################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I FauI FbaI FseI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflIII 654 ACATGT A^C[GA][CT]GT Alw44I 449 GTGCAC G^TGCAC AlwI 759 GGATC GGATC(4/5) ApaLI 449 GTGCAC G^TGCAC Asp700I 304 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 449 GTGCAC G[AT]GC[AT]^C BciVI 686 GGATAC GTATCC(6/5) BpmI 1166 CTGGAG CTGGAG(16/14) BsaI 1527 GAGACC GGTCTC(1/5) BseRI 202 GAGGAG GAGGAG(10/8) BsiHKAI 449 GTGCAC G[AT]GC[AT]^C BspLU11I 654 ACATGT A^CATGT BstAPI 290 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 759 GGATCT [GA]^GATC[CT] Cac8I 271 GCCAGC GC[ACGT]^[ACGT]GC DraIII 143 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 184 GAATTC G^AATTC EcoT22I 297 ATGCAT ATGCA^T HgaI 1066 GCGTC GACGC(5/10) HindIII 1468 AAGCTT A^AGCTT HpaI 720 GTTAAC GTT^AAC HphI 171 TCACC GGTGA(8/7) MaeII 1478 ACGT A^CGT MfeI 1107 CAATTG C^AATTG MflI 759 GGATCT [GA]^GATC[CT] MslI 286 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1107 CAATTG C^AATTG NsiI 297 ATGCAT ATGCA^T PflMI 1317 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 307 GCTCTTC GCTCTTC(1/4) SfcI 1187 CTACAG C^T[GA][CT]AG SmlI 1252 CTTGAG C^T[CT][GA]AG Van91I 1317 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 238 CCAGAACTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 759 GGATCT [GA]^GATC[CT] XmnI 304 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 403 GTATAC GT^[AC][GT]AC 481 GTATAC AciI 1334 CCGC CCGC(-3/-1) 1445 CCGC AfaI 890 GTAC GT^AC 1073 GTAC AlwNI 601 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 1337 CAGCGACTG BanII 797 GAGCCC G[GA]GC[CT]^C 1235 GAGCCC BsrDI 824 CATTGC GCAATG(2/0) 854 CATTGC BssKI 135 CCAGG ^CC[ACGT]GG 1058 CCAGG Bst1107I 403 GTATAC GTA^TAC 481 GTATAC BstNI 135 CCAGG CC^[AT]GG 1058 CCAGG BstZ17I 403 GTATAC GTA^TAC 481 GTATAC DpnII 760 GATC ^GATC 1207 GATC DsaI 431 CCATGG C^C[GA][CT]GG 1317 CCATGG EcoRII 135 CCAGG ^CC[AT]GG 1058 CCAGG EcoRV 641 GATATC GAT^ATC 1029 GATATC EcoT14I 431 CCATGG C^C[AT][AT]GG 1317 CCATGG MboI 760 GATC ^GATC 1207 GATC MvaI 135 CCAGG CC^[AT]GG 1058 CCAGG NcoI 431 CCATGG C^CATGG 1317 CCATGG NdeII 760 GATC ^GATC 1207 GATC RsaI 890 GTAC GT^AC 1073 GTAC Sau3AI 760 GATC ^GATC 1207 GATC ScrFI 135 CCAGG CC^[ACGT]GG 1058 CCAGG StyI 431 CCATGG C^C[AT][AT]GG 1317 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AluI ApoI AvaII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BstF5I Cfr13I DdeI EarI Fnu4HI FokI HincII HindII HinfI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MwoI NlaIII NlaIV NspI Sau96I SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I