(Linear) (Six-base) MAPSORT of: KIEE6481.seq Check: 3233 from: 1 to: 1539 KIEE6481.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 225 1539 Size: 225 1314 AatII G_ACGT'C Cuts at: 0 518 1539 Size: 518 1021 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1477 1539 Size: 1477 62 AleI CACnn'nnGTG Cuts at: 0 110 1539 Size: 110 1429 AlwNI CAG_nnn'CTG Cuts at: 0 47 1539 Size: 47 1492 ApaI G_GGCC'C Cuts at: 0 149 1381 1539 Size: 149 1232 158 Fragments arranged by size: 1232 158 149 ApoI r'AATT_y Cuts at: 0 319 829 1339 1539 Size: 319 510 510 200 Fragments arranged by size: 510 510 319 200 AvaI C'yCGr_G Cuts at: 0 65 478 1539 Size: 65 413 1061 Fragments arranged by size: 1061 413 65 AvrII C'CTAG_G Cuts at: 0 54 1539 Size: 54 1485 BamHI G'GATC_C Cuts at: 0 1018 1539 Size: 1018 521 BanI G'GyrC_C Cuts at: 0 253 930 938 1539 Size: 253 677 8 601 Fragments arranged by size: 677 601 253 8 BanII G_rGCy'C Cuts at: 0 35 149 572 1381 1539 Size: 35 114 423 809 158 Fragments arranged by size: 809 423 158 114 35 BbeI G_GCGC'C Cuts at: 0 942 1539 Size: 942 597 BbsI GAAGACnn'nnnn_ Cuts at: 0 313 1539 Size: 313 1226 BglI GCCn_nnn'nGGC Cuts at: 0 30 1288 1539 Size: 30 1258 251 Fragments arranged by size: 1258 251 30 BglII A'GATC_T Cuts at: 0 596 1109 1142 1539 Size: 596 513 33 397 Fragments arranged by size: 596 513 397 33 Bme1580I G_kGCm'C Cuts at: 0 149 1381 1539 Size: 149 1232 158 Fragments arranged by size: 1232 158 149 BmgBI CAC'GTC Cuts at: 0 1275 1539 Size: 1275 264 BmrI ACTGGGnnnn_n' Cuts at: 0 174 1539 Size: 174 1365 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1410 1539 Size: 1410 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 1539 Size: 262 1277 BsaI GGTCTCn'nnnn_ Cuts at: 0 915 1539 Size: 915 624 BsaAI yAC'GTr Cuts at: 0 1116 1539 Size: 1116 423 BsaBI GATnn'nnATC Cuts at: 0 550 1036 1539 Size: 550 486 503 Fragments arranged by size: 550 503 486 BsaHI Gr'CG_yC Cuts at: 0 515 939 1539 Size: 515 424 600 Fragments arranged by size: 600 515 424 BsaWI w'CCGG_w Cuts at: 0 345 1539 Size: 345 1194 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 448 478 1539 Size: 448 30 1061 Fragments arranged by size: 1061 448 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 240 496 940 1318 1539 Size: 240 256 444 378 221 Fragments arranged by size: 444 378 256 240 221 BseYI C'CCAG_C Cuts at: 0 363 1539 Size: 363 1176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 153 1297 1539 Size: 80 73 1144 242 Fragments arranged by size: 1144 242 80 73 BsiEI CG_ry'CG Cuts at: 0 788 1539 Size: 788 751 BsiHKAI G_wGCw'C Cuts at: 0 1166 1539 Size: 1166 373 BsmI GAATG_Cn' Cuts at: 0 1463 1539 Size: 1463 76 Bsp1286I G_dGCh'C Cuts at: 0 35 149 572 1166 1381 1539 Size: 35 114 423 594 215 158 Fragments arranged by size: 594 423 215 158 114 35 BspHI T'CATG_A Cuts at: 0 518 1539 Size: 518 1021 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 225 1334 1539 Size: 225 1109 205 Fragments arranged by size: 1109 225 205 BsrBI CCG'CTC Cuts at: 0 230 592 792 990 1394 1539 Size: 230 362 200 198 404 145 Fragments arranged by size: 404 362 230 200 198 145 BsrDI GCAATG_nn' Cuts at: 0 527 1539 Size: 527 1012 BsrGI T'GTAC_A Cuts at: 0 962 1539 Size: 962 577 BstAPI GCAn_nnn'nTGC Cuts at: 0 47 1539 Size: 47 1492 BstEII G'GTnAC_C Cuts at: 0 84 1539 Size: 84 1455 BstYI r'GATC_y Cuts at: 0 545 596 1018 1031 1109 1142 1539 Size: 545 51 422 13 78 33 397 Fragments arranged by size: 545 422 397 78 51 33 13 BtgI C'CryG_G Cuts at: 0 5 534 680 740 1224 1539 Size: 5 529 146 60 484 315 Fragments arranged by size: 529 484 315 146 60 5 BtsI GCAGTG_nn' Cuts at: 0 141 821 1529 1539 Size: 141 680 708 10 Fragments arranged by size: 708 680 141 10 ClaI AT'CG_AT Cuts at: 0 296 1539 Size: 296 1243 EaeI y'GGCC_r Cuts at: 0 2 444 537 770 974 1539 Size: 2 442 93 233 204 565 Fragments arranged by size: 565 442 233 204 93 2 EarI CTCTTCn'nnn_ Cuts at: 0 763 1539 Size: 763 776 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 436 1539 Size: 436 1103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1477 1539 Size: 1477 62 EcoO109I rG'GnC_Cy Cuts at: 0 146 1323 1377 1378 1515 1539 Size: 146 1177 54 1 137 24 Fragments arranged by size: 1177 146 137 54 24 1 EcoRI G'AATT_C Cuts at: 0 829 1339 1539 Size: 829 510 200 Fragments arranged by size: 829 510 200 EcoRV GAT'ATC Cuts at: 0 438 1062 1539 Size: 438 624 477 Fragments arranged by size: 624 477 438 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 738 1539 Size: 738 801 FspI TGC'GCA Cuts at: 0 1435 1539 Size: 1435 104 HaeII r_GCGC'y Cuts at: 0 817 942 1312 1510 1539 Size: 817 125 370 198 29 Fragments arranged by size: 817 370 198 125 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 576 608 1007 1039 1539 Size: 576 32 399 32 500 Fragments arranged by size: 576 500 399 32 32 KasI G'GCGC_C Cuts at: 0 938 1539 Size: 938 601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1262 1539 Size: 1262 277 MscI TGG'CCA Cuts at: 0 4 446 772 976 1539 Size: 4 442 326 204 563 Fragments arranged by size: 563 442 326 204 4 MslI CAynn'nnrTG Cuts at: 0 110 685 1159 1229 1539 Size: 110 575 474 70 310 Fragments arranged by size: 575 474 310 110 70 MspA1I CmG'CkG Cuts at: 0 536 1245 1539 Size: 536 709 294 Fragments arranged by size: 709 536 294 NarI GG'CG_CC Cuts at: 0 939 1539 Size: 939 600 NcoI C'CATG_G Cuts at: 0 5 680 740 1224 1539 Size: 5 675 60 484 315 Fragments arranged by size: 675 484 315 60 5 NspI r_CATG'y Cuts at: 0 78 363 859 1539 Size: 78 285 496 680 Fragments arranged by size: 680 496 285 78 PfoI T'CCnGG_A Cuts at: 0 951 1539 Size: 951 588 PpuMI rG'GwC_Cy Cuts at: 0 1323 1515 1539 Size: 1323 192 24 Fragments arranged by size: 1323 192 24 PspOMI G'GGCC_C Cuts at: 0 145 1377 1539 Size: 145 1232 162 Fragments arranged by size: 1232 162 145 PstI C_TGCA'G Cuts at: 0 1349 1514 1539 Size: 1349 165 25 Fragments arranged by size: 1349 165 25 PvuI CG_AT'CG Cuts at: 0 788 1539 Size: 788 751 PvuII CAG'CTG Cuts at: 0 1245 1539 Size: 1245 294 SacII CC_GC'GG Cuts at: 0 537 1539 Size: 537 1002 SfcI C'TryA_G Cuts at: 0 120 1345 1510 1539 Size: 120 1225 165 29 Fragments arranged by size: 1225 165 120 29 SfoI GGC'GCC Cuts at: 0 940 1539 Size: 940 599 SmlI C'TyrA_G Cuts at: 0 241 1539 Size: 241 1298 StuI AGG'CCT Cuts at: 0 24 898 1539 Size: 24 874 641 Fragments arranged by size: 874 641 24 StyI C'CwwG_G Cuts at: 0 5 54 257 629 680 740 1224 1445 Size: 5 49 203 372 51 60 484 221 Cuts at: 1445 1539 Size: 94 Fragments arranged by size: 484 372 221 203 94 60 51 49 5 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 118 1081 1539 Size: 118 963 458 Fragments arranged by size: 963 458 118 TatI w'GTAC_w Cuts at: 0 962 1539 Size: 962 577 Tth111I GACn'n_nGTC Cuts at: 0 166 1539 Size: 166 1373 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 636 1539 Size: 636 903 XmnI GAAnn'nnTTC Cuts at: 0 1363 1539 Size: 1363 176 ZraI GAC'GTC Cuts at: 0 516 1539 Size: 516 1023 Enzymes that do cut: AarI AatII AcuI AleI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstEII BstYI BtgI BtsI ClaI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NspI PfoI PpuMI PspOMI PstI PvuI PvuII SacII SfcI SfoI SmlI StuI StyI TaqII TatI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI BaeI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BpmI Bpu10I BsiWI BsmBI BspEI BsrFI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI XmaI