(Circular) (Six-base) MAPSORT of: pFN21AE6463.seq Check: 1678 from: 1 to: 6205 pFN21AE6463 6205 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2892 2922 4268 5652 5920 1632 Size: 1260 30 1346 1384 268 1917 Fragments arranged by size: 1917 1384 1346 1260 268 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 2238 5104 6081 1203 Size: 1035 2866 977 1327 Fragments arranged by size: 2866 1327 1035 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 2366 2529 2743 3293 3948 5520 2366 Size: 163 214 550 655 1572 3051 Fragments arranged by size: 3051 1572 655 550 214 163 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 2919 3096 3514 3740 3892 6001 1493 Size: 1426 177 418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1426 418 226 177 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 3530 1870 Size: 73 1587 4545 Fragments arranged by size: 4545 1587 73 AvrII C'CTAG_G Cuts at: 2386 2386 Size: 6205 BamHI G'GATC_C Cuts at: 3218 3535 3902 3218 Size: 317 367 5521 Fragments arranged by size: 5521 367 317 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3526 4981 618 Size: 325 217 627 1739 1455 1842 Fragments arranged by size: 1842 1739 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2044 3524 1391 Size: 653 1480 4072 Fragments arranged by size: 4072 1480 653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4396 4430 1903 Size: 34 50 34 2375 34 3678 Fragments arranged by size: 3678 2375 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 3622 3622 Size: 6205 Bme1580I G_kGCm'C Cuts at: 1790 4266 5422 1790 Size: 2476 1156 2573 Fragments arranged by size: 2573 2476 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3090 3936 4894 Size: 416 53 83 399 24 1673 846 958 Cuts at: 4894 442 Size: 1753 Fragments arranged by size: 1753 1673 958 846 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2058 2058 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4865 1239 Size: 3626 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4330 5195 5493 4330 Size: 865 298 5042 Fragments arranged by size: 5042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2500 4868 882 Size: 617 1001 2368 2219 Fragments arranged by size: 2368 2219 1001 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4012 4394 5029 275 Size: 53 83 186 936 2479 382 635 1451 Fragments arranged by size: 2479 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5980 Fragments arranged by size: 5980 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6205 BseYI C'CCAG_C Cuts at: 2751 5408 5904 2751 Size: 2657 496 3052 Fragments arranged by size: 3052 2657 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2055 2553 2571 1163 Size: 573 319 498 18 4797 Fragments arranged by size: 4797 573 498 319 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4266 4351 5422 4266 Size: 85 1071 5049 Fragments arranged by size: 5049 1071 85 BsmI GAATG_Cn' Cuts at: 3716 3809 3716 Size: 93 6112 BsmBI CGTCTCn'nnnn_ Cuts at: 2114 2114 Size: 6205 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 3524 4266 4351 5422 1391 Size: 399 254 1480 742 85 1071 2174 Fragments arranged by size: 2174 1480 1071 742 399 254 85 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4700 4874 65 Size: 1183 3452 174 1396 Fragments arranged by size: 3452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4849 5036 1700 Size: 110 3039 187 2869 Fragments arranged by size: 3039 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 2182 4265 5277 2182 Size: 2083 1012 3110 Fragments arranged by size: 3110 2083 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2694 794 Size: 1900 4305 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 2719 2719 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2435 2875 3218 3535 3902 4287 4304 5756 Size: 463 440 343 317 367 385 17 1452 Cuts at: 5756 5842 5854 6200 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 463 440 385 367 346 343 317 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3254 513 Size: 552 2189 3464 Fragments arranged by size: 3464 2189 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 2067 3721 4515 4535 2067 Size: 1654 794 20 3737 Fragments arranged by size: 3737 1654 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DraIII CAC_nnn'GTG Cuts at: 2885 2885 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 3652 4541 Size: 54 1425 50 275 18 417 1405 889 Cuts at: 4541 6170 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1405 889 417 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4135 1353 Size: 2782 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2892 2922 4268 4865 5652 5920 1239 Size: 393 1260 30 1346 597 787 268 1524 Fragments arranged by size: 1524 1346 1260 787 597 393 268 30 EcoNI CCTnn'n_nnAGG Cuts at: 2085 2861 2085 Size: 776 5429 EcoO109I rG'GnC_Cy Cuts at: 2091 2091 Size: 6205 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 EcoRV GAT'ATC Cuts at: 3039 3039 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 5352 1678 Size: 3674 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4846 4878 4920 4952 1373 Size: 32 135 32 3274 32 42 32 2626 Fragments arranged by size: 3274 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2768 3549 3795 669 Size: 988 281 830 781 246 3079 Fragments arranged by size: 3079 988 830 781 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3156 3804 3156 Size: 648 5557 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5319 5503 1305 Size: 4014 184 2007 Fragments arranged by size: 4014 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 10 Size: 54 1425 343 417 3966 Fragments arranged by size: 3966 1425 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2506 3378 4163 4522 4681 518 Size: 648 1077 263 872 785 359 159 2042 Fragments arranged by size: 2042 1077 872 785 648 359 263 159 MspA1I CmG'CkG Cuts at: 1606 2351 3621 4298 5446 5691 1606 Size: 745 1270 677 1148 245 2120 Fragments arranged by size: 2120 1270 1148 745 677 245 NcoI C'CATG_G Cuts at: 513 1065 3254 513 Size: 552 2189 3464 Fragments arranged by size: 3464 2189 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2242 2899 3563 5108 1132 Size: 97 1013 657 664 1545 2229 Fragments arranged by size: 2229 1545 1013 664 657 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 2238 5104 2238 Size: 2866 3339 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6205 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2091 2091 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 2448 2678 3278 3775 2448 Size: 230 600 497 4878 Fragments arranged by size: 4878 600 497 230 PstI C_TGCA'G Cuts at: 830 3298 3557 830 Size: 2468 259 3478 Fragments arranged by size: 3478 2468 259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 3294 3553 4688 5369 5560 826 Size: 219 2249 259 1135 681 191 1471 Fragments arranged by size: 2249 1471 1135 681 259 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4309 5210 5472 820 Size: 197 926 2366 901 262 1553 Fragments arranged by size: 2366 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 2808 4129 5 Size: 47 2756 1321 2081 Fragments arranged by size: 2756 2081 1321 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2114 2386 2431 2455 3244 3254 3644 Size: 552 1049 272 45 24 789 10 390 Cuts at: 3644 513 Size: 3074 Fragments arranged by size: 3074 1049 789 552 390 272 45 24 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2150 4232 4249 4402 4587 5032 325 Size: 1255 570 2082 17 153 185 445 1498 Fragments arranged by size: 2082 1498 1255 570 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4451 6163 96 Size: 274 80 33 51 1443 2474 1712 138 Fragments arranged by size: 2474 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3210 3242 6165 6197 3210 Size: 32 2923 32 3218 Fragments arranged by size: 3218 2923 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2748 2748 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI