(Circular) (Six-base) MAPSORT of: pFN21AE6481.seq Check: 4841 from: 1 to: 6244 pFN21AE6481 6244 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2230 2230 Size: 6244 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2523 4054 278 Size: 53 83 186 936 987 1531 2468 Fragments arranged by size: 2468 1531 987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3482 4307 5691 5959 1632 Size: 1850 825 1384 268 1917 Fragments arranged by size: 1917 1850 1384 825 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 5143 6120 1203 Size: 3940 977 1327 Fragments arranged by size: 3940 1327 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 4973 4973 Size: 6244 AleI CACnn'nnGTG Cuts at: 1166 2115 1166 Size: 949 5295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AlwNI CAG_nnn'CTG Cuts at: 2052 3987 5559 2052 Size: 1935 1572 2737 Fragments arranged by size: 2737 1935 1572 ApaI G_GGCC'C Cuts at: 2154 3386 2154 Size: 1232 5012 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 2324 2834 3344 3553 3779 3931 6040 1493 Size: 831 510 510 209 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 510 510 226 209 152 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2483 3569 1870 Size: 73 127 413 1086 4545 Fragments arranged by size: 4545 1086 413 127 73 AvrII C'CTAG_G Cuts at: 2059 2059 Size: 6244 BamHI G'GATC_C Cuts at: 3023 3574 3941 3023 Size: 551 367 5326 Fragments arranged by size: 5326 551 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2258 2935 2943 3565 5020 Size: 325 217 627 471 677 8 622 1455 Cuts at: 5020 618 Size: 1842 Fragments arranged by size: 1842 1455 677 627 622 471 325 217 8 BanII G_rGCy'C Cuts at: 1391 2040 2154 2577 3386 3563 1391 Size: 649 114 423 809 177 4072 Fragments arranged by size: 4072 809 649 423 177 114 BbeI G_GCGC'C Cuts at: 2947 2947 Size: 6244 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2318 928 Size: 971 419 4854 Fragments arranged by size: 4854 971 419 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4435 4469 1903 Size: 34 2498 34 3678 Fragments arranged by size: 3678 2498 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 1548 Size: 6244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2035 3293 4855 Size: 107 122 71 631 605 363 1258 1562 Cuts at: 4855 136 Size: 1525 Fragments arranged by size: 1562 1525 1258 631 605 363 122 107 71 BglII A'GATC_T Cuts at: 2601 3114 3147 6239 2601 Size: 513 33 3092 2606 Fragments arranged by size: 3092 2606 513 33 BlpI GC'TnA_GC Cuts at: 3661 3661 Size: 6244 Bme1580I G_kGCm'C Cuts at: 1790 2154 3386 4305 5461 1790 Size: 364 1232 919 1156 2573 Fragments arranged by size: 2573 1232 1156 919 364 BmgBI CAC'GTC Cuts at: 1318 3280 1318 Size: 1962 4282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 3975 4933 Size: 416 53 83 399 24 762 1796 958 Cuts at: 4933 442 Size: 1753 Fragments arranged by size: 1796 1753 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3415 3415 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4904 1239 Size: 3665 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 4369 5234 5532 2267 Size: 2102 865 298 2979 Fragments arranged by size: 2979 2102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2920 4907 882 Size: 617 1421 1987 2219 Fragments arranged by size: 2219 1987 1421 617 BsaAI yAC'GTr Cuts at: 493 1204 3121 4035 493 Size: 711 1917 914 2702 Fragments arranged by size: 2702 1917 914 711 BsaBI GATnn'nnATC Cuts at: 2555 3041 6238 2555 Size: 486 3197 2561 Fragments arranged by size: 3197 2561 486 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2520 2944 4051 4433 Size: 53 83 186 936 987 424 1107 382 Cuts at: 4433 5068 275 Size: 635 1451 Fragments arranged by size: 1451 1107 987 936 635 424 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2350 4677 5075 5349 5496 5907 2350 Size: 2327 398 274 147 411 2687 Fragments arranged by size: 2687 2327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2453 2483 1178 Size: 30 165 30 1050 30 4939 Fragments arranged by size: 4939 1050 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2245 2501 2945 3323 1186 Size: 1059 256 444 378 4107 Fragments arranged by size: 4107 1059 444 378 256 BseYI C'CCAG_C Cuts at: 2368 5447 5943 2368 Size: 3079 496 2669 Fragments arranged by size: 3079 2669 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2085 2158 3302 1163 Size: 573 349 73 1144 4105 Fragments arranged by size: 4105 1144 573 349 73 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2793 3694 4455 5483 1540 Size: 275 186 792 901 761 1028 2301 Fragments arranged by size: 2301 1028 901 792 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3171 4305 4390 5461 3171 Size: 1134 85 1071 3954 Fragments arranged by size: 3954 1134 1071 85 BsmI GAATG_Cn' Cuts at: 3468 3755 3848 3468 Size: 287 93 5864 Fragments arranged by size: 5864 287 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2154 2577 3171 3386 3563 4305 Size: 399 250 114 423 594 215 177 742 Cuts at: 4305 4390 5461 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 594 423 399 250 215 177 114 85 BspEI T'CCGG_A Cuts at: 5907 5907 Size: 6244 BspHI T'CATG_A Cuts at: 2523 4133 5863 2523 Size: 1610 1730 2904 Fragments arranged by size: 2904 1730 1610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2230 3339 3599 844 Size: 1386 1109 260 3489 Fragments arranged by size: 3489 1386 1109 260 BsrBI CCG'CTC Cuts at: 1394 1823 2235 2597 2797 2995 3399 4131 1394 Size: 429 412 362 200 198 404 732 3507 Fragments arranged by size: 3507 732 429 412 404 362 200 198 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 4739 4913 65 Size: 1183 1284 2207 174 1396 Fragments arranged by size: 2207 1396 1284 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4888 5075 1700 Size: 110 3078 187 2869 Fragments arranged by size: 3078 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2967 96 Size: 2871 3373 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 2052 794 Size: 1258 4986 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 2089 1184 Size: 905 5339 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6244 BstYI r'GATC_y Cuts at: 1972 2550 2601 3023 3036 3114 3147 3574 3941 Size: 578 51 422 13 78 33 427 367 Cuts at: 3941 4326 4343 5795 5881 5893 6239 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 578 427 422 385 367 346 86 78 51 33 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2539 2685 2745 3229 513 Size: 552 939 6 529 146 60 484 3528 Fragments arranged by size: 3528 939 552 529 484 146 60 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5505 Fragments arranged by size: 5505 683 56 BtsI GCAGTG_nn' Cuts at: 2146 2826 3534 3760 4554 4574 2146 Size: 680 708 226 794 20 3816 Fragments arranged by size: 3816 794 708 680 226 20 ClaI AT'CG_AT Cuts at: 1554 2301 1554 Size: 747 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 3548 3548 Size: 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5251 809 Size: 507 3935 1802 Fragments arranged by size: 3935 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2449 2542 Size: 54 1425 50 275 18 177 442 93 Cuts at: 2542 2775 2979 3691 4580 6209 8 Size: 233 204 712 889 1629 43 Fragments arranged by size: 1629 1425 889 712 442 275 233 204 177 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2768 4174 1353 Size: 1415 1406 3423 Fragments arranged by size: 3423 1415 1406 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2441 4831 5205 5351 387 Size: 1064 138 852 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 852 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3482 4307 4904 5691 5959 1239 Size: 393 1850 825 597 787 268 1524 Fragments arranged by size: 1850 1524 825 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2151 3328 3382 3383 3520 2151 Size: 1177 54 1 137 4875 Fragments arranged by size: 4875 1177 137 54 1 EcoRI G'AATT_C Cuts at: 2834 3344 3553 3931 2834 Size: 510 209 378 5147 Fragments arranged by size: 5147 510 378 209 EcoRV GAT'ATC Cuts at: 2443 3067 2443 Size: 624 5620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2743 2743 Size: 6244 FspI TGC'GCA Cuts at: 3440 4750 3440 Size: 1310 4934 HaeII r_GCGC'y Cuts at: 1678 2822 2947 3317 3515 5391 1678 Size: 1144 125 370 198 1876 2531 Fragments arranged by size: 2531 1876 1144 370 198 125 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2581 2613 3012 3044 4885 Size: 32 135 32 1009 32 399 32 1841 Cuts at: 4885 4917 4959 4991 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1841 1009 399 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6244 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KasI G'GCGC_C Cuts at: 2943 2943 Size: 6244 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3267 5358 5542 1305 Size: 1962 2091 184 2007 Fragments arranged by size: 2091 2007 1962 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2451 2777 2981 10 Size: 54 1425 343 177 442 326 204 3273 Fragments arranged by size: 3273 1425 442 343 326 204 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2115 2690 3164 3234 4202 4561 Size: 648 843 106 575 474 70 968 359 Cuts at: 4561 4720 518 Size: 159 2042 Fragments arranged by size: 2042 968 843 648 575 474 359 159 106 70 MspA1I CmG'CkG Cuts at: 1606 2541 3250 3660 4337 5485 5730 1606 Size: 935 709 410 677 1148 245 2120 Fragments arranged by size: 2120 1148 935 709 677 410 245 NarI GG'CG_CC Cuts at: 2944 2944 Size: 6244 NcoI C'CATG_G Cuts at: 513 1065 2004 2010 2685 2745 3229 513 Size: 552 939 6 675 60 484 3528 Fragments arranged by size: 3528 939 675 552 484 60 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 2083 2368 2864 3602 5147 1132 Size: 97 854 285 496 738 1545 2229 Fragments arranged by size: 2229 1545 854 738 496 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6244 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6244 PfoI T'CCnGG_A Cuts at: 2956 2956 Size: 6244 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3328 3520 3328 Size: 192 6052 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6244 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PspOMI G'GGCC_C Cuts at: 2150 3382 2150 Size: 1232 5012 PstI C_TGCA'G Cuts at: 830 3354 3519 3596 830 Size: 2524 165 77 3478 Fragments arranged by size: 3478 2524 165 77 PvuI CG_AT'CG Cuts at: 2001 2793 2001 Size: 792 5452 PvuII CAG'CTG Cuts at: 3250 3250 Size: 6244 SacII CC_GC'GG Cuts at: 2542 2542 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 4492 6204 4492 Size: 1712 4532 SfcI C'TryA_G Cuts at: 826 1045 2125 3350 3515 3592 4727 5408 5599 Size: 219 1080 1225 165 77 1135 681 191 Cuts at: 5599 826 Size: 1471 Fragments arranged by size: 1471 1225 1135 1080 681 219 191 165 77 SfoI GGC'GCC Cuts at: 2945 2945 Size: 6244 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 3571 1872 Size: 1699 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 4348 5249 5511 820 Size: 197 926 303 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 303 262 197 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 4168 5 Size: 47 4116 2081 Fragments arranged by size: 4116 2081 47 StuI AGG'CCT Cuts at: 1517 2029 2903 1517 Size: 512 874 4858 Fragments arranged by size: 4858 874 512 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2059 2262 2634 2685 2745 Size: 552 939 6 49 203 372 51 60 Cuts at: 2745 3229 3450 3683 513 Size: 484 221 233 3074 Fragments arranged by size: 3074 939 552 484 372 233 221 203 60 51 49 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 3086 4271 4288 4441 4626 5071 Size: 1255 543 963 1185 17 153 185 445 Cuts at: 5071 325 Size: 1498 Fragments arranged by size: 1498 1255 1185 963 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2967 4490 6202 Size: 274 80 33 51 1443 990 1523 1712 Cuts at: 6202 96 Size: 138 Fragments arranged by size: 1712 1523 1443 990 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6204 6236 6204 Size: 32 6212 Tth111I GACn'n_nGTC Cuts at: 1316 2171 1316 Size: 855 5389 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 3569 1870 Size: 1699 4545 XmnI GAAnn'nnTTC Cuts at: 3368 4373 3368 Size: 1005 5239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2521 4052 276 Size: 53 83 186 936 987 1531 2468 Fragments arranged by size: 2468 1531 987 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI