Restriction map for
pFN21AE6445
(Circular) (Six-base) MAPSORT of: pFN21AE6445.seq Check: 6125 from: 1 to: 6268
pFN21AE6445 6268 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2409 2487 3611 1937
Size: 472 78 1124 4594
Fragments arranged by size:
4594 1124 472 78
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1998 2588 2688 3060 4331 5715 5983 1632
Size: 366 590 100 372 1271 1384 268 1917
Fragments arranged by size:
1917 1384 1271 590 372 366 268 100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 2659 5167 6144 1203
Size: 1456 2508 977 1327
Fragments arranged by size:
2508 1456 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2303 1184
Size: 1119 5149
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3487 3519 3487
Size: 32 6236
AlwNI CAG_nnn'CTG
Cuts at: 2611 3347 4011 5583 2611
Size: 736 664 1572 3296
Fragments arranged by size:
3296 1572 736 664
ApaLI G'TGCA_C
Cuts at: 2454 4325 5481 2454
Size: 1871 1156 3241
Fragments arranged by size:
3241 1871 1156
ApoI r'AATT_y
Cuts at: 1493 2092 2189 2619 3577 3803 3955 6064 1493
Size: 599 97 430 958 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 599 430 226 152 97
AseI AT'TA_AT
Cuts at: 160 1032 4822 160
Size: 872 3790 1606
Fragments arranged by size:
3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 3593 1870
Size: 73 1650 4545
Fragments arranged by size:
4545 1650 73
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3589 5044 618
Size: 325 217 627 1802 1455 1842
Fragments arranged by size:
1842 1802 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2806 3244 3587 1391
Size: 1415 438 343 4072
Fragments arranged by size:
4072 1415 438 343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4459 4493 1903
Size: 34 2522 34 3678
Fragments arranged by size:
3678 2522 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2685 4160 5370 2685
Size: 1475 1210 3583
Fragments arranged by size:
3583 1475 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BfrBI ATG'CAT
Cuts at: 2304 2304
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4879 136
Size: 107 122 71 631 605 3207 1525
Fragments arranged by size:
3207 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 3685 3685
Size: 6268
Bme1580I G_kGCm'C
Cuts at: 1790 2458 4329 5485 1790
Size: 668 1871 1156 2573
Fragments arranged by size:
2573 1871 1156 668
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 3999 4957
Size: 416 53 83 399 24 1169 1413 958
Cuts at: 4957 442
Size: 1753
Fragments arranged by size:
1753 1413 1169 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3192 4928 1239
Size: 1953 1736 2579
Fragments arranged by size:
2579 1953 1736
Bpu10I CC'TnA_GC
Cuts at: 3309 3309
Size: 6268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3278 4393 5258 5556 3278
Size: 1115 865 298 3990
Fragments arranged by size:
3990 1115 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3526 4931 882
Size: 617 2027 1405 2219
Fragments arranged by size:
2219 2027 1405 617
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 6262 6262
Size: 6268
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4075 4457 5092 275
Size: 53 83 186 936 2542 382 635 1451
Fragments arranged by size:
2542 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4701 5099 5373 5520 5931 4701
Size: 398 274 147 411 5038
Fragments arranged by size:
5038 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 1178
Size: 30 165 30 621 30 5392
Fragments arranged by size:
5392 621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 1186
Size: 1036 5232
BseYI C'CCAG_C
Cuts at: 2463 2805 3425 5471 5967 2463
Size: 342 620 2046 496 2764
Fragments arranged by size:
2764 2046 620 496 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5695
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2458 4329 4414 5485 2458
Size: 1871 85 1071 3241
Fragments arranged by size:
3241 1871 1071 85
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2458 2806 3244 3587 4329 4414 5485
Size: 399 668 348 438 343 742 85 1071
Cuts at: 5485 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 668 438 399 348 343
85
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 4157 5887 4157
Size: 1730 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3623 844
Size: 2779 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2828 2858 4763 4937 65
Size: 1183 1580 30 1905 174 1396
Fragments arranged by size:
1905 1580 1396 1183 174 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 4912 5099 1700
Size: 110 3102 187 2869
Fragments arranged by size:
3102 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2301 794
Size: 1507 4761
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 2764 3598 3965 4350 4367 5819 5905 5917
Size: 792 834 367 385 17 1452 86 12
Cuts at: 5917 6263 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 834 792 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2410 2488 2410
Size: 78 6190
BtgI C'CryG_G
Cuts at: 513 1065 2436 3322 513
Size: 552 1371 886 3459
Fragments arranged by size:
3459 1371 886 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 2206 2302 3784 4578 4598 2206
Size: 96 1482 794 20 3876
Fragments arranged by size:
3876 1482 794 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DraIII CAC_nnn'GTG
Cuts at: 2153 2153
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3715 4604 6233
Size: 54 1425 50 275 18 1885 889 1629
Cuts at: 6233 8
Size: 43
Fragments arranged by size:
1885 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2607 3269 4198 1353
Size: 966 288 662 929 3423
Fragments arranged by size:
3423 966 929 662 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4855 5229 5375 387
Size: 1064 138 3266 374 146 1280
Fragments arranged by size:
3266 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2588 2688 3060 3192 4331 4928
Size: 393 366 590 100 372 132 1139 597
Cuts at: 4928 5715 5983 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1139 787 597 590 393 372 366
268 132 100
EcoRI G'AATT_C
Cuts at: 2189 3577 3955 2189
Size: 1388 378 4502
Fragments arranged by size:
4502 1388 378
EcoRV GAT'ATC
Cuts at: 2648 3036 2648
Size: 388 5880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2333 3491 2333
Size: 1158 5110
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 5415 1678
Size: 3737 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4909 4941 4983 5015 1373
Size: 32 135 32 3337 32 42 32 2626
Fragments arranged by size:
3337 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 2547 2727 3046 3612 3858
Size: 988 281 409 200 180 319 566 246
Cuts at: 3858 669
Size: 3079
Fragments arranged by size:
3079 988 566 409 319 281 246 200
180
HindIII A'AGCT_T
Cuts at: 748 3473 748
Size: 2725 3543
HpaI GTT'AAC
Cuts at: 2727 3858 2727
Size: 1131 5137
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 3112 3867 3112
Size: 755 5513
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5382 5566 1305
Size: 4077 184 2007
Fragments arranged by size:
4077 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4446
Fragments arranged by size:
4446 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2295 4226 4585 4744 518
Size: 648 1129 1931 359 159 2042
Fragments arranged by size:
2042 1931 1129 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3684 4361 5509 5754 1606
Size: 2078 677 1148 245 2120
Fragments arranged by size:
2120 2078 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2436 3322 513
Size: 552 1371 886 3459
Fragments arranged by size:
3459 1371 886 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NsiI A_TGCA'T
Cuts at: 2306 2306
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 2590 2663 2965 3626 5171 1132
Size: 97 1361 73 302 661 1545 2229
Fragments arranged by size:
2229 1545 1361 661 302 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6268
PciI A'CATG_T
Cuts at: 2659 5167 2659
Size: 2508 3760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3328 1108
Size: 2220 4048
PfoI T'CCnGG_A
Cuts at: 2139 2139
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 2751 3838 2751
Size: 1087 5181
PstI C_TGCA'G
Cuts at: 830 3620 830
Size: 2790 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SapI GCTCTTCn'nnn_
Cuts at: 2319 2319
Size: 6268
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 3192 3616 4751 5432 5623 826
Size: 219 2147 424 1135 681 191 1471
Fragments arranged by size:
2147 1471 1135 681 424 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3257 4372 5273 5535 820
Size: 197 926 1314 1115 901 262 1553
Fragments arranged by size:
1553 1314 1115 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3626 1132
Size: 2494 3774
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2436 3322 3707 513
Size: 552 1371 886 385 3074
Fragments arranged by size:
3074 1371 886 552 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4295 4312 4465 4650 5095 325
Size: 1255 2715 17 153 185 445 1498
Fragments arranged by size:
2715 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4514 6226 96
Size: 274 80 33 51 1443 2537 1712 138
Fragments arranged by size:
2537 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 6228 6260 2700
Size: 32 3496 32 2708
Fragments arranged by size:
3496 2708 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6268
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2250 2250
Size: 6268
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 2313 4397 2313
Size: 2084 4184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI