Restriction map for
pFN21AE6396
(Circular) (Six-base) MAPSORT of: pFN21AE6396.seq Check: 3413 from: 1 to: 6019
pFN21AE6396 6019 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3829 278
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2398 3362 1937
Size: 461 964 4594
Fragments arranged by size:
4594 964 461
Acc65I G'GTAC_C
Cuts at: 3340 3340
Size: 6019
AclI AA'CG_TT
Cuts at: 1563 4146 4519 1563
Size: 2583 373 3063
Fragments arranged by size:
3063 2583 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 2346 4082 5466 5734 1632
Size: 513 201 1736 1384 268 1917
Fragments arranged by size:
1917 1736 1384 513 268 201
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5822
AflIII A'CryG_T
Cuts at: 1203 4918 5895 1203
Size: 3715 977 1327
Fragments arranged by size:
3715 1327 977
AgeI A'CCGG_T
Cuts at: 4850 4850
Size: 6019
AhdI GACnn_n'nnGTC
Cuts at: 4748 4748
Size: 6019
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6019
AlwNI CAG_nnn'CTG
Cuts at: 3762 5334 3762
Size: 1572 4447
ApaLI G'TGCA_C
Cuts at: 4076 5232 4076
Size: 1156 4863
ApoI r'AATT_y
Cuts at: 1493 2955 3307 3328 3554 3706 5815 1493
Size: 1462 352 21 226 152 2109 1697
Fragments arranged by size:
2109 1697 1462 352 226 152 21
AseI AT'TA_AT
Cuts at: 160 1032 4573 160
Size: 872 3541 1606
Fragments arranged by size:
3541 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
AvaI C'yCGr_G
Cuts at: 1870 1943 3344 1870
Size: 73 1401 4545
Fragments arranged by size:
4545 1401 73
BamHI G'GATC_C
Cuts at: 3349 3716 3349
Size: 367 5652
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 3340 4795 618
Size: 325 217 627 620 933 1455 1842
Fragments arranged by size:
1842 1455 933 627 620 325 217
BanII G_rGCy'C
Cuts at: 1391 3338 1391
Size: 1947 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2102 928
Size: 971 203 4845
Fragments arranged by size:
4845 971 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4210 4244 1903
Size: 34 2273 34 3678
Fragments arranged by size:
3678 2273 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3911 5121 3911
Size: 1210 4809
BclI T'GATC_A
Cuts at: 1548 2745 3243 1548
Size: 1197 498 4324
Fragments arranged by size:
4324 1197 498
BfrBI ATG'CAT
Cuts at: 2416 2416
Size: 6019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4630 136
Size: 107 122 71 631 605 2958 1525
Fragments arranged by size:
2958 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2238 6014 2238
Size: 3776 2243
BlpI GC'TnA_GC
Cuts at: 3436 3436
Size: 6019
Bme1580I G_kGCm'C
Cuts at: 1790 2651 4080 5236 1790
Size: 861 1429 1156 2573
Fragments arranged by size:
2573 1429 1156 861
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3750 4708 442
Size: 416 53 83 399 24 2333 958 1753
Fragments arranged by size:
2333 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 4679 1239
Size: 1148 2292 2579
Fragments arranged by size:
2579 2292 1148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4144 5009 5307 4144
Size: 865 298 4856
Fragments arranged by size:
4856 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2515 3029 4682 882
Size: 617 1016 514 1653 2219
Fragments arranged by size:
2219 1653 1016 617 514
BsaAI yAC'GTr
Cuts at: 493 1204 2646 3810 493
Size: 711 1442 1164 2702
Fragments arranged by size:
2702 1442 1164 711
BsaBI GATnn'nnATC
Cuts at: 6013 6013
Size: 6019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3826 4208 4843 275
Size: 53 83 186 936 2293 382 635 1451
Fragments arranged by size:
2293 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4452 4850 5124 5271 5682 4452
Size: 398 274 147 411 4789
Fragments arranged by size:
4789 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5794
Fragments arranged by size:
5794 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6019
BseYI C'CCAG_C
Cuts at: 5222 5718 5222
Size: 496 5523
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2626 1163
Size: 573 890 4556
Fragments arranged by size:
4556 890 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3469 4230 5258 1540
Size: 275 186 1468 761 1028 2301
Fragments arranged by size:
2301 1468 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 4080 4165 5236 2382
Size: 1698 85 1071 3165
Fragments arranged by size:
3165 1698 1071 85
BsmI GAATG_Cn'
Cuts at: 3530 3623 3530
Size: 93 5926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2651 3338 4080 4165 5236 1391
Size: 399 592 269 687 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 592 399 269 85
BspEI T'CCGG_A
Cuts at: 5682 5682
Size: 6019
BspHI T'CATG_A
Cuts at: 2748 3908 5638 2748
Size: 1160 1730 3129
Fragments arranged by size:
3129 1730 1160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2648 3374 844
Size: 1804 726 3489
Fragments arranged by size:
3489 1804 726
BsrBI CCG'CTC
Cuts at: 1394 1823 3906 1394
Size: 429 2083 3507
Fragments arranged by size:
3507 2083 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2603 4514 4688 65
Size: 1183 1355 1911 174 1396
Fragments arranged by size:
1911 1396 1355 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4663 4850 1700
Size: 110 2853 187 2869
Fragments arranged by size:
2869 2853 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6019
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6019
BssSI C'ACGA_G
Cuts at: 4079 5091 4079
Size: 1012 5007
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6019
BstBI TT'CG_AA
Cuts at: 4823 4823
Size: 6019
BstEII G'GTnAC_C
Cuts at: 1184 2275 1184
Size: 1091 4928
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2272
Size: 6019
BstYI r'GATC_y
Cuts at: 1972 2200 2238 3349 3716 4101 4118 5570 5656
Size: 228 38 1111 367 385 17 1452 86
Cuts at: 5656 5668 6014 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1111 385 367 346 228 86
38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2610 2610
Size: 6019
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2055 487
Size: 683 56 829 4451
Fragments arranged by size:
4451 829 683 56
BtsI GCAGTG_nn'
Cuts at: 3535 4329 4349 3535
Size: 794 20 5205
Fragments arranged by size:
5205 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5984
DraI TTT'AAA
Cuts at: 3323 3323
Size: 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5026 809
Size: 507 3710 1802
Fragments arranged by size:
3710 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2706 3466
Size: 54 1425 50 275 18 399 477 760
Cuts at: 3466 4355 5984 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 760 477 399 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3466 1537
Size: 275 1654 4090
Fragments arranged by size:
4090 1654 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3093 3949 1353
Size: 1160 580 856 3423
Fragments arranged by size:
3423 1160 856 580
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4606 4980 5126 387
Size: 1064 138 3017 374 146 1280
Fragments arranged by size:
3017 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2346 2387 4082 4679 5466 5734
Size: 393 513 201 41 1695 597 787 268
Cuts at: 5734 1239
Size: 1524
Fragments arranged by size:
1695 1524 787 597 513 393 268 201
41
EcoRI G'AATT_C
Cuts at: 3328 3706 3328
Size: 378 5641
FspI TGC'GCA
Cuts at: 4525 4525
Size: 6019
HaeII r_GCGC'y
Cuts at: 1678 5166 1678
Size: 3488 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4660 4692 4734 4766 1373
Size: 32 135 32 3088 32 42 32 2626
Fragments arranged by size:
3088 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3363 3609 669
Size: 988 281 894 531 246 3079
Fragments arranged by size:
3079 988 894 531 281 246
HindIII A'AGCT_T
Cuts at: 748 2619 2902 748
Size: 1871 283 3865
Fragments arranged by size:
3865 1871 283
HpaI GTT'AAC
Cuts at: 3609 3609
Size: 6019
KpnI G_GTAC'C
Cuts at: 3344 3344
Size: 6019
MfeI C'AATT_G
Cuts at: 2862 3618 2862
Size: 756 5263
MluI A'CGCG_T
Cuts at: 5895 5895
Size: 6019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5133 5317 1305
Size: 3828 184 2007
Fragments arranged by size:
3828 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 2708 10
Size: 54 1425 343 399 477 3321
Fragments arranged by size:
3321 1425 477 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2714 2849 3977 4336 4495 518
Size: 648 1269 279 135 1128 359 159 2042
Fragments arranged by size:
2042 1269 1128 648 359 279 159 135
MspA1I CmG'CkG
Cuts at: 1606 2500 3160 3435 4112 5260 5505 1606
Size: 894 660 275 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 660 275 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5467
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6019
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6019
NotI GC'GGCC_GC
Cuts at: 3466 3466
Size: 6019
NsiI A_TGCA'T
Cuts at: 2418 2418
Size: 6019
NspI r_CATG'y
Cuts at: 1132 1229 3377 4922 1132
Size: 97 2148 1545 2229
Fragments arranged by size:
2229 2148 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6019
PciI A'CATG_T
Cuts at: 4918 4918
Size: 6019
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6019
PmeI GTTT'AAAC
Cuts at: 3323 3323
Size: 6019
PmlI CAC'GTG
Cuts at: 2646 2646
Size: 6019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 5601 5633 4088
Size: 32 1481 32 4474
Fragments arranged by size:
4474 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6019
PsiI TTA'TAA
Cuts at: 3589 3589
Size: 6019
PstI C_TGCA'G
Cuts at: 830 3371 830
Size: 2541 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6019
PvuII CAG'CTG
Cuts at: 2500 3160 2500
Size: 660 5359
SalI G'TCGA_C
Cuts at: 1936 3361 1936
Size: 1425 4594
SbfI CC_TGCA'GG
Cuts at: 3371 3371
Size: 6019
ScaI AGT'ACT
Cuts at: 4267 5979 4267
Size: 1712 4307
SfcI C'TryA_G
Cuts at: 826 1045 2049 3367 4502 5183 5374 826
Size: 219 1004 1318 1135 681 191 1471
Fragments arranged by size:
1471 1318 1135 1004 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6019
SmaI CCC'GGG
Cuts at: 1872 3346 1872
Size: 1474 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4123 5024 5286 820
Size: 197 926 2180 901 262 1553
Fragments arranged by size:
2180 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3810 493
Size: 3317 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6019
SphI G_CATG'C
Cuts at: 1132 3377 1132
Size: 2245 3774
SspI AAT'ATT
Cuts at: 5 52 3943 5
Size: 47 3891 2081
Fragments arranged by size:
3891 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6019
StyI C'CwwG_G
Cuts at: 513 1065 3177 3458 513
Size: 552 2112 281 3074
Fragments arranged by size:
3074 2112 552 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2294 4046 4063 4216 4401 4846 325
Size: 1255 714 1752 17 153 185 445 1498
Fragments arranged by size:
1752 1498 1255 714 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4265 5977 96
Size: 274 80 33 51 1443 2288 1712 138
Fragments arranged by size:
2288 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5979 6011 5979
Size: 32 5987
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6019
XbaI T'CTAG_A
Cuts at: 2686 3004 3355 2686
Size: 318 351 5350
Fragments arranged by size:
5350 351 318
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6019
XmaI C'CCGG_G
Cuts at: 1870 3344 1870
Size: 1474 4545
XmnI GAAnn'nnTTC
Cuts at: 4148 4148
Size: 6019
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3827 276
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI