(Linear) (Six-base) MAPSORT of: KIEE6332.seq Check: 1740 from: 1 to: 1245 KIEE6332.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 242 1245 Size: 242 1003 Acc65I G'GTAC_C Cuts at: 0 954 1245 Size: 954 291 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 1245 Size: 277 968 AfeI AGC'GCT Cuts at: 0 343 1021 1245 Size: 343 678 224 Fragments arranged by size: 678 343 224 AlwNI CAG_nnn'CTG Cuts at: 0 42 434 1245 Size: 42 392 811 Fragments arranged by size: 811 392 42 ApaI G_GGCC'C Cuts at: 0 355 1245 Size: 355 890 ApaLI G'TGCA_C Cuts at: 0 873 1245 Size: 873 372 AseI AT'TA_AT Cuts at: 0 590 1245 Size: 590 655 AvaI C'yCGr_G Cuts at: 0 509 583 1245 Size: 509 74 662 Fragments arranged by size: 662 509 74 BanI G'GyrC_C Cuts at: 0 604 690 754 768 888 954 1002 1245 Size: 604 86 64 14 120 66 48 243 Fragments arranged by size: 604 243 120 86 66 64 48 14 BanII G_rGCy'C Cuts at: 0 355 795 1245 Size: 355 440 450 Fragments arranged by size: 450 440 355 BbsI GAAGACnn'nnnn_ Cuts at: 0 265 1245 Size: 265 980 BbvCI CC'TCA_GC Cuts at: 0 634 1245 Size: 634 611 BglI GCCn_nnn'nGGC Cuts at: 0 574 1245 Size: 574 671 BlpI GC'TnA_GC Cuts at: 0 424 1245 Size: 424 821 Bme1580I G_kGCm'C Cuts at: 0 355 478 503 607 695 877 1245 Size: 355 123 25 104 88 182 368 Fragments arranged by size: 368 355 182 123 104 88 25 BmtI G_CTAG'C Cuts at: 0 227 568 1245 Size: 227 341 677 Fragments arranged by size: 677 341 227 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 358 1245 Size: 227 131 887 Fragments arranged by size: 887 227 131 Bpu10I CC'TnA_GC Cuts at: 0 634 1245 Size: 634 611 BsaAI yAC'GTr Cuts at: 0 951 987 1245 Size: 951 36 258 Fragments arranged by size: 951 258 36 BsaHI Gr'CG_yC Cuts at: 0 212 1245 Size: 212 1033 BsaWI w'CCGG_w Cuts at: 0 20 100 1245 Size: 20 80 1145 Fragments arranged by size: 1145 80 20 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 986 1016 1245 Size: 986 30 229 Fragments arranged by size: 986 229 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 133 403 1245 Size: 133 270 842 Fragments arranged by size: 842 270 133 BseYI C'CCAG_C Cuts at: 0 344 578 600 694 908 1245 Size: 344 234 22 94 214 337 Fragments arranged by size: 344 337 234 214 94 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 304 847 1245 Size: 91 213 543 398 Fragments arranged by size: 543 398 213 91 BsiHKAI G_wGCw'C Cuts at: 0 877 1245 Size: 877 368 Bsp1286I G_dGCh'C Cuts at: 0 355 478 503 607 695 795 877 1245 Size: 355 123 25 104 88 100 82 368 Fragments arranged by size: 368 355 123 104 100 88 82 25 BspEI T'CCGG_A Cuts at: 0 100 1245 Size: 100 1145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 323 638 926 1245 Size: 323 315 288 319 Fragments arranged by size: 323 319 315 288 BssHII G'CGCG_C Cuts at: 0 447 1245 Size: 447 798 BssSI C'ACGA_G Cuts at: 0 469 647 1245 Size: 469 178 598 Fragments arranged by size: 598 469 178 BstAPI GCAn_nnn'nTGC Cuts at: 0 434 881 1245 Size: 434 447 364 Fragments arranged by size: 447 434 364 BstBI TT'CG_AA Cuts at: 0 779 1245 Size: 779 466 BstXI CCAn_nnnn'nTGG Cuts at: 0 411 1245 Size: 411 834 BstYI r'GATC_y Cuts at: 0 1181 1245 Size: 1181 64 BtgI C'CryG_G Cuts at: 0 157 164 266 404 1245 Size: 157 7 102 138 841 Fragments arranged by size: 841 157 138 102 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 82 1245 Size: 82 1163 BtsI GCAGTG_nn' Cuts at: 0 432 1245 Size: 432 813 ClaI AT'CG_AT Cuts at: 0 677 1245 Size: 677 568 DraIII CAC_nnn'GTG Cuts at: 0 471 1245 Size: 471 774 EaeI y'GGCC_r Cuts at: 0 47 290 377 401 1245 Size: 47 243 87 24 844 Fragments arranged by size: 844 243 87 47 24 EarI CTCTTCn'nnn_ Cuts at: 0 783 920 1245 Size: 783 137 325 Fragments arranged by size: 783 325 137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 277 358 1245 Size: 227 50 81 887 Fragments arranged by size: 887 227 81 50 EcoO109I rG'GnC_Cy Cuts at: 0 249 351 966 1092 1245 Size: 249 102 615 126 153 Fragments arranged by size: 615 249 153 126 102 HaeII r_GCGC'y Cuts at: 0 345 1023 1245 Size: 345 678 222 Fragments arranged by size: 678 345 222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1173 1205 1245 Size: 1173 32 40 Fragments arranged by size: 1173 40 32 HincII GTy'rAC Cuts at: 0 915 1245 Size: 915 330 KpnI G_GTAC'C Cuts at: 0 958 1245 Size: 958 287 MscI TGG'CCA Cuts at: 0 292 403 1245 Size: 292 111 842 Fragments arranged by size: 842 292 111 MslI CAynn'nnrTG Cuts at: 0 61 805 1245 Size: 61 744 440 Fragments arranged by size: 744 440 61 MspA1I CmG'CkG Cuts at: 0 39 78 357 399 908 1245 Size: 39 39 279 42 509 337 Fragments arranged by size: 509 337 279 42 39 39 NcoI C'CATG_G Cuts at: 0 266 1245 Size: 266 979 NheI G'CTAG_C Cuts at: 0 223 564 1245 Size: 223 341 681 Fragments arranged by size: 681 341 223 NspI r_CATG'y Cuts at: 0 447 804 1072 1245 Size: 447 357 268 173 Fragments arranged by size: 447 357 268 173 PasI CC'CwG_GG Cuts at: 0 971 1245 Size: 971 274 PfoI T'CCnGG_A Cuts at: 0 215 1245 Size: 215 1030 PpuMI rG'GwC_Cy Cuts at: 0 966 1092 1245 Size: 966 126 153 Fragments arranged by size: 966 153 126 PspOMI G'GGCC_C Cuts at: 0 351 1245 Size: 351 894 PstI C_TGCA'G Cuts at: 0 77 1245 Size: 77 1168 PvuII CAG'CTG Cuts at: 0 39 78 399 1245 Size: 39 39 321 846 Fragments arranged by size: 846 321 39 39 RsrII CG'GwC_CG Cuts at: 0 161 1245 Size: 161 1084 SapI GCTCTTCn'nnn_ Cuts at: 0 783 1245 Size: 783 462 SexAI A'CCwGG_T Cuts at: 0 170 836 1245 Size: 170 666 409 Fragments arranged by size: 666 409 170 SfcI C'TryA_G Cuts at: 0 73 243 1245 Size: 73 170 1002 Fragments arranged by size: 1002 170 73 SmaI CCC'GGG Cuts at: 0 511 585 1245 Size: 511 74 660 Fragments arranged by size: 660 511 74 SphI G_CATG'C Cuts at: 0 804 1245 Size: 804 441 SspI AAT'ATT Cuts at: 0 133 1245 Size: 133 1112 StuI AGG'CCT Cuts at: 0 716 1057 1245 Size: 716 341 188 Fragments arranged by size: 716 341 188 StyI C'CwwG_G Cuts at: 0 266 383 958 1245 Size: 266 117 575 287 Fragments arranged by size: 575 287 266 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 906 1245 Size: 906 339 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 986 1018 1245 Size: 986 32 227 Fragments arranged by size: 986 227 32 Tth111I GACn'n_nGTC Cuts at: 0 172 1245 Size: 172 1073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 666 1245 Size: 666 579 XmaI C'CCGG_G Cuts at: 0 509 583 1245 Size: 509 74 662 Fragments arranged by size: 662 509 74 Enzymes that do cut: AccI Acc65I AcuI AfeI AlwNI ApaI ApaLI AseI AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmtI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII KpnI MscI MslI MspA1I NcoI NheI NspI PasI PfoI PpuMI PspOMI PstI PvuII RsrII SapI SexAI SfcI SmaI SphI SspI StuI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BplI BpuEI BsaI BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BstEII BstZ17I Bsu36I CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SwaI TatI XbaI XhoI XmnI ZraI