################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII HapII HgaI Hin1I HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 392 GTCTAC GT^[AC][GT]AC AciI 572 GCGG CCGC(-3/-1) AlwI 196 GATCC GGATC(4/5) AocI 604 CCTGAGG CC^T[ACGT]AGG AspHI 373 GTGCTC G[AT]GC[AT]^C BanI 402 GGCACC G^G[CT][GA]CC BbrPI 639 CACGTG CAC^GTG BbsI 90 GAAGAC GAAGAC(2/6) BbvI 1077 GCTGC GCAGC(8/12) BglII 233 AGATCT A^GATCT BpmI 397 CTCCAG CTGGAG(16/14) BpuAI 90 GAAGAC GAAGAC(2/6) BsaAI 639 CACGTG [CT]AC^GT[GA] BsgI 636 CTGCAC GTGCAG(16/14) BsiHKAI 373 GTGCTC G[AT]GC[AT]^C BspHI 743 TCATGA T^CATGA BspMI 634 ACCTGC ACCTGC(4/8) BsrDI 599 CATTGC GCAATG(2/0) BstEII 270 GGTGACC G^GT[ACGT]ACC BstPI 270 GGTGACC G^GT[ACGT]ACC BstXI 260 CCATAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 604 CCTGAGG CC^T[ACGT]AGG CfoI 610 GCGC GCG^C Eco81I 604 CCTGAGG CC^T[ACGT]AGG EcoO65I 270 GGTGACC G^GT[ACGT]ACC EcoT14I 1172 CCAAGG C^C[AT][AT]GG EcoT22I 409 ATGCAT ATGCA^T FauI 572 GCGGG CCCGC(4/6) Fnu4HI 1077 GCTGC GC^[ACGT]GC HhaI 610 GCGC GCG^C HinP1I 610 GCGC G^CGC HincII 825 GTCAAC GT[CT]^[GA]AC HindII 825 GTCAAC GT[CT]^[GA]AC ItaI 1077 GCTGC GC^[ACGT]GC MaeII 640 ACGT A^CGT MfeI 857 CAATTG C^AATTG MunI 857 CAATTG C^AATTG NsiI 409 ATGCAT ATGCA^T PleI 338 GACTC GAGTC(4/5) PmaCI 639 CACGTG CAC^GTG PmlI 639 CACGTG CAC^GTG RcaI 743 TCATGA T^CATGA SfcI 44 CTATAG C^T[GA][CT]AG StyI 1172 CCAAGG C^C[AT][AT]GG TseI 1077 GCTGC G^C[AT]GC TspRI 968 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 950 GAATTT [GA]^AATT[CT] 1302 AAATTT ApoI 950 GAATTT [GA]^AATT[CT] 1302 AAATTT BalI 224 TGGCCA TGG^CCA 701 TGGCCA BclI 740 TGATCA T^GATCA 1238 TGATCA BmyI 373 GTGCTC G[AGT]GC[ACT]^C 642 GTGCCC BsaI 516 GAGACC GGTCTC(1/5) 1018 GGTCTC BsaJI 645 CCCAGG C^C[ACGT][ACGT]GG 1172 CCAAGG Bsp1286I 373 GTGCTC G[AGT]GC[ACT]^C 642 GTGCCC BsrI 526 CCAGT ACTGG(1/-1) 850 CCAGT BssKI 399 CCAGG ^CC[ACGT]GG 646 CCAGG BstNI 399 CCAGG CC^[AT]GG 646 CCAGG BstYI 195 AGATCC [GA]^GATC[CT] 233 AGATCT Cac8I 612 GCAAGC GC[ACGT]^[ACGT]GC 895 GCAAGC EaeI 224 TGGCCA [CT]^GGCC[GA] 701 TGGCCA EarI 1082 CTCTTC CTCTTC(1/4) 513 GAAGAG EcoRII 399 CCAGG ^CC[AT]GG 646 CCAGG FbaI 740 TGATCA T^GATCA 1238 TGATCA HindIII 614 AAGCTT A^AGCTT 897 AAGCTT Ksp632I 1082 CTCTTC CTCTTC(1/4) 513 GAAGAG MflI 195 AGATCC [GA]^GATC[CT] 233 AGATCT MluNI 224 TGGCCA TGG^CCA 701 TGGCCA MscI 224 TGGCCA TGG^CCA 701 TGGCCA MspA1I 493 CAGCTG C[AC]G^C[GT]G 1153 CAGCTG MvaI 399 CCAGG CC^[AT]GG 646 CCAGG NlaIII 744 CATG CATG^ 1212 CATG NlaIV 402 GGCACC GG[ACGT]^[ACGT]CC 1194 GGCTCC PvuII 493 CAGCTG CAG^CTG 1153 CAGCTG ScrFI 399 CCAGG CC^[ACGT]GG 646 CCAGG TfiI 316 GATTC G^A[AT]TC 1008 GATTC XbaI 681 TCTAGA T^CTAGA 999 TCTAGA XhoII 195 AGATCC [GA]^GATC[CT] 233 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI AluI BfaI BsiYI BslI BsmAI BstF5I DdeI DpnII FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII RsaI Sau3AI SfaNI Tru9I Tsp45I Tsp509I