 1800 Size: 1349 451 BstYI r'GATC_y Cuts at: 0 398 1235 1577 1800 Size: 398 837 342 223 Fragments arranged by size: 837 398 342 223 Bsu36I CC'TnA_GG Cuts at: 0 899 1016 1800 Size: 899 117 784 Fragments arranged by size: 899 784 117 BtgI C'CryG_G Cuts at: 0 551 1800 Size: 551 1249 BtsI GCAGTG_nn' Cuts at: 0 294 1641 1800 Size: 294 1347 159 Fragments arranged by size: 1347 294 159 DrdI GACnn_nn'nnGTC Cuts at: 0 541 1800 Size: 541 1259 EaeI y'GGCC_r Cuts at: 0 258 482 554 1269 1704 1800 Size: 258 224 72 715 435 96 Fragments arranged by size: 715 435 258 224 96 72 EagI C'GGCC_G Cuts at: 0 258 1704 1800 Size: 258 1446 96 Fragments arranged by size: 1446 258 96 EarI CTCTTCn'nnn_ Cuts at: 0 163 1800 Size: 163 1637 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 973 1363 1800 Size: 973 390 437 Fragments arranged by size: 973 437 390 EcoICRI GAG'CTC Cuts at: 0 1646 1800 Size: 1646 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 1207 1456 1662 1777 1800 Size: 139 1068 249 206 115 23 Fragments arranged by size: 1068 249 206 139 115 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 1609 1800 Size: 1609 191 EcoO109I rG'GnC_Cy Cuts at: 0 1011 1045 1461 1563 1800 Size: 1011 34 416 102 237 Fragments arranged by size: 1011 416 237 102 34 FspI TGC'GCA Cuts at: 0 1070 1800 Size: 1070 730 FspAI rTGC'GCAy Cuts at: 0 1070 1800 Size: 1070 730 HaeII r_GCGC'y Cuts at: 0 7 838 1738 1800 Size: 7 831 900 62 Fragments arranged by size: 900 831 62 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 289 321 1222 1254 1800 Size: 289 32 901 32 546 Fragments arranged by size: 901 546 289 32 32 HincII GTy'rAC Cuts at: 0 1161 1716 1800 Size: 1161 555 84 Fragments arranged by size: 1161 555 84 KasI G'GCGC_C Cuts at: 0 3 834 1800 Size: 3 831 966 Fragments arranged by size: 966 831 3 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1202 1548 1800 Size: 1202 346 252 Fragments arranged by size: 1202 346 252 MslI CAynn'nnrTG Cuts at: 0 502 508 1675 1800 Size: 502 6 1167 125 Fragments arranged by size: 1167 502 125 6 MspA1I CmG'CkG Cuts at: 0 32 117 137 575 1061 1631 1703 1800 Size: 32 85 20 438 486 570 72 97 Fragments arranged by size: 570 486 438 97 85 72 32 20 NarI GG'CG_CC Cuts at: 0 4 835 1800 Size: 4 831 965 Fragments arranged by size: 965 831 4 NotI GC'GGCC_GC Cuts at: 0 258 1704 1800 Size: 258 1446 96 Fragments arranged by size: 1446 258 96 PfoI T'CCnGG_A Cuts at: 0 386 1800 Size: 386 1414 PpuMI rG'GwC_Cy Cuts at: 0 1011 1045 1461 1563 1800 Size: 1011 34 416 102 237 Fragments arranged by size: 1011 416 237 102 34 PshAI GACnn'nnGTC Cuts at: 0 1303 1612 1800 Size: 1303 309 188 Fragments arranged by size: 1303 309 188 PvuII CAG'CTG Cuts at: 0 117 1800 Size: 117 1683 SacI G_AGCT'C Cuts at: 0 1648 1800 Size: 1648 152 SanDI GG'GwC_CC Cuts at: 0 1011 1800 Size: 1011 789 SexAI A'CCwGG_T Cuts at: 0 665 719 1457 1800 Size: 665 54 738 343 Fragments arranged by size: 738 665 343 54 SfcI C'TryA_G Cuts at: 0 402 1800 Size: 402 1398 SfiI GGCCn_nnn'nGGCC Cuts at: 0 997 1800 Size: 997 803 SfoI GGC'GCC Cuts at: 0 5 836 1800 Size: 5 831 964 Fragments arranged by size: 964 831 5 SmaI CCC'GGG Cuts at: 0 60 178 1111 1800 Size: 60 118 933 689 Fragments arranged by size: 933 689 118 60 SmlI C'TyrA_G Cuts at: 0 109 193 358 709 1549 1800 Size: 109 84 165 351 840 251 Fragments arranged by size: 840 351 251 165 109 84 StyI C'CwwG_G Cuts at: 0 449 959 1001 1800 Size: 449 510 42 799 Fragments arranged by size: 799 510 449 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 623 1800 Size: 623 1177 TatI w'GTAC_w Cuts at: 0 326 1175 1800 Size: 326 849 625 Fragments arranged by size: 849 625 326 Tth111I GACn'n_nGTC Cuts at: 0 1426 1672 1800 Size: 1426 246 128 Fragments arranged by size: 1426 246 128 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 666 747 966 1551 1800 Size: 666 81 219 585 249 Fragments arranged by size: 666 585 249 219 81 XmaI C'CCGG_G Cuts at: 0 58 176 1109 1800 Size: 58 118 933 691 Fragments arranged by size: 933 691 118 58 Enzymes that do cut: AarI AccI AclI AcuI AlwNI AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstAPI BstBI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII KasI MmeI MslI MspA1I NarI NotI PfoI PpuMI PshAI PvuII SacI SanDI SexAI SfcI SfiI SfoI SmaI SmlI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaBI BsiWI BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XhoI XmnI ZraI