Restriction map for
pFN21AE6481
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI BbrPI BbvCI BcgI
BciVI BclI BfrI BlpI BpmI
Bpu1102I BseAI BsiWI BsmBI BspEI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MfeI MluI MroI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 514 GACGTC GACGT^C
AlwNI 42 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1359 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1162 GTGCTC G[AT]GC[AT]^C
AspI 163 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1433 TGCGCA TGC^GCA
AvrII 54 CCTAGG C^CTAGG
BamHI 1018 GGATCC G^GATCC
BbeI 938 GGCGCC GGCGC^C
BbsI 306 GAAGAC GAAGAC(2/6)
BlnI 54 CCTAGG C^CTAGG
BpuAI 306 GAAGAC GAAGAC(2/6)
BsaAI 1114 TACGTG [CT]AC^GT[GA]
BsaI 909 GGTCTC GGTCTC(1/5)
BsaWI 345 TCCGGT [AT]^CCGG[AT]
BsiEI 785 CGATCG CG[GA][CT]^CG
BsiHKAI 1162 GTGCTC G[AT]GC[AT]^C
BsmI 1463 GCATTC GAATGC(1/-1)
Bsp1407I 962 TGTACA T^GTACA
BspDI 295 ATCGAT AT^CGAT
BspHI 518 TCATGA T^CATGA
BsrDI 528 CATTGC GCAATG(2/0)
BsrGI 962 TGTACA T^GTACA
BstAPI 41 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 84 GGTCACC G^GT[ACGT]ACC
BstPI 84 GGTCACC G^GT[ACGT]ACC
ClaI 295 ATCGAT AT^CGAT
EarI 757 CTCTTC CTCTTC(1/4)
EcoO65I 84 GGTCACC G^GT[ACGT]ACC
FspI 1433 TGCGCA TGC^GCA
HgaI 1179 GCGTC GACGC(5/10)
KasI 938 GGCGCC G^GCGCC
Ksp632I 757 CTCTTC CTCTTC(1/4)
KspI 534 CCGCGG CCGC^GG
NarI 938 GGCGCC GG^CGCC
PflFI 163 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 785 CGATCG CGAT^CG
PvuII 1243 CAGCTG CAG^CTG
RcaI 518 TCATGA T^CATGA
SacII 534 CCGCGG CCGC^GG
SfoI 938 GGCGCC GGC^GCC
SmlI 241 CTTGAG C^T[CT][GA]AG
SspBI 962 TGTACA T^GTACA
Tth111I 163 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 629 CCAAGGCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1359 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 514 GACGTC G[GA]^CG[CT]C
938 GGCGCC
AfaI 963 GTAC GT^AC
1248 GTAC
ApaI 145 GGGCCC GGGCC^C
1377 GGGCCC
AvaI 65 CCCGAG C^[CT]CG[GA]G
478 CCCGAG
BfaI 55 CTAG C^TAG
920 CTAG
BglI 24 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1282 GCCATGAAGGC
BsaBI 546 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1032 GATCCCCATC
BsaHI 514 GACGTC G[GA]^CG[CT]C
938 GGCGCC
BsoBI 65 CCCGAG C^[CT]CG[GA]G
478 CCCGAG
BspMI 1325 ACCTGC ACCTGC(4/8)
234 GCAGGT
BstF5I 333 CATCC GGATG(2/0)
1038 CATCC
EcoRI 829 GAATTC G^AATTC
1339 GAATTC
EcoRV 436 GATATC GAT^ATC
1060 GATATC
FokI 333 CATCC GGATG(9/13)
1038 CATCC
Hin1I 514 GACGTC G[GA]^CG[CT]C
938 GGCGCC
MaeI 55 CTAG C^TAG
920 CTAG
MaeIII 85 GTCAC ^GT[ACGT]AC
115 GTCAC
MamI 546 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1032 GATCCCCATC
MspA1I 534 CCGCGG C[AC]G^C[GT]G
1243 CAGCTG
PleI 219 GACTC GAGTC(4/5)
1405 GAGTC
PpuMI 1322 AGGACCT [GA]G^G[AT]CC[CT]
1514 AGGACCT
PstI 1345 CTGCAG CTGCA^G
1510 CTGCAG
RsaI 963 GTAC GT^AC
1248 GTAC
StuI 22 AGGCCT AGG^CCT
896 AGGCCT
TfiI 489 GATTC G^A[AT]TC
707 GATTC
Tsp45I 85 GTCAC ^GT[GC]AC
115 GTCAC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BalI BanI BanII
BbvI BcnI BglII BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MflI MluNI
MnlI MscI MslI MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp509I TspRI TthHB8I XhoII