(Linear) (Six-base) MAPSORT of: KIEE6445.seq Check: 394 from: 1 to: 1563 KIEE6445.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 404 482 1563 Size: 404 78 1081 Fragments arranged by size: 1081 404 78 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 683 1055 1563 Size: 583 100 372 508 Fragments arranged by size: 583 508 372 100 AflIII A'CryG_T Cuts at: 0 654 1563 Size: 654 909 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 298 1563 Size: 298 1265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1482 1514 1563 Size: 1482 32 49 Fragments arranged by size: 1482 49 32 AlwNI CAG_nnn'CTG Cuts at: 0 606 1342 1563 Size: 606 736 221 Fragments arranged by size: 736 606 221 ApaLI G'TGCA_C Cuts at: 0 449 1563 Size: 449 1114 ApoI r'AATT_y Cuts at: 0 87 184 614 1563 Size: 87 97 430 949 Fragments arranged by size: 949 430 97 87 BanII G_rGCy'C Cuts at: 0 801 1239 1563 Size: 801 438 324 Fragments arranged by size: 801 438 324 BciVI GTATCCnnnnn_n' Cuts at: 0 680 1563 Size: 680 883 BfrBI ATG'CAT Cuts at: 0 299 1563 Size: 299 1264 Bme1580I G_kGCm'C Cuts at: 0 453 1563 Size: 453 1110 BmrI ACTGGGnnnn_n' Cuts at: 0 581 1563 Size: 581 982 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1278 1563 Size: 1278 285 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1187 1563 Size: 1187 376 Bpu10I CC'TnA_GC Cuts at: 0 1304 1563 Size: 1304 259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1273 1563 Size: 1273 290 BsaI GGTCTCn'nnnn_ Cuts at: 0 1521 1563 Size: 1521 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 19 49 1563 Size: 19 30 1514 Fragments arranged by size: 1514 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 217 1563 Size: 217 1346 BseYI C'CCAG_C Cuts at: 0 458 800 1420 1563 Size: 458 342 620 143 Fragments arranged by size: 620 458 342 143 BsiHKAI G_wGCw'C Cuts at: 0 453 1563 Size: 453 1110 Bsp1286I G_dGCh'C Cuts at: 0 453 801 1239 1563 Size: 453 348 438 324 Fragments arranged by size: 453 438 348 324 BsrDI GCAATG_nn' Cuts at: 0 823 853 1563 Size: 823 30 710 Fragments arranged by size: 823 710 30 BstAPI GCAn_nnn'nTGC Cuts at: 0 296 1563 Size: 296 1267 BstYI r'GATC_y Cuts at: 0 759 1563 Size: 759 804 BstZ17I GTA'TAC Cuts at: 0 405 483 1563 Size: 405 78 1080 Fragments arranged by size: 1080 405 78 BtgI C'CryG_G Cuts at: 0 431 1317 1563 Size: 431 886 246 Fragments arranged by size: 886 431 246 BtsI GCAGTG_nn' Cuts at: 0 201 297 1563 Size: 201 96 1266 Fragments arranged by size: 1266 201 96 DraIII CAC_nnn'GTG Cuts at: 0 148 1563 Size: 148 1415 EarI CTCTTCn'nnn_ Cuts at: 0 314 602 1264 1563 Size: 314 288 662 299 Fragments arranged by size: 662 314 299 288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 683 1055 1187 1563 Size: 583 100 372 132 376 Fragments arranged by size: 583 376 372 132 100 EcoRI G'AATT_C Cuts at: 0 184 1563 Size: 184 1379 EcoRV GAT'ATC Cuts at: 0 643 1031 1563 Size: 643 388 532 Fragments arranged by size: 643 532 388 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 328 1486 1563 Size: 328 1158 77 Fragments arranged by size: 1158 328 77 HincII GTy'rAC Cuts at: 0 342 542 722 1041 1563 Size: 342 200 180 319 522 Fragments arranged by size: 522 342 319 200 180 HindIII A'AGCT_T Cuts at: 0 1468 1563 Size: 1468 95 HpaI GTT'AAC Cuts at: 0 722 1563 Size: 722 841 MfeI C'AATT_G Cuts at: 0 1107 1563 Size: 1107 456 MslI CAynn'nnrTG Cuts at: 0 290 1563 Size: 290 1273 NcoI C'CATG_G Cuts at: 0 431 1317 1563 Size: 431 886 246 Fragments arranged by size: 886 431 246 NsiI A_TGCA'T Cuts at: 0 301 1563 Size: 301 1262 NspI r_CATG'y Cuts at: 0 585 658 960 1563 Size: 585 73 302 603 Fragments arranged by size: 603 585 302 73 PciI A'CATG_T Cuts at: 0 654 1563 Size: 654 909 PflMI CCAn_nnn'nTGG Cuts at: 0 1323 1563 Size: 1323 240 PfoI T'CCnGG_A Cuts at: 0 134 1563 Size: 134 1429 PsiI TTA'TAA Cuts at: 0 746 1563 Size: 746 817 SapI GCTCTTCn'nnn_ Cuts at: 0 314 1563 Size: 314 1249 SfcI C'TryA_G Cuts at: 0 1187 1563 Size: 1187 376 SmlI C'TyrA_G Cuts at: 0 1252 1563 Size: 1252 311 StyI C'CwwG_G Cuts at: 0 431 1317 1563 Size: 431 886 246 Fragments arranged by size: 886 431 246 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 695 727 1563 Size: 695 32 836 Fragments arranged by size: 836 695 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 245 1563 Size: 245 1318 XmnI GAAnn'nnTTC Cuts at: 0 308 1563 Size: 308 1255 Enzymes that do cut: AccI AcuI AflIII AlfI AloI AlwNI ApaLI ApoI BanII BciVI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrDI BstAPI BstYI BstZ17I BtgI BtsI DraIII EarI Eco57MI EcoRI EcoRV FalI HincII HindIII HpaI MfeI MslI NcoI NsiI NspI PciI PflMI PfoI PsiI SapI SfcI SmlI StyI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I KasI KpnI MluI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI ZraI