(Circular) (Six-base) MAPSORT of: pFN21AE6332.seq Check: 6706 from: 1 to: 5950 pFN21AE6332 5950 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2247 3293 1937 Size: 310 1046 4594 Fragments arranged by size: 4594 1046 310 Acc65I G'GTAC_C Cuts at: 2959 3271 2959 Size: 312 5638 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 4013 5397 5665 1632 Size: 650 1731 1384 268 1917 Fragments arranged by size: 1917 1731 1384 650 268 AfeI AGC'GCT Cuts at: 2348 3026 2348 Size: 678 5272 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 2047 2439 3693 5265 2047 Size: 392 1254 1572 2732 Fragments arranged by size: 2732 1572 1254 392 ApaI G_GGCC'C Cuts at: 2360 2360 Size: 5950 ApaLI G'TGCA_C Cuts at: 2878 4007 5163 2878 Size: 1129 1156 3665 Fragments arranged by size: 3665 1156 1129 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2595 4504 160 Size: 872 1563 1909 1606 Fragments arranged by size: 1909 1606 1563 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 2514 2588 3275 1870 Size: 73 571 74 687 4545 Fragments arranged by size: 4545 687 571 74 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2609 2695 2759 2773 2893 Size: 325 217 627 822 86 64 14 120 Cuts at: 2893 2959 3007 3271 4726 618 Size: 66 48 264 1455 1842 Fragments arranged by size: 1842 1455 822 627 325 264 217 120 86 66 64 48 14 BanII G_rGCy'C Cuts at: 1391 2360 2800 3269 1391 Size: 969 440 469 4072 Fragments arranged by size: 4072 969 469 440 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 928 Size: 971 371 4608 Fragments arranged by size: 4608 971 371 BbvCI CC'TCA_GC Cuts at: 2639 2639 Size: 5950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4141 4175 1903 Size: 34 50 34 2120 34 3678 Fragments arranged by size: 3678 2120 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2579 4561 136 Size: 107 122 71 631 605 907 1982 1525 Fragments arranged by size: 1982 1525 907 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 2429 3367 2429 Size: 938 5012 Bme1580I G_kGCm'C Cuts at: 1790 2360 2483 2508 2612 2700 2882 4011 5167 Size: 570 123 25 104 88 182 1129 1156 Cuts at: 5167 1790 Size: 2573 Fragments arranged by size: 2573 1156 1129 570 182 123 104 88 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2232 2573 1056 Size: 1176 341 4433 Fragments arranged by size: 4433 1176 341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2363 4610 1239 Size: 993 131 2247 2579 Fragments arranged by size: 2579 2247 993 131 Bpu10I CC'TnA_GC Cuts at: 2639 2639 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4613 882 Size: 617 3114 2219 Fragments arranged by size: 3114 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2956 2992 3741 493 Size: 711 1752 36 749 2702 Fragments arranged by size: 2702 1752 749 711 36 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 3757 4139 4774 Size: 53 83 186 936 684 1540 382 635 Cuts at: 4774 275 Size: 1451 Fragments arranged by size: 1540 1451 936 684 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2025 2105 4383 4781 5055 5202 5613 2025 Size: 80 2278 398 274 147 411 2362 Fragments arranged by size: 2362 2278 411 398 274 147 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2991 3021 1178 Size: 30 165 30 1588 30 4107 Fragments arranged by size: 4107 1588 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2408 1186 Size: 952 270 4728 Fragments arranged by size: 4728 952 270 BseYI C'CCAG_C Cuts at: 2349 2583 2605 2699 2913 5153 5649 2349 Size: 234 22 94 214 2240 496 2650 Fragments arranged by size: 2650 2240 496 234 214 94 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 2309 2852 1163 Size: 573 360 213 543 4261 Fragments arranged by size: 4261 573 543 360 213 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2882 4011 4096 5167 2882 Size: 1129 85 1071 3665 Fragments arranged by size: 3665 1129 1071 85 BsmI GAATG_Cn' Cuts at: 3461 3554 3461 Size: 93 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2360 2483 2508 2612 2700 2800 2882 Size: 399 570 123 25 104 88 100 82 Cuts at: 2882 3269 4011 4096 5167 1391 Size: 387 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 399 387 123 104 100 88 85 82 25 BspEI T'CCGG_A Cuts at: 2105 5613 2105 Size: 3508 2442 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2643 2931 3305 844 Size: 1484 315 288 374 3489 Fragments arranged by size: 3489 1484 374 315 288 BsrBI CCG'CTC Cuts at: 1394 1823 3837 1394 Size: 429 2014 3507 Fragments arranged by size: 3507 2014 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4594 4781 1700 Size: 110 2784 187 2869 Fragments arranged by size: 2869 2784 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 2452 1925 Size: 527 5423 BssSI C'ACGA_G Cuts at: 2474 2652 4010 5022 2474 Size: 178 1358 1012 3402 Fragments arranged by size: 3402 1358 1012 178 BstAPI GCAn_nnn'nTGC Cuts at: 794 2439 2886 794 Size: 1645 447 3858 Fragments arranged by size: 3858 1645 447 BstBI TT'CG_AA Cuts at: 2784 4754 2784 Size: 1970 3980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2416 Size: 5950 BstYI r'GATC_y Cuts at: 1972 3186 3280 3647 4032 4049 5501 5587 5599 Size: 1214 94 367 385 17 1452 86 12 Cuts at: 5599 5945 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1214 385 367 346 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2162 2169 2271 2409 513 Size: 552 1097 7 102 138 4054 Fragments arranged by size: 4054 1097 552 138 102 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2087 487 Size: 683 56 861 4350 Fragments arranged by size: 4350 861 683 56 BtsI GCAGTG_nn' Cuts at: 2437 3466 4260 4280 2437 Size: 1029 794 20 4107 Fragments arranged by size: 4107 1029 794 20 ClaI AT'CG_AT Cuts at: 1554 2682 1554 Size: 1128 4822 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DraIII CAC_nnn'GTG Cuts at: 2476 2476 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2295 2382 Size: 54 1425 50 275 18 222 243 87 Cuts at: 2382 2406 3397 4286 5915 8 Size: 24 991 889 1629 43 Fragments arranged by size: 1629 1425 991 889 275 243 222 87 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2788 2925 3880 1353 Size: 1435 137 955 3423 Fragments arranged by size: 3423 1435 955 137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2282 2363 4013 4610 5397 5665 Size: 393 600 50 81 1650 597 787 268 Cuts at: 5665 1239 Size: 1524 Fragments arranged by size: 1650 1524 787 600 597 393 268 81 50 EcoO109I rG'GnC_Cy Cuts at: 2254 2356 2971 3097 2254 Size: 102 615 126 5107 Fragments arranged by size: 5107 615 126 102 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2350 3028 5097 1678 Size: 672 678 2069 2531 Fragments arranged by size: 2531 2069 678 672 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3178 3210 4591 4623 4665 Size: 32 135 32 1606 32 1381 32 42 Cuts at: 4665 4697 1373 Size: 32 2626 Fragments arranged by size: 2626 1606 1381 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2920 3294 3540 669 Size: 988 281 982 374 246 3079 Fragments arranged by size: 3079 988 982 374 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 2963 3275 2963 Size: 312 5638 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5064 5248 1305 Size: 3759 184 2007 Fragments arranged by size: 3759 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 2408 10 Size: 54 1425 343 465 111 3552 Fragments arranged by size: 3552 1425 465 343 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2066 2810 3908 4267 4426 518 Size: 648 843 57 744 1098 359 159 2042 Fragments arranged by size: 2042 1098 843 744 648 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2044 2083 2362 2404 2913 3366 4043 5191 Size: 438 39 279 42 509 453 677 1148 Cuts at: 5191 5436 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 509 453 438 279 245 42 39 NcoI C'CATG_G Cuts at: 513 1065 2271 513 Size: 552 1206 4192 Fragments arranged by size: 4192 1206 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NheI G'CTAG_C Cuts at: 1052 2228 2569 1052 Size: 1176 341 4433 Fragments arranged by size: 4433 1176 341 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2452 2809 3077 3308 4853 1132 Size: 97 1223 357 268 231 1545 2229 Fragments arranged by size: 2229 1545 1223 357 268 231 97 PasI CC'CwG_GG Cuts at: 1348 2976 1348 Size: 1628 4322 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PfoI T'CCnGG_A Cuts at: 2220 2220 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2971 3097 2971 Size: 126 5824 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 2356 2356 Size: 5950 PstI C_TGCA'G Cuts at: 830 2082 3302 830 Size: 1252 1220 3478 Fragments arranged by size: 3478 1252 1220 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2044 2083 2404 2044 Size: 39 321 5590 Fragments arranged by size: 5590 321 39 RsrII CG'GwC_CG Cuts at: 2166 2166 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SapI GCTCTTCn'nnn_ Cuts at: 2788 2788 Size: 5950 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SexAI A'CCwGG_T Cuts at: 2175 2841 2175 Size: 666 5284 SfcI C'TryA_G Cuts at: 826 1045 2078 2248 3298 4433 5114 5305 826 Size: 219 1033 170 1050 1135 681 191 1471 Fragments arranged by size: 1471 1135 1050 1033 681 219 191 170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 2516 2590 3277 1872 Size: 644 74 687 4545 Fragments arranged by size: 4545 687 644 74 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 2809 3308 1132 Size: 1677 499 3774 Fragments arranged by size: 3774 1677 499 SspI AAT'ATT Cuts at: 5 52 2138 3874 5 Size: 47 2086 1736 2081 Fragments arranged by size: 2086 2081 1736 47 StuI AGG'CCT Cuts at: 1517 2721 3062 1517 Size: 1204 341 4405 Fragments arranged by size: 4405 1204 341 StyI C'CwwG_G Cuts at: 513 1065 2271 2388 2963 3389 513 Size: 552 1206 117 575 426 3074 Fragments arranged by size: 3074 1206 575 552 426 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2911 3977 3994 4147 4332 4777 325 Size: 1255 1331 1066 17 153 185 445 1498 Fragments arranged by size: 1498 1331 1255 1066 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4196 5908 96 Size: 274 80 33 51 1443 2219 1712 138 Fragments arranged by size: 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2991 3023 5910 5942 2991 Size: 32 2887 32 2999 Fragments arranged by size: 2999 2887 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2177 1316 Size: 861 5089 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2671 2671 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 2514 2588 3275 1870 Size: 644 74 687 4545 Fragments arranged by size: 4545 687 644 74 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SfiI SfoI SrfI SwaI