################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AscI Asp700I AspEI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaI BsiEI BsiWI BsmBI BsmI Bsp1407I BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI Bst1107I BstEII BstPI BstZ17I Bsu36I Cfr10I DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SwaI Tsp45I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 954 GGTACC G^GTACC AccI 241 GTCTAC GT^[AC][GT]AC AccIII 100 TCCGGA T^CCGGA AcyI 211 GGCGTC G[GA]^CG[CT]C Alw44I 873 GTGCAC G^TGCAC ApaI 351 GGGCCC GGGCC^C ApaLI 873 GTGCAC G^TGCAC AseI 589 ATTAAT AT^TAAT AsnI 589 ATTAAT AT^TAAT Asp718I 954 GGTACC G^GTACC AspHI 873 GTGCAC G[AT]GC[AT]^C AspI 169 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 258 GAAGAC GAAGAC(2/6) BbvCI 633 GCTGAGG CCTCAGC(-5/-2) BglI 568 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 423 GCTGAGC GC^T[ACGT]AGC Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC BpuAI 258 GAAGAC GAAGAC(2/6) BsaHI 211 GGCGTC G[GA]^CG[CT]C BseAI 100 TCCGGA T^CCGGA BsiHKAI 873 GTGCAC G[AT]GC[AT]^C BsmAI 613 GTCTC GTCTC(1/5) BsmFI 153 GTCCC GGGAC(10/14) BspDI 676 ATCGAT AT^CGAT BspEI 100 TCCGGA T^CCGGA BssHII 447 GCGCGC G^CGCGC BstBI 778 TTCGAA TT^CGAA BstXI 404 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1181 AGATCC [GA]^GATC[CT] CelII 423 GCTGAGC GC^T[ACGT]AGC ClaI 676 ATCGAT AT^CGAT CpoI 160 CGGTCCG CG^G[AT]CCG DraIII 466 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG Hin1I 211 GGCGTC G[GA]^CG[CT]C HincII 913 GTCAAC GT[CT]^[GA]AC HindII 913 GTCAAC GT[CT]^[GA]AC HphI 672 GGTGA GGTGA(8/7) KpnI 954 GGTACC GGTAC^C MaeIII 485 GTTAC ^GT[ACGT]AC MflI 1181 AGATCC [GA]^GATC[CT] MroI 100 TCCGGA T^CCGGA MseI 590 TTAA T^TAA NcoI 266 CCATGG C^CATGG NspV 778 TTCGAA TT^CGAA PflFI 169 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 589 ATTAAT AT^TAAT PstI 73 CTGCAG CTGCA^G RsrII 160 CGGTCCG CG^G[AT]CCG SapI 788 GAAGAGC GCTCTTC(1/4) SfaNI 69 GATGC GCATC(5/9) SfuI 778 TTCGAA TT^CGAA SphI 800 GCATGC GCATG^C SspI 131 AATATT AAT^ATT Tru9I 590 TTAA T^TAA Tsp509I 592 AATT ^AATT Tth111I 169 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 659 CCAACTATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1181 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AlwNI 37 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 429 CAGCCACTG Aor51HI 341 AGCGCT AGC^GCT 1019 AGCGCT AvaI 509 CCCGGG C^[CT]CG[GA]G 583 CCCGGG BalI 290 TGGCCA TGG^CCA 401 TGGCCA BanII 351 GGGCCC G[GA]GC[CT]^C 791 GAGCCC BfaI 224 CTAG C^TAG 565 CTAG BpmI 206 CTGGAG CTGGAG(16/14) 337 CTGGAG BsaAI 949 TACGTG [CT]AC^GT[GA] 985 TACGTG BsaWI 20 TCCGGT [AT]^CCGG[AT] 100 TCCGGA BseRI 118 GAGGAG GAGGAG(10/8) 388 GAGGAG BsoBI 509 CCCGGG C^[CT]CG[GA]G 583 CCCGGG BsrI 744 CCAGT ACTGG(1/-1) 1194 CCAGT BssSI 469 CTCGTG CACGAG(-5/-1) 647 CTCGTG BstAPI 428 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 875 GCACCCCCTGC BstF5I 18 CATCC GGATG(2/0) 1147 CATCC DdeI 424 CTGAG C^T[ACGT]AG 634 CTGAG EarI 788 GAAGAG CTCTTC(1/4) 925 GAAGAG Eco47III 341 AGCGCT AGC^GCT 1019 AGCGCT FokI 18 CATCC GGATG(9/13) 1147 CATCC HaeII 341 AGCGCT [GA]GCGC^[CT] 1019 AGCGCT HinfI 639 GAATC G^A[ACGT]TC 1046 GAATC Ksp632I 788 GAAGAG CTCTTC(1/4) 925 GAAGAG MaeI 224 CTAG C^TAG 565 CTAG MaeII 950 ACGT A^CGT 986 ACGT MluNI 290 TGGCCA TGG^CCA 401 TGGCCA MscI 290 TGGCCA TGG^CCA 401 TGGCCA MslI 57 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 801 CATGCCCATG NheI 223 GCTAGC G^CTAGC 564 GCTAGC PpuMI 965 AGGACCC [GA]G^G[AT]CC[CT] 1091 AGGTCCT SexAI 170 ACCAGGT A^CC[AT]GGT 836 ACCAGGT SfcI 73 CTGCAG C^T[GA][CT]AG 243 CTACAG SmaI 509 CCCGGG CCC^GGG 583 CCCGGG StuI 714 AGGCCT AGG^CCT 1055 AGGCCT TfiI 639 GAATC G^A[AT]TC 1046 GAATC TspRI 433 CACTG CA[GC]TG[ACGT][ACGT]^ 745 CAGTG XmaCI 509 CCCGGG C^CCGGG 583 CCCGGG XmaI 509 CCCGGG C^CCGGG 583 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanI BbvI BcnI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PvuII RsaI Sau3AI Sau96I ScrFI StyI TaqI TseI TthHB8I