################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 534 GTAC GT^AC AflIII 233 ACATGT A^C[GA][CT]GT AvaII 86 GGACC G^G[AT]CC AvrII 381 CCTAGG C^CTAGG BalI 242 TGGCCA TGG^CCA BamHI 1213 GGATCC G^GATCC BanII 35 GGGCTC G[GA]GC[CT]^C BlnI 381 CCTAGG C^CTAGG BmyI 35 GGGCTC G[AGT]GC[ACT]^C BsaI 489 GGTCTC GGTCTC(1/5) BsmBI 103 CGTCTC CGTCTC(1/5) BsmFI 85 GGGAC GGGAC(10/14) Bsp1286I 35 GGGCTC G[AGT]GC[ACT]^C BspLU11I 233 ACATGT A^CATGT BssSI 177 CACGAG CACGAG(-5/-1) BstAPI 683 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 707 CCAAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 85 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 875 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1249 CCATGG C^C[GA][CT]GG EaeI 242 TGGCCA [CT]^GGCC[GA] EcoO109I 85 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1032 GATATC GAT^ATC FauI 346 GCGGG CCCGC(4/6) HincII 761 GTCAAC GT[CT]^[GA]AC HindII 761 GTCAAC GT[CT]^[GA]AC MaeII 102 ACGT A^CGT MfeI 1151 CAATTG C^AATTG MluNI 242 TGGCCA TGG^CCA MscI 242 TGGCCA TGG^CCA MspA1I 344 CAGCGG C[AC]G^C[GT]G MunI 1151 CAATTG C^AATTG NcoI 1249 CCATGG C^CATGG PpuMI 85 GGGACCT [GA]G^G[AT]CC[CT] PstI 1289 CTGCAG CTGCA^G RsaI 534 GTAC GT^AC SfcI 1289 CTGCAG C^T[GA][CT]AG SspI 801 AATATT AAT^ATT Tsp45I 1066 GTCAC ^GT[GC]AC XcmI 736 CCACTGATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 1037 CCGC CCGC(-3/-1) 346 GCGG AcsI 914 GAATTT [GA]^AATT[CT] 1091 AAATTC ApoI 914 GAATTT [GA]^AATT[CT] 1091 AAATTC BsrI 587 ACTGG ACTGG(1/-1) 1075 ACTGG BssKI 282 CCTGG ^CC[ACGT]GG 1053 CCTGG BstNI 282 CCTGG CC^[AT]GG 1053 CCTGG CfoI 189 GCGC GCG^C 695 GCGC EcoNI 76 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 852 CCTTAGTCAGG EcoRII 282 CCTGG ^CC[AT]GG 1053 CCTGG HhaI 189 GCGC GCG^C 695 GCGC HinP1I 189 GCGC G^CGC 695 GCGC HinfI 298 GAATC G^A[ACGT]TC 814 GATTC MaeIII 370 GTTAC ^GT[ACGT]AC 1066 GTCAC MvaI 282 CCTGG CC^[AT]GG 1053 CCTGG NspI 233 ACATGT [GA]CATG^[CT] 890 ACATGC ScrFI 282 CCTGG CC^[ACGT]GG 1053 CCTGG TfiI 298 GAATC G^A[AT]TC 814 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AluI AlwI AlwNI BbvI BfaI BsaJI BsgI BsiYI BslI BsmAI BstF5I BstYI Cac8I Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII ItaI MaeI MboI MboII MflI MnlI MseI MslI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I SfaNI StyI Tru9I TseI Tsp509I TspRI XhoII